Spatial distribution and network morphology of epicardial,endocardial,interstitial,and Purkinje cell-associated elastin fibers in porcine left ventricle

作     者：Xiaodan Shi Song Zhang Yue Liu Bryn Brazile Jim Cooley J.Ryan Butler Sara R.McMahan Karla L.Perez Jiazhu Xu Timothy Eastep Kytai T.Nguyen Pietro Bajona Matthias Peltz Huajian Gao Yi Hong Jun Liao 

作者机构：Department of BioengineeringUniversity of Texas at ArlingtonArlingtonTX76010USA College of Engineering and College of Veterinary MedicineMississippi State UniversityMississippi StateMS39762USA School of EngineeringBrown UniversityProvidenceRI02912USA College of EngineeringNanyang Technical University308232Singapore Department of Cardiovascular and Thoracic SurgeryUniversity of Texas Southwestern Medical CenterDallasTX75390USA Allegheny Health Network-Drexel University College of MedicinePittsburghPA15212USA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第19卷第1期

页      面：348-359页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：support:R01EB022018 R15HL140503 T32 HL134613 from NIH UT STARS 

主　　题：Heart ECM Epicardial elastin Endocardial elastin Interstitial elastin Purkinje-cell associated elastin 

摘      要：Cardiac extracellular matrices(ECM)play crucial functional roles in cardiac *** studies have mainly focused on collagen,the major structural ECM in heart *** role of elastin in cardiac mechanics,however,is poorly *** this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left *** demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber *** compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface *** myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte *** collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during ***,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECM association could be essential in organizing cardiac Purkinje cells into“fibrousand“branchingmorphologies and serving as a protective feature when Purkinje fibers experience large deformations in *** short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECM component.