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Period-Doubling Cascades and Strange Attractors in Extended Duffing-Van der Pol Oscillator

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Communications in Theoretical Physics 2009年第5期51卷 865-868页

作者： yu jun PAN wei-Zhen ZHANG Rong-Bo Institute of Nonlinear Science Shaoxing University Shaoxing 312000 China

The dynamical behavior of the extended Duffing-Van der Pol oscillator is investigated numerically in detail. With the aid of some numerical simulation tools such as bifurcation diagrams and Poinearé maps, the different routes to chaos and various shapes of strange attractors are observed. To characterize chaotic behavior of this oscillator system, the spectrum of Lyapunov exponent and Lyapunov dimension are also employed.

关键词： extended Duffing-Van der Pol oscillator bifurcation chaos

Pulsating magneto-dipole radiation of a quaking neutron star powered by energy of Alfvn seismic vibrations

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Research in Astronomy and Astrophysics 2011年第9期11卷 1085-1092页

作者： Sergey Bastrukov jun-wei yu Ren-Xin Xu Irina Molodtsova State Key Laboratory of Nuclear Physics and Technology School of Physics Peking University Beijing 100871 China Joint Institute for Nuclear Research 141980 Dubna Russia

We compute the characteristic parameters of the magneto-dipole radiation of a neutron star undergoing torsional seismic vibrations under the action of Lorentz restoring force about an axis of a dipolar magnetic field experiencing decay. After a brief outline of the general theoretical background of the model of a vibration-powered neutron star, we present numerical estimates of basic vibration and radiation characteristics, such as frequency, lifetime and luminosity, and investigate their time dependence on magnetic field decay. The presented analysis suggests that a gradual decrease in frequencies of pulsating high-energy emission detected from a handful of currently monitored AXP/SGR-like X-ray sources can be explained as being produced by the vibration-powered magneto-dipole radiation of quaking magnetars.

关键词： neutron stars -- torsion Alfv6n vibrations -- vibration powered radiation -- magnetic field decay -- magnetars

Effect of mutant p27^(kip1) gene on human cholangiocarcinoma cell line, QBC_(939)

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World Journal of Gastroenterology 2008年第34期14卷 5344-5348页

作者： Jian Luo Yong-jun Chen wei-yu Wang Sheng-Quan Zou Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM：To investigate the effects of exogenously mutated p27^kip1 （p27） on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in ***： Adenviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow ***： The expression of p27 protein and mRNA was increased signifi cantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could signifi cantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with ***： p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.

关键词： Adenovirus Cholangiocarcinoma Gene therapy Cell cycle Apoptosis

Metabolomic Study on Vitamins B_1, B_2, and PP Supplementation to Improve Serum Metabolic Profiles in Mice under Acute Hypoxia Based on ~1H NMR Analysis

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Biomedical and Environmental Sciences 2010年第4期23卷 312-318页

作者： JIN LIU JIAN-QUAN WU JI-jun YANG JING-yu wei wei-NA GAO CHANG-JIANG GUO Institute of Hygiene and Environmental Medicine Tianjin 300050 China

Objective To explore metabolic changes after acute hypoxia and modulating effect of vitamins B1, B2, and PP supplementation in mice exposed to acute hypoxia. Methods Fifty male Kunming mice were randomly divided into 5 groups： normal, acute hypoxia, acute hypoxia with 2, 4 and 8 time- vitamins B1, B2, and PP supplementation . All mice were fed with corresponding diets for two weeks and then were exposed to a simulated altitude of 6 000 meters for 8 h, except for the normal group. Nuclear magnetic resonance analysis was used to identify the changes of serum metabolic profiles. Results There were significant changes in some serum metabolites under induced acute hypoxia, essentially relative increase in the concentrations of lactate, sugar and lipids and decrease in ethanol. The serum levels of choline, succinate, taurine, alanine, and glutamine also increased and phosphocholine decreased in the acute hypoxia group. After vitamins B1, B2, and PP supplementation, all these metabolic changes gradually recovered. Conclusion Significant changes in serum metabolic profile were observed by metabolomics in mice exposed to acute hypoxia, and vitamins B1, B2, and PP supplementation proved to be beneficial to improving some metabolic pathways. It is suggested that the dietary intakes of vitamins B1, B2, and PP should be increased under hypoxia condition.

关键词： Vitamins B1 B2 and PP Acute hypoxia 1H NMR Mice

Insulin-like signaling pathway functions in integrative response to an olfactory and a gustatory stimuli in Caenorhabditis elegans

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Protein & Cell 2010年第1期1卷 75-81页

作者： Ya-Ming Jiu Yang yue Song Yang Lin Liu jun-wei yu Zheng-Xing Wu Tao Xu Key Laboratory of Molecular Biophysics Ministry of Educationand Institute of Biophysics and BiochemistryHuazhong University of Science and TechnologyWuhan 430074China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Animals integrate various environmental stimuli within the nervous system to generate proper behavioral ***,the underlying neural circuits and molecular mechanisms are largely *** insulinlike signaling pathway is known to regulate dauer formation,fat metabolism,and longevity in Caenorhabditis elegans(***).Here,we show that this highly conserved signaling pathway also functions in the integrative response to an olfactory diacetyl and a gustatory Cu^(2+) *** of wild-type N2 Bristol displayed a strong avoidance to the Cu^(2+) barrier in the migration pathway to the attractive *** of daf-2(insulin receptor),daf-18(PTEN lipid phosphatase),pdk-1(phosphoinositide-dependent kinase),akt-1/-2(Akt/PKB kinase)and sgk-1(serum-and glucocorticoidinducible kinase)show severe defects in the elusion from the Cu^(2+) .Mutations in DAF-16,a forkhead-type transcriptional factor,suppress the integrative defects of daf-2 and akt-1/-2 *** further report that neither cGMP nor TGFβpathways,two other dauer formation regulators,likely plays a role in the integrative *** results suggest that the insulin-like signaling pathway constitutes an essential component for sensory integration and decision-making behavior plasticity.

关键词： C.elegans insulin signaling pathway sensory integration decision making behavior

Preparation and Identification of Monoclonal Antibodies against Chicken Immunoglobulin G

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Animal Husbandry and Feed Science 2010年第10期2卷 26-27页

作者： WANG jun-jun yu wei-yi Key Laboratory of Zoonoses of Anhui Province Anhui Agricultural University Hefei 230036 China

[ Objective] To prepare monoclonal antibodies against chicken immunoglobulin G （IgG） and improve the diagnostic level of specific antibodies in chickens. [ Method] Chicken IgG was isolated by saturated ammonium sulfate and purified by Sephadex G-200 column chromatography. Then the BALB/c mice were immunized by the chicken IgG, and the spleen cells were fused with mouse myeloma cells SP2/0. Finally, the positive hybridoma cells were screened and detected by indirect enzyme-linked immunosorbent assay （ELISA）. [ Result] Four hybridoma cell strains secre- ting monoclonal antibodies against chicken IgG were obtained and named as C44, C45, C67 and C68, and their ascites titers in indirect ELISA were 1 ： 640 000, 1 ： 320 000, 1 ： 640 000 and 1 ： 80 000, respectively. The monoclonal antibodies secreted by C44 and C45 could recognize light chains of chicken IgG and those secreted by C,67 and C68 could recognize heavy chains of chicken IgG. They all could not recognize IgG from duck, rabbit and swine. Additionally, the Ig type identification results showed that they all belonged to IgGl. [ Conclusion] Four cell strains of obtained hybridoma can stably produce the monoclonal antibodies against chicken IgG.

关键词： Chicken IgG Monoclonal antibody. SPecificity. Identification

A new exploration on the creation of grafted breast cancer model for MA891 cells in TA2 mice

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Chinese Medical Journal 2005年第21期118卷 1830-1833页

作者： GU jun-chao yu wei-bo ZHANG Zhong-tao WANG yu Department of General Surgery Beijing Friendship Hospital Affiliated with Capital University of Medical Sciences Beijing 100050 China

Animal experimental systems are particularly useful for the study of human breast cancer. An ideal model shoulcl be easy to use, closely mimicking human physiopathology and has a stable tumor morbidity. The cell line MA891 was established from a spontaneous TA2 mouse mammary carcinoma by Cancer Institute of Chinese Academy of Medical Sciences. 3 Some researches indicated that MA891 had a very low immunogenecity and maintained a high metastatic potential in vivo. So it has been used as a better grafted mouse tumor model for studying cancer physiopathology and metastasis in human for years. However, about the biological characteristic and the histopathologic feature of this model there has been a lack of investigations.

关键词： breast cancer MA891 cell animal model

Hypobaric Hypoxia Aggravates Renal Injury by Inducing the Formation of Neutrophil Extracellular Traps through the NF-κB Signaling Pathway

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Current Medical Science 2023年第3期43卷 469-477页

作者： jun-yu wei Miao-yue HU Xiu-qi CHEN Jin-shuang wei Jie CHEN Xuan-kai QIN Feng-ying LEI Jia-sen ZOU Shi-qun ZHU yuan-han QIN Department of Pediatrics the First Affiliated HospitalGuangxi Medical UniversityNanning 530021China Children's Hospital of Chongqing Medical University Chongqing 400015China Shenzhen Children's Hospital Shenzhen 518034China

Objective The hypersensitivity of the kidney makes it susceptible to hypoxia *** involvement of neutrophil extracellular traps(NETs)in renal injury resulting from hypobaric hypoxia(HH)has not been *** this study,we aimed to investigate the expression of NETs in renal injury induced by HH and the possible underlying *** A total of 24 SD male rats were divided into three groups(n=8 each):normal control group,hypoxia group and hypoxia+pyrrolidine dithiocarbamate(PDTC)*** in hypoxia group and hypoxia+PDTC group were placed in animal chambers with HH which was caused by simulating the altitude at 7000 meters(oxygen partial pressure about 6.9 kPa)for 7 *** was administered at a dose of 100 mg/kg intraperitoneally once daily for 7 *** changes of the rat renal tissues were observed under a light microscope;the levels of serum creatinine(SCr),blood urea nitrogen(BUN),cell-free DNA(cf-DNA)and reactive oxygen species(ROS)were measured;the expression levels of myeloperoxidase(MPO),citrullinated histone H3(cit-H3),B-cell lymphoma 2(Bcl-2),Bax,nuclear factor kappa B(NF-κB)p65 and phospho-NF-κB p65(p-NF-κB p65)in rat renal tissues were detected by qRT-qPCR and Western blotting;the localization of NF-κB p65 expression in rat renal tissues was observed by immunofluorescence staining and the expression changes of NETs in rat renal tissues were detected by multiplex fluorescence immunohistochemical *** After hypoxia,the expression of NF-κB protein in renal tissues was significantly increased,the levels of SCr,BUN,cf-DNA and ROS in serum were significantly increased,the formation of NETs in renal tissues was significantly increased,and a large number of tubular dilatation and lymphocyte infiltration were observed in renal *** PDTC was used to inhibit NF-κB activation,NETs formation in renal tissue was significantly decreased,the expression level of Bcl-2 in renal tissues was significantly increased,the expres

关键词： hypobaric hypoxia neutrophil extracellular traps hypoxia kidney injury NF-κB apoptosis

Analysis of professor Wang Yongyan's experience in treating hemorrhagic diseases based on traditional Chinese medicine inheritance support system

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TMR Theory and Hypothesis 2018年第3期1卷 63-71页

作者： jun-yu wei Ze-Wang Liu Ling-yu Qi Chao Zhou Department of Oncology Weifang Traditional Chinese HospitalWeifangChina Yantaishan Hospital YantaiChina College of First Clinical Medicine Shandong University of Traditional Chinese MedicineJinan 250014ShandongChina

Based on the data analysis platform provided by TCMISS(V2.5),this paper analyzes the rule of prescribing drugs used by Professor Yongyan Wang for the treatment of apoplexy and deepens the understanding of his academic research on syndrome differentiation and *** Wang’s medical records were summarized and sorted into the"Chinese Medicine inheritance Assistance system".Through the functions of"frequency statistics","drug use mode","rule analysis"and so on,the characteristics of professor Yongyan Wang's prescription for treating apoplexy were statistically *** is helpful to understand and inherit Professor Wang's academic thoughts by analyzing the data of professor Wang's medical records of apoplexy.

关键词： Apoplexy Syndrome differentiation TCM inheritance platform