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Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner

World journal of Gastroenterology 2012年第26期18卷 3389-3399页

作者： Renee j Phillips karla j helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard School of Molecular and Biomedical Science The University of Adelaide5000 AdelaideAustralia Centre for Cancer Biology SA Pathology 5000 AdelaideAustralia Drug Health Services Royal Prince Alfred Hospital2050 CamperdownAustralia

AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain *** variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell *** expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in ***-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour *** of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and ***:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B *** variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver *** of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture *** of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine *** the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative *** expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative ***:This study demonstrates that OPN plays a significant role in the proliferation of HCC thro

关键词： Osteopontin Hepatocellular carcinoma CD44 antigen Nude mice Xenograft

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Epidemiology and Clinical Features of Mycobacterium avium Complex Pulmonary Disease in a Brazilian Amazon Cohort

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Advances in Microbiology 2024年第6期14卷 287-302页

作者： jose Tadeu Colares Monteiro Adriana Rodrigues Barretto Ruan Seguin Azevedo Quaresma Bianca Miranda Gouveia Ismari Perini Furlaneto Victória Menezes da Costa Yan Corrêa Rodrigues Layana Rufino Ribeiro Ricardo j. de Paula Souza e Guimarães Alex Brito Souza Ana Roberta Fusco da Costa karla Valéria Batista Lima joao de Barros Barreto University Hospital Federal University of Par Belm Brazil Center for Biological and Health Sciences Ph.D. Program in Parasitic Biology in the Amazon Region (PPGBPA) State University of Par (UEPA) Belm Brazil Department of Medicine Centro Universitrio do Par CESUPA Belm Brazil Bacteriology and Mycology Section Evandro Chagas Institute (SABMI/IEC) Ministry of Health Ananindeua Brazil Geoprocessing Laboratory Evandro Chagas Institute/SVSA/MS Ananineua Brazil

The incidence of Mycobacterium avium complex pulmonary disease (MACPD) has been globally increasing. The present study aims to provide a comprehensive analysis of clinical characteristics and treatment response among patients in the Amazon region of Brazil and conduct a retrospective cohort study at a prominent referral hospital in the State of Par, Brazil, from 2012 to 2020. The M. avium group represented 58.9% of cases, followed by M. intracellulare (35.7%), M. colombiense (3.6%), and M. chimaera (1.8%). The majority of patients (73.2%) were female and with an average age of 63 years. Primary clinical manifestations included persistent cough and sputum production. The Charlson comorbidity index (CCI) averaged 2.47, with 36.3% of patients having a CCI score equal to higher than 3. Notably, over 70% of patients had received treatment for pulmonary tuberculosis before initiating treatment for MAC infection. The fibrocavitary radiologic form was the most prevalent (55.4%), frequently exhibiting a bilateral distribution (53.6%). Antimicrobial susceptibility revealed a significant prevalence of MAC resistance to drugs in the therapeutic regimen. Despite this, most of the patients experienced clinical improvement (50%). In conclusion, this study highlights a higher prevalence of MAC infections among middle-aged women, with a history of pulmonary tuberculosis treatment and presenting the fibrocavitary radiological form was predominant.

关键词： MACDP Nontuberculous Mycobacteria Mycobacterium avium Lung Disease

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Predicting adolescent perfectionism: The role of socio-demographic traits, personal relationships, and media

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World journal of Clinical Cases 2022年第1期10卷 189-204页

作者： Goran Livazović karla Kuzmanović Department of Pedagogy University of J.J.StrossmayerOsijek 31000Osječko-baranjskaCroatia Study Programme in Pedagogy Faculty of Humanities and Social SciencesOsijek 31000Osječko-baranjskaCroatia

BACKGROUND The present study investigated perfectionism from the bioecological model perspective as a multidimensional construct manifested in forms of excessively high personal standards,exaggerated worries about personal mistakes,doubt in one’s performance,oversized order and organization emphasis,and the importance of parental valuations and *** To investigate the relation between perfectionism;the quality of family,peer,and college relationships;and media usage and content *** The research was implemented in 2020 with 203 students(134 female,66%)aged 18-25 years,enrolled at the University of Osijek in *** questionnaire had five parts:A)sociodemographic data;B)the Multidimensional Perfectionism Scale;C)the“general life satisfaction”and“current situational life satisfaction”scale;D)family,peer,and college relationships quality scale;and E)media usage and content interests *** of the implemented instruments showed satisfactory reliability.A hierarchical regression analysis was implemented with the aim of establishing significant perfectionism *** Age and gender were significant predictors of *** with lower family relationship quality reported higher parental expectations and complaining as well as significantly higher doubts in personal performance and concern about ***,a lower peer relationship quality predicted doubts in personal performance and stronger concerns about *** quality of college relationships positively predicted higher perfectionist personal standards and *** life satisfaction predicted higher concerns about mistakes,while current situational life satisfaction predicted higher levels of perfectionist *** usage intensity had no significant *** interest in information-educational media predicted higher personal standards as well as concern about mistakes and *** interests in entertainment

关键词： Perfectionism Family Peers Colleagues Life satisfaction Media content

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Interiors an introduction karla j. Nielson, David A. Taylor. 3rd ed.

Interiors an introduction Karla J. Nielson, David A. Taylor.

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2002年

作者： karla j. Nielson David A. Taylor.

来源：畅想之星图书光盘版（南... 评论

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Applying multi-criteria analysis to select the most appropriate broiler rearing environment

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Information Processing in Agriculture 2021年第2期8卷 205-218页

作者： karla A.O.Lima Irenilza A.Nääs Daniella j.Moura Rodrigo G.Garcia Angélica S.Mendes College of Agricultural Engineering The State University of CampinasAve.Cândido Rondon501Barão Geraldo13083-875 CampinasSPBrazil College of Agrarian Sciences The Federal University of Grande DouradosRod Dourados-Itahumkm 1279804-970 DouradosMSBrazil College of Animal Sciences The Technological Federal University of ParanáRod Boa Esperançakm 0485660-000 Dois VizinhosPRBrazil

The present study aimed to assess the most appropriate rearing system for broiler housing in tropical regions using the Analytical Hierarchy Process(AHP).The research was done using data sampling from a field study carried out in eight mechanically-ventilated commercial broiler *** had positive ventilation(open-sided conventional system),and the other four had negative pressure systems,which were named according to the curtains color and light management(blue-house with blue curtains,and dark-house with black side curtains).The data from the field research(rearing dry-bulb temperature and relative humidity;ammonia and carbon dioxide concentrations;energy use;litter type and quality;and broiler performance)were organized,and the multi-criteria analysis AHP was *** the process,the decision-maker must define the problem and chose the criteria to evaluate the alternatives to make a suitable *** alternative selected needs to be according to the stakeholders’*** alternatives consisted of houses using three ventilation systems(conventional,blue-house,and dark-house).The different chosen criteria were the thermal and aerial environment,broiler productivity,energy consumption,and litter *** selected data collected from the field experiment and from current literature to subsidize the selection of the *** computation was done using field data results,knowledge from existing literature,and the expertise of three *** the first time,the AHP technique is applied to select the most suitable broiler housing,considering the complex variables involved in the *** indicated the conventional system as the most appropriate *** of the conventional system showed the highest scores on most criteria in the pairwise *** considered broiler performance,gasses concentration,and electricity cost,providing natural ventilation open-sided conventional house as the most appropriate rearing system

关键词： AHP Energy use Litter Noxious gasses Poultry production Ventilation system

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New patterns of the tree beta diversity and its determinants in the largest savanna and wetland biomes of South America

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Plant Diversity 2023年第4期45卷 369-384页

作者： karla j.P.Silva-Souza Maíra G.Pivato Vinícius C.Silva Ricardo F.Haidar Alexandre F.Souza Departamento de Ecologia Universidade Federal do Rio Grande do NorteRNBrazil Curso de Engenharia Ambiental Universidade Federal do TocantinsTOBrazil

Clear and data-driven bioregionalizations can provide a framework to test hypotheses and base biodiversity *** we used occurrence and abundance data in combination with objective analytical methods to propose two bioregionalization schemes for tree species of the Cerrado and the Pantanal in South *** also evaluated the contribution of three sets of determinants of the occurrence-and abundance-based *** compiled data on tree species composition from 894 local assemblages based on species occurrences,and from 658 local assemblages based on species *** used an unconstrained community-level modelling approach and clustering techniques to identify and map tree subregions for the occurrence and the abundance data sets,*** clustering analyses were conducted to investigate floristic affinities between the subregions and to map broader floristic *** used multinomial logistic regression models,deviance partitioning,and rank-sum tests to assess the main subregion *** identified 18 occurrence-and four abundance-based subregions in the *** hierarchical classifications grouped the occurrence-based subregions into nine floristic zones and abundance-based subregions into two broad floristic *** in subregions were explained mainly by environmental factors and spatial structure in both occurrence and abundance data *** occurrence-and abundance-based subregions are complementary approaches to disentangle macroecological patterns and to plan conservation efforts in the Cerrado and the *** findings based on occurrence data revealed more complex and interdigitated boundaries between subregions of tree species than previously *** environment,historical stability,and human effects act in a synergetic way on the distribution of the ***,the relevance of contemporary environmental factors to the subregion patterns we found alert us to the profound im

关键词： Cerrado Pantanal Savanna Biogeographical regionalization Biodiversity hotspot South America

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Cyclophosphamide-associated enteritis: A rare association with severe enteritis

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World journal of Gastroenterology 2016年第39期22卷 8844-8848页

作者： Linda S Yang karla Cameron Tim Papaluca Chamara Basnayake Louise jackett Penelope McKelvie David Goodman Barbara Demediuk Sally j Bell Alexander j Thompson Department of Gastroenterology St. Vincent’s HospitalFitzroy Department of Pathology St. Vincent’s HospitalFitzroy Department of Nephrology St. Vincent’s HospitalFitzroy

Cyclophosphamide is a potent cytotoxic agent used in many clinical settings. The main risks of cyclophosphamide therapy include hematological disorders, infertility, hemorrhagic cystitis and malignancies. Gastrointestinal side effects reported to date are often non-specific and not severe. We present the first case of a fatal small bowel enteritis and pan-colitis which appears to be associated with cyclophosphamide. We aim to raise the readers' awareness of this significant adverse event to facilitate clinical suspicion and early recognition in potential future cases.

关键词： Enteritis Colitis Cyclophosphamide Small bowel Pan-colitis

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No independent associations between physical activity and clinical outcomes among hospitalized patients with moderate to severe COVID-19

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journal of Sport and Health Science 2021年第6期10卷 690-696页

作者： Ana j.Pinto karla F.Goessler Alan L.Fernandes Igor H.Murai Lucas P.Sales Bruna Z.Reis Mayara Diniz Santos Hamilton Roschel Rosa M.R.Pereira Bruno Gualano Applied Physiology and Nutrition Research Group School of Physical Education and SportClinical Hospital HCFMUSPSchool of Medicine FMUSPUniversity of Sao PauloSao PauloSP 01246903Brazil Rheumatology Division Clinical Hospital HCFMUSPSchool of Medicine FMUSPUniversity of Sao PauloSao PauloSP 01246903Brazil Food Research Center University of Sao PauloSao PauloSP 05508080Brazil

Background:Regular physical activity(PA)has been postulated to improve,or at least maintain,immunity across the life ***,the link between physical(in)activity and coronavirus disease 2019(COVID-19)remains to be *** small-scale prospective cohort study is nested within a randomized controlled trial aimed to investigate the possible associations between PA levels and clinical outcomes among hospitalized patients with moderate to severe ***:Hospitalized patients with COVID-19(mean age:54.9 years)were recruited from the Clinical Hospital of the School of Medicine of the University of Sao Paulo(a quaternary referral teaching hospital)and from Ibirapuera Field Hospital,both located in Sao Paulo,*** level was assessed using the Baecke Questionnaire of Habitual Physical *** primary outcome was hospital length of *** secondary outcomes were mortality,admission to the intensive care unit(ICU),and mechanical ventilation ***:The median hospital length of stay was 7.0§4.0 days,median§IQR;3.3%of patients died,13.8%were admitted to the ICU,and 8.6%required mechanical *** linear regression models showed that PA indices were not associated with hospital length of stay(work index:b=-0.57(95%confidence interval(95%CI):-1.80 to 0.65),p=0.355;sport index:b=0.43(95%CI:-0.94 to 1.80),p=0.536;leisure-time index:b=1.18(95%CI:-0.22 to 2.59),p=0.099;and total activity index:b=0.20(95%CI:-0.48 to 0.87),p=0.563).None of the PA indices were associated with mortality,admission to the ICU,or mechanical ventilation requirement(all p>0.050).Conclusion:Among hospitalized patients with COVID-19,PA did not independently associate with hospital length of stay or any other clinically relevant *** findings should be interpreted as meaning that,among already hospitalized patients with more severe forms of COVID-19,being active is a potential protective factor likely outweighed by a cluster of comorbidities(e.

关键词： Hospital length of stay Lifestyle Physical inactivity Prognosis SARS-CoV-2

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Spatial distribution and network morphology of epicardial,endocardial,interstitial,and Purkinje cell-associated elastin fibers in porcine left ventricle

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Bioactive Materials 2023年第1期19卷 348-359页

作者： Xiaodan Shi Song Zhang Yue Liu Bryn Brazile jim Cooley j.Ryan Butler Sara R.McMahan karla L.Perez jiazhu Xu Timothy Eastep Kytai T.Nguyen Pietro Bajona Matthias Peltz Huajian Gao Yi Hong jun Liao Department of Bioengineering University of Texas at ArlingtonArlingtonTX76010USA College of Engineering and College of Veterinary Medicine Mississippi State UniversityMississippi StateMS39762USA School of Engineering Brown UniversityProvidenceRI02912USA College of Engineering Nanyang Technical University308232Singapore Department of Cardiovascular and Thoracic Surgery University of Texas Southwestern Medical CenterDallasTX75390USA Allegheny Health Network-Drexel University College of Medicine PittsburghPA15212USA

Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac *** studies have mainly focused on collagen,the major structural ECM in heart *** role of elastin in cardiac mechanics,however,is poorly *** this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left *** demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber *** compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface *** myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte *** collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during ***,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in *** short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.

关键词： Heart ECM Epicardial elastin Endocardial elastin Interstitial elastin Purkinje-cell associated elastin

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A New Nano-Platform for Drug Release via Nanotubular Aluminum Oxide

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journal of Biomaterials and Nanobiotechnology 2011年第3期2卷 226-233页

作者： Kunbae Noh karla S. Brammer Chulmin Choi Seung Hyun Kim Christine j. Frandsen Sungho jin 不详

Nanotubular materials have many favorable properties for drug delivery. We present here a pioneering study of controlled release of a model drug, amoxicillin, from the internal nanopore structure of self-ordered, periodically spaced-apart aluminum oxide with an innovative, nanotubular geometry. This aluminum oxide nanotube geometry has not yet been revealed for biological applications, thus we have selected this oxide nanotube structure and demonstrated its ability as a drug carrier. Controlled, sustained release was achieved for over 5 weeks. The release kinetics from the nanotube layer was thoroughly characterized and it was determined that the amount of drug released was proportional to the square root of time. This type of controlled release and longevity from the nanotube layer has potential for therapeutic surface coatings on medical implants. Furthermore, this type of geometry has many features that are advantageous and biologically relevant for enhancing tissue biointegration.

关键词： AAO nanotube antibiotics drug release implant surface

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