检索结果-南通市图书馆

Effect of β-blocker therapy in diabetic patients with stable coronary heart disease: a meta-analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2019年第3期16卷 291-297页

作者： Aaqib H Malik Suchith Shetty kausik Kar Ramzi El Accaoui Department of Medicine Westchester Medical Center and New York Medical CollegeValhallaNYUSA Division of Cardiology Department of MedicineUniversity of Iowa Health CareCarver College of MedicineIowaUSA

Background β-blocker (BB) therapy is a cornerstone for the treatment of coronary heart disease (CHD).The evidence of the benefit from long-term BB therapy in diabetic patients with stable CHD is scarce.This meta-analysis summarizes the evidence relating to the BB therapy in diabetic patients with stable CHD.Methods A meta-analysis was performed according to PRISMA and MOOSE guidelines for reporting of systematic reviews of observational studies.PubMed,Embase,and Cochrane central were searched and two authors independently screened studies for eligibility.The quality of studies was assessed with the Newcastle Ottawa scale.The primary outcome of interest was all-cause mortality,cardiovascular (CV) mortality and major adverse cardiovascular events (MACE) in diabetic patients with and without BB therapy.A generic inverse variance model was used to pool odds ratio or hazards ratio from included studies to calculate the overall effect estimate.The significance threshold was set at P-value < 0.05.Heterogeneity was assessed by I2.Results Four non-randomized studies with 9515 participants were selected for the analyses.Four studies were post-hoc analyses of randomized controlled trials,and one article was an analysis of a nationally representative survey.In a fixed effects model,BB therapy in diabetic patients with stable CHD was found to be associated with increased risk of CV mortality,and MACE (27% and 32% respectively;P-value < 0.05) and was not associated with a reduction in all-cause mortality (HR 1.12;95% CI: 0.94–1.33;P-value = 0.22).Conclusion BB therapy in diabetic patients with stable CHD appears to be linked to higher mortality.Large randomized trials are needed in this population to confirm these findings.

关键词： β-blockers Cardiovascular mortality Diabetes MACE

Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: drug-DNA interaction and signaling cascade

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2014年第2期12卷 102-114页

作者： Samrat Ghosh kausik Bishayee Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani

OBJECTIVE： Oleanolic acid （OA） has been reported to have anticancer effects, but the extent of its cytotoxicity, its ability to interact with nuclear DNA, its action against skin melanoma, as well as the molecular mechanism of its action against cell proliferation and in support of cell death are still unexplored. This led us to examine the efficacy of OA, a bioactive compound isolated from Phytolacca decandra, on these issues in the present investigation. METHODS： Studies related to analyses of cell viability, drug-DNA interaction, cell proliferation, cell cycle and epidermal growth factor receptor （EGFR） activity were performed. To investigate whether cells undergo apoptosis, studies like fluorescence microscopy, poly （ADP-ribose） polymerase （PARP） degradation, annexin V-fluorescein isothiocyanate/propidium iodide assay, alteration in mitochondrial membrane potential and activity of some relevant signaling proteins were performed. RESULTS： OA displayed a minimal and negligible cytotoxic effect on normal HaCaT cells （skin keratinocytes） and peripheral blood mononuclear cells but by contrast it reduced A375 cell viability significantly. OA interacted with nuclear DNA quickly after exposure. It acted as an anti- proliferative agent. It suppressed EGFR activity. OA administration led the cells to mitochondria- dependent caspase 3-mediated apoptosis. CONCLUSION： OA interacts with cellular DNA, inhibits proliferation possibly through modulating EGFR activity and induces mitochondria-dependent caspase 3-mediated apoptosis in A375 cells which would qualify it as a potent anticancer agent.

关键词： oleanolic acid skin melanoma drug-DNA interaction proliferation epidermal growth factor receptor apoptosis

Low doses of ethanolic extract of Boldo(Peumus boldus) can ameliorate toxicity generated by cisplatin in normal liver cells of mice in vivo and in WRL-68 cells in vitro, but not in cancer cells in vivo or in vitro

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2014年第5期12卷 425-438页

作者： Jesmin Mondal kausik Bishayee Ashis Kumar Panigrahi Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of Zoology University of Kalyani Fisheries and Aquaculture Laboratory Department of Zoology University of Kalyani

OBJECTIVE： Use of cisplatin, a conventional anticancer drug, is restricted because it generates strong hepatotoxicity by accumulating in liver. Therefore its anticancer potential can only be fully exploited if its own toxicity is considerably reduced. Towards this goal, ethanolic extract of the plant, Boldo （Peumus boldus）, known for its antihepatotoxic effects, was used simultaneously with cisplatin, to test its ability to reduce cisplatin＇s cytotoxicity without affecting its anticancer potential. METHODS： The cytotoxicity of Boldo extract （BE） and cisplatin, administered alone and in combination, was determined in three cancer cell lines （A549, HeLa, and HepG2） and in normal liver cells （WRL-68）. Drug-DNA interaction, DNA damage, cell cycle, apoptosis, reactive oxygen species （ROS） and mitochondrial membrane potential （MMP, Aψ） were also studied. Hepatotoxicity and antioxidant activity levels were determined by alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and glutathione assays in mice. The cytotoxicity of related proteins was tested by Western blotting. RESULTS： Co-administration of BE and cisplatin increased viability of normal cells, but had no effect on the viability of cancer cells. Boldo protected liver from damage and normalized different antioxidant enzyme levels in vivo and also reduced ROS and re-polarized MMP in vitro. Bax and cytochrome c translocation was reduced with caspase 3 down-regulation. Further, a drug- DNA interaction study revealed that BE reduced cisplatin＇s DNA-binding capacity, resulting in a reduction in DNA damage. CONCLUSION： Results indicated that a low dose of BE could be used beneficially in combination with cisplatin to reduce its toxicity without hampering cisplatin＇s anticancer effect. These findings siqnify a potential future use of BE in cancer therapy.

关键词： drug-related toxicity Peumus boldus plant ethanolic extract cytotoxicity liver anti-oxidant

Non-invasive determination of murine placental and foetal functional parameters with multispectral optoacoustic tomography

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2019年第1期8卷 553-562页

作者： kausik Basak Xose Luis Dean-Ben Sven Gottschalk Michael Reiss Daniel Razansky Faculty of Medicine Technical University MunichMunichGermany Institute for Biological and Medical Imaging Helmholtz Center MunichNeuherbergGermany Faculty of Medicine and Institute of Pharmacology and Toxicology University of ZurichZurichSwitzerland Institute for Biomedical Engineering and Department of Information Technology and Electrical Engineering ETH ZurichZurichSwitzerland Institute of Advanced Studies and Research JIS UniversityKolkataWest BengalIndia

Despite the importance of placental function in embryonic development,it remains poorly understood and challenging to characterize,primarily due to the lack of non-invasive imaging tools capable of monitoring placental and foetal oxygenation and perfusion parameters during pregnancy.We developed an optoacoustic tomography approach for real-time imaging through entire ~4 cm cross-sections of pregnant mice.Functional changes in both maternal and embryo regions were studied at different gestation days when subjected to an oxygen breathing challenge and perfusion with indocyanine green.Structural phenotyping of the cross-sectional scans highlighted different internal organs,whereas multi-wavelength acquisitions enabled non-invasive label-free spectroscopic assessment of blood-oxygenation parameters in foeto-placental regions,rendering a strong correlation with the amount of oxygen administered.Likewise,the placental function in protecting the embryo from extrinsically administered agents was substantiated.The proposed methodology may potentially further serve as a probing mechanism to appraise embryo development during pregnancy in the clinical setting.

关键词： rendering placental sectional

Successful Antenatal Diagnosis and Management of a Rare Case of Congenital Fetal Choledochal Cyst

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Maternal-Fetal Medicine 2021年第3期3卷 225-228页

作者： Indu Lata Ankur Mandelia kausik Mandal Department of Maternal and Reproductive Health Sanjay Gandhi Post Graduate Institute of Medical SciencesLucknowUttar Pradesh 226014India Department of Pediatric Surgery Sanjay Gandhi Post Graduate Institute of Medical SciencesLucknowUttar Pradesh 226014India Department of Medical Genetics Sanjay Gandhi Post Graduate Institute of Medical SciencesLucknowUttar Pradesh 226014India

Choledochal cysts is a rare congenital cystic dilatation of common biliary duct.The most common prenatal diagnosed form type-1(85%-90%),consists of fusiform dilation of the common bile duct.There is communication between the bile duct and the cyst.In antenatal period diagnosis may be made by the presence of a cyst in the upper right side of the fetal abdomen on ultrasound.To differentiate it with other cystic lesions prenatal ultrasonography(USG),three-dimensional USG and magnetic resonance imaging may be helpful.By early diagnosis and prompt removal of cyst in postnatal period long term complications like development of biliary cirrhosis,portal hypertension,calculi formation or adenocarcinoma can be avoided in later life.The operative mortality is about 10%.We are reporting a case of antenatal diagnosis type I congenital choledochal cysts at 21 weeks,its follow up and successful postnatal management.

关键词： Choledochal cyst Congenital Fetal Management Prenatal diagnosis

Study of a collisionless magnetized plasma sheath with nonextensively distributed species

在线全文

学校读者我要写书评

暂无评论

Plasma Science and Technology 2023年第12期25卷 14-23页

作者： R PAUL K DEKA G SHARMA R MOULICK S ADHIKARI S S kausik B K SAIKIA Centre of Plasma Physics Institute for Plasma ResearchAssam 782402India Institute for Energy Technology Instituttveien 8Kjeller 2007Norway

A weakly magnetized sheath for a collisionless, electronegative plasma comprising positive ions,electrons, and negative ions is investigated numerically using the fluid approach. The electrons are considered to be non-Maxwellian in nature and are described by Tsalli's distribution. Such electrons have a substantial effect on the sheath properties. The study also reveals that non-Maxwellian distribution is the most realistic description for negative ions in the presence of an oblique magnetic field. In addition to the negative ion temperature, the sheath potential is also affected by the nonextensive parameters. The present research finds application in the plasma processing and semiconductor industry as well as in space plasmas.

关键词： magnetised plasma sheath electronegative plasma non-extensive distribution

Large-signal characterization of DDR silicon IMPATTs operating in millimeter-wave and terahertz regime

在线全文

学校读者我要写书评

暂无评论

Journal of Semiconductors 2013年第10期34卷 46-53页

作者： Aritra Acharyya Jit Chakraborty kausik Das Subir Datta Pritam De Suranjana Banerjee J.P.Banerjee Institute of Radio Physics and Electronics University of Calcutta92APC RoadKolkata 700009India Supreme Knowledge Foundation Group of Institutions Sir J.C.Bose School of Engineering1Khan RoadMankunduHooghlyWest Bengal 712139India Academy of Technology West Bengal University of TechnologyAdisaptagramHooghly 712121West BengalIndia

The authors have carried out the large-signal characterization ofsilicon-based double-drift region （DDR） impact avalanche transit time （IMPATT） devices designed to operate up to 0.5 THz using a large-signal simulation method developed by the authors based on non-sinusoidal voltage excitation. The effect of band-to-band tunneling as well as parasitic series resistance on the large-signal properties of DDR Si IMPATTs have also been studied at different mm-wave and THz frequencies. Large-signal simulation results show that DDR Si IMPATT is capable of delivering peak RF power of 633.69 mW with 7.95% conversion efficiency at 94 GHz for 50% voltage modulation, whereas peak RF power output and efficiency fall to 81.08 mW and 2.01% respectively at 0.5 THz for same voltage modulation. The simulation results are compared with the experimental results and are found to be in close agreement.

关键词： band to band tunneling DDR silicon IMPATTs large-signal simulation millimeter-wave series resistance terahertz regime

Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines:signalling pathway and drug-DNA interaction

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2013年第6期11卷 405-415页

作者： Avijit Paul Sreemanti Das Jayeeta Das Asmita Samadder kausik Bishayee Ratan Sadhukhan Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Department of Biochemistry and Biophysics University of Kalyani

OBJECTIVE： To test if myricanone （02H2405）, a cyclic diarylheptanoid, has anticancer effects on two different cancer cell lines HeLa and PC3. The present study was conducted with a note on the drug-DNA interaction and apoptotic signalling pathway. METHODS： Several studies like cytotoxicity, nuclear damage, annexin-V-fluorescein isothiocyanate （FITC）/propidium iodide （PI）-Iabelled apoptotic assay and cell cycle arrest, immunoblot and reverse transcriptase-polymerase chain reaction （RT-PCR） were used following standard protocols. Circular dichroism （CD） spectroscopy was also done to evaluate whether myricanone effectively interacted with DNA to bring about conformational changes that could strongly inhibit the cancer cell proliferation. RESULTS： Myricanone showed a greater cytotoxic effect on PC3 cells than on HeLa cells. Myricanone promoted G0/G arrest in HeLa cells and S phase arrest in PC3 cells. Nuclear condensation and annexin V-FITC/PI studies revealed that myricanone promoted apoptotic cell death. CD spectroscopic data indicated that myricanone had an interaction with calf thymus DNA that changed DNA structural conformation. RT-PCR and immunoblot studies revealed that myricanone activated the apoptotic signalling cascades through down-regulation of transcription factors like nuclear factor-KB （NF-KB）（p65）, and signal transducers and activators of transcription 3 （STAT3）; cell cycle regulators like cyclin D1, and survivin and other signal proteins like Bcl-2 and up-regulation of Bax, caspase-9 and caspase-3. CONCLUSION： Myricanone induced apoptosis in both types of cancer cells by triggering caspase activation, and suppression of cell proliferation by down-regulation of NF-KB and STAT3 signalling cascades, which makes it a suitable candidate for possible use in the formulation of therapeutic alent for combatin cancer.

关键词： KEYWORDS： Myrica diarylheptanoids plant extracts apoptosis NF-KB STAT3 transcriptionfactor signal transduction

Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2013年第2期11卷 116-124页

作者： Samrat Ghosh kausik Bishayee Avijit Paul Avinaba Mukherjee Sourav Sikdar Debrup Chakraborty Naoual Boujedaini Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of Zoology University of Kalyani Boiron Laboratories

OBJECTIVE： Preventive measures against skin melanoma like chemotherapy are useful but suffer from chronic side effects and drug resistance. Ethanolic extract of Phytolacca decandra （PD）, used in homeopathy for the treatment of various ailments like chronic rheumatism, regular conjunctivitis, psoriasis, and in some skin diseases was tested for its possible anticancer potential. METHODS： Cytotoxicity of the drug was tested by conducting 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide assay on both normal （peripheral blood mononuclear cells） and A375 cells. Fluorescence microscopic study of 4＇,6-diamidino-2-phenylindole dihydrochloride-stained cells was conducted for DNA fragmentation assay, and changes in cellular morphology, if any, were also recorded. Lactate dehydrogenase activity assay was done to evaluate the percentages of apoptosis and necrosis. Reactive oxygen species （ROS） accumulation, if any, and expression study of apoptotic genes also were evaluated to pin-point the actual events of apoptosis. RESULTS： Results showed that PD administration caused a remarkable reduction in proliferation of A375 cells, without showing much cytotoxicity on peripheral blood mononuclear cells. Generation of ROS and DNA damage, which made the cancer cells prone to apoptosis, were found to be enhanced in PD-treated cells. These results were duly supported by the analytical data on expression of different cellular and nuclear proteins, as for example, by down- regulation of Akt and Bcl-2, up-regulation of p53, Bax and caspase 3, and an increase in number of cell deaths by apoptosis in A375 cells. CONCLUSION： Overall results demonstrate anticancer potentials of PD on A375 cells through activation of caspase-mediated signaling and ROS generation.

关键词： Phytolacca decandra skin neoplasms reactive oxygen species apoptosis gene expression