Metabolomic Study on Vitamins B_1, B_2, and PP Supplementation to Improve Serum Metabolic Profiles in Mice under Acute Hypoxia Based on ~1H NMR Analysis

作     者：JIN LIU JIAN-QUAN WU JI-JUN YANG JING-YU WEI WEI-NA GAO CHANG-JIANG GUO 

作者机构：Institute of Hygiene and Environmental Medicine Tianjin 300050 China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2010年第23卷第4期

页      面：312-318页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0905[农学-畜牧学] 09[农学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by a grant from the National Natural Science Foundation of China (NO. 30872097) 

主　　题：Vitamins B1 B2 and PP Acute hypoxia 1H NMR Mice 

摘      要：Objective To explore metabolic changes after acute hypoxia and modulating effect of vitamins B1, B2, and PP supplementation in mice exposed to acute hypoxia. Methods Fifty male Kunming mice were randomly divided into 5 groups： normal, acute hypoxia, acute hypoxia with 2, 4 and 8 time- vitamins B1, B2, and PP supplementation . All mice were fed with corresponding diets for two weeks and then were exposed to a simulated altitude of 6 000 meters for 8 h, except for the normal group. Nuclear magnetic resonance analysis was used to identify the changes of serum metabolic profiles. Results There were significant changes in some serum metabolites under induced acute hypoxia, essentially relative increase in the concentrations of lactate, sugar and lipids and decrease in ethanol. The serum levels of choline, succinate, taurine, alanine, and glutamine also increased and phosphocholine decreased in the acute hypoxia group. After vitamins B1, B2, and PP supplementation, all these metabolic changes gradually recovered. Conclusion Significant changes in serum metabolic profile were observed by metabolomics in mice exposed to acute hypoxia, and vitamins B1, B2, and PP supplementation proved to be beneficial to improving some metabolic pathways. It is suggested that the dietary intakes of vitamins B1, B2, and PP should be increased under hypoxia condition.