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Influence of the concentration of dietary digestible calcium on growth performance,bone mineralization, plasma calcium, and abundance of genes involved in intestinal absorption of calcium in pigs from 11 to 22 kg fed diets with different concentrations of

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Journal of Animal Science and Biotechnology 2019年第4期10卷 942-957页

作者： l.Vanessa lagos Su A.lee Guillermo Fondevila Carrie l.Walk Michael R.Murphy juan J.loor Hans H.Stein Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA Department of Animal Sciences University of IllinoisUrbanaIL 61801USA Universidad Politécnica de Madrid MadridSpain AB Vista Marlborough SN84ANUK

Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible(STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of genes related to Ca absorption(TRPV6, S100 G, and ATP2 B1) in the duodenum, and tight junction proteins(OClN, ClDN1, and ZO1) in the duodenum and ***: Twenty corn-soybean meal diets were formulated using a 4 × 5 factorial design with diets containing 0.16%, 0.33%, 0.42%, or 0.50% STTD P, and 0.14%, 0.29%, 0.44%, 0.59%, or 0.74% STTD Ca. Six hundred and forty pigs(initial weight: 11.1 ± 1.4 kg) were allotted to 20 diets and 5 blocks in a randomized complete block design. On day21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of m RNA was determined in duodenal and ileal tissue via quantitative RT-PCR. Data were analyzed using a response surface ***: The predicted maximum ADG(614 g), G:F(0.65), and bone ash(11.68 g) was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively, when STTD P was provided at the requirement(0.33%). If dietary STTD P was below the requirement, increasing dietary Ca resulted in reduced(P ly if dietary STTD Ca exceeded 0.6%. Plasma Ca concentration was positively affected by STTD Ca over the range studied(quadratic, P ly affected by increasing STTD P(linear, P linear negative effect(P lN, and ZO1 in duodenum, and ClDN and ZO1 in ***: The STTD Ca:STTD P ratio needed to maximize growth performanc

关键词： Bone ash Calcium absorption Digestible calcium Growth Pigs Requirement

Complex patterns of precipitation and extreme events during 1951-2011 in Sichuan Basin, Southwestern China

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Journal of Mountain Science 2018年第2期15卷 340-356页

作者： ZHOU Xiang-yang lEI Wen-juan Guizhou Provincial Key laboratory of Public Big Data Guizhou University State Key laboratory of Hydraulics and Mountain River Engineering Sichuan University College of Architecture & Environment Sichuan University

Sichuan Basin is located in southwestern China and affected by a complex water vapor （WV） sources. Here, the spatial and temporal patterns of precipitation and extreme events are investigated by six indices of World Meteorology Organization Commission, including annual precipitation total （AP）, maximum daily precipitation （Maxld）, intensity of rainfall over 1 mm/d （IR1）, maximum and mean consecutive dry days （Max CDD, Mean CDD） and coefficient of variance. Based on 24 daily precipitation time series from 1951 to 2o11, Mann-Kendall test is employed to quantify the significant level of these indices, from which the classification of precipitation change and its spatial patterns are obtained. Meanwhile, the probability distributions of these indices are identified by l-moment analysis and the Goodness-of-fit test, and the corresponding values are calculated by theoretical model at different return periods. The results reveal that the western basin displays normal drought： less AP and precipitation intensity while longer drought. The southern basin shows normal increase： larger AP and precipitation intensity but shorter CDD. However, in hilly region of the central basin and the transition zone between basin and mountains, precipitation changes abnormally： increasing both drought （one or both of Mean CDD and MaxCDD） and precipitation intensity （one or both of Maxld and trend of AP is. Probability IR1） no matter what the distribution models also demonstrate the complex patterns： a negative correlation between Maxld and Max CDD in the west （R2≥0.61） while a positive correlation in the east （R2≥0.41） at all return periods. These patterns are induced by the changes in WV sources and the layout of local terrain. The increase of WV in summer and decrease in spring leads to the heavier rainfall and longer drought respectively. The large heat island effect of the basin contributes to a lower temperature in transition zones and more precipitation in the downwind area. These resul

关键词： Extreme precipitation events Climatechange Sichuan Basin Trend analysis Probability distribution

Occurrence and behavior of pharmaceuticals in sewage treatment plants in eastern China

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Frontiers of Environmental Science & Engineering 2015年第4期9卷 725-730页

作者： juan DU Yu FAN Xin QIAN The State Key laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China Xuzhou Research Institute of Environmental Xuzhou 221002 China

The occurrence and removal efficiency of seven pharmaceuticals （norfloxacin, trimethoprim, roxithromycin, sulfamethoxazole, ibuprofen, diclofenac and carbamazepine） were determined in three sewage treatment plants （STPs） with anaerobic/anoxic/oxic, anoxic/oxic and oxidation ditches processes in Xuzhou City, Eastern China. The results showed that seven pharmaceuticals were detected in the influent samples with concentrations ranging from 93 to 2540 ng·l^-1. The removal of these substances among the three different STPs varied from 36 to 84%, with the highest performance obtained by the wastewater treatment works with tertiary treatment （sand filtration）. Most of the compounds were removed effectively during biologic treatment while sand filtration treatment also made a contribution to the total elimination of most pharmaceuticals. The efficiency comparison of the three sewage treatment processes showed that the STP which employed anaerobic/anoxic/oxic was more effective to remove pharmaceuticals than the oxidation ditches and anoxic/oxic.

关键词： pharmaceutical anaerobic/anoxie/oxic oxidation ditch anoxic/oxic removal efficiency

Preparation of sulfur hydrophobized plasmonic photocatalyst towards durable superhydrophobic coating material

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Journal of Materials Science & Technology 2020年第6期41卷 159-167页

作者： Emese lantos lászlóMérai ágota Deák juan Gómez-Pérez Dániel Sebo?k Imre Dékány Zoltán Kónya lászlóJanovák University of Szeged Interdisciplinary Excellence CentreDepartment of Physical Chemistry and Materials ScienceH-6720Rerrich Béla tér 1SzegedHungary University of Szeged Interdisciplinary Excellence CentreDepartment of Applied and Environmental ChemistryH-6720Rerrich Béla tér 1SzegedHungary

The widely used photocatalytic self-cleaning coating materials are often made of polymers and polymer based composites,where the photocatalyst immobilization occurs with ***,these organic polymers are often unstable under exposure to UV irradiation and easily degraded by reactive radicals produced in the photocatalytic *** order to solve this problem,in this paper,we present the facile preparation of a multifunctional coating with dual superhydrophobic and photocatalytic properties,where the fixation and the hydrophobization of the plasmonic Ag-TiO2 photocatalyst particles with visible light activity was performed with non-water soluble sulfur,which is a cheap and easily available *** resulted novel nanocomposite with rough and nano-tructured surface roughness(1.25–2.45 nm determined by small-angle X-ray scattering)has sufficient low surface energy(3.3 mJ/m2)for superhydrophobic(θ=151.1°v)***,in contrast of the organic and expensive fluoropolymer based composites,this non-wetting nature was durable,because the measured was higher than 150°during the long-term lED(λmax=405 nm)light irradiation.

关键词： Nanocomposite Coating materials Wetting lotus-type structure Visible light photocatalyst

light induces petal color change in Quisqualis indica(Combretaceae)

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Plant Diversity 2018年第1期40卷 28-34页

作者： juan Yan Menglin Wang ling Zhang Key laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnan 666303China University of Chinese Academy of Sciences Beijing 100049China

Petal color change, a common phenomenon in angiosperms, is induced by various environmental and endogenous factors. Interestingly, this phenomenon is important for attracting pollinators and further reproductive success. Quisqualis indica l.(Combretaceae) is a tropical Asian climber that undergoes sequential petal color change from white to pink to red. This color changing process is thought to be a good strategy to attract more pollinators. However, the underlying physiological and biochemical mechanisms driving this petal color change phenomenon is still underexplored. In this context, we investigated whether changes in pH, pollination, light, temperature or ethylene mediate petal color change. We found that the detected changes in petal pH were not significant enough to induce color alterations. Additionally, pollination and temperatures of 20-30℃did not alter the rate of petal color change; however, flowers did not open when exposed to constant temperatures at 15℃ or 35℃.Moreover, the application of ethylene inhibitor, i.e., silver thiosulphate, did not prevent color change. It is worth mentioning here that in our study we found light as a strong factor influencing the whole process of petal color change, as petals remained white under dark conditions. Altogether, the present study suggests that petal color change in Q. indica is induced by light and not by changes in petal pH, pollination, ethylene, or temperature, while extremely low or high temperatures affect flower anthesis. In summary, our findings represent the probable mechanism underlying the phenomenon of petal color change, which is important for understanding flower color evolution.

关键词： Environmental factors Ethylene Petal color pH Pollination Temperature

GIS-based study of the evolution of the IGN's strong motion network of mainland Spain and the balearics

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Geodesy and Geodynamics 2021年第4期12卷 279-287页

作者： Jose-lazaro Amaro-Mellado Daniel Anton juan-Manuel Alcalde Instituto Geografico Nacional Servicio Regional en AndaluciaSeville 41013Spain Departamento de Ingenierfa Grafica Universidad de SevillaSeville 41092Spain Research Group TEP970:Innovacion Tecnoldgica Sistemas de Modelado 3D y Diagnosis Energetica en Patrimonio y Edificacidn'Departamento de Expresion Grafica e Ingenieria en la EdificacionEscuela Tecnica Superior de Ingenieria de EdificacionUniversidad de Sevilla4A Reina Mercedes AvenueSeville 41012Spain The Creative and Virtual Technologies Research laboratory School of ArchitectureDesign and the Built EnvironmentNottingham Trent University50 Shakespeare StreetNottingham NG14FQUK Escuela Superior de Ingenieria y Tecnologia Universidad Intemacional de La Rioja737 La Paz AvenueLogrono 26006Spain Instituto Geografico Nacional Red Sismica NacionalMadrid 28003Spain

In some countries with seismicity,an adequate detection network is essential to assess seismic *** Instituto Geografico Nacional(National Geographic Institute/IGN)of Spain manages a strong motion network distributed throughout the whole country and an earthquake detection network composed of velocity *** paper aims at studying the development of the strong motion network of the Spanish National Seismic Network(focusing on mainland Spain and the Balearics)in the last three decades.A Geographic Information System(GIS)has been implemented to integrate the attributes from the IGN’s database concerning the earthquake catalogue,seismograph and accelerometer networks,which enabled to analyse the data and map the distribution of detection equipments in the *** irregularly distributed Spanish strong motion network was greatly expanded from 1990 to *** the past decade,it developed at a slower rate,but more modern technology was implemented.

关键词： Strong motion Accelerometer network Spanish national seismic network Earthquake catalogue GIS

The Influence of Co-Suppressing Tomato 1-Aminocyclopropane-1-Carboxylic Acid OxidaseⅠon the Expression of Fruit Ripening-Related and Pathogenesis-Related Protein Genes

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Agricultural Sciences in China 2007年第4期6卷 406-413页

作者： HU Zong-li CHEN Xu-qing CHEN Guo-ping lü li-juan Grierson Donald College of Bioengineering Chongqing University Chongqing 400030 P.R.China Plant Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough Leicestershire LE12 5RD UK

The purpose of this study is to explore the influence of co-suppressing tomato ACC oxidase Ⅰ on the expression of fruit ripening-related and pathogenesis-related protein genes, and on the biosynthesis of endogenous ethylene and storage ability of fruits. Specific fragments of several fruit ripening-related and pathogenesis-related protein genes from tomato （lycopersicon esculentum） were cloned, such as the l-aminocyclopropane-1-carboxylic acid oxidase 1 gene （leAC01）, 1- aminocyclopropane-l-carboxylic acid oxidase 3 gene （leAC03）, EIN3-binding F-box 1 gene （leEBF1）, pathogenesis-related protein 1 gene （lePR1）, pathogenesis-related protein 5 gene （lePR5）, and pathogenesis-related protein osmotin precursor gene （leNP24） by PCR or RT-PCR. Then these specific DNA fragments were used as probes to hybridize with the total RNAs extracted from the wild type tomato Ailsa Craig （AC＋＋） and the leAC01 co-suppression tomatoes （V1187 and T4B）, respectively. At the same time, ethylene production measurement and storage experiment of tomato fruits were carded out. The hybridization results indicated that the expression of fruit ripening-related genes such as leACO3 and leEBF1, and pathogenesis-related protein genes such as lePR1, lePR5, and leNP24, were reduced sharply, and the ethylene production in the fruits, wounded leaves decreased and the storage time of ripening fruits was prolonged, when the expression of leACO1 gene in the transgenic tomato was suppressed. In the co-suppression tomatoes, the expression of fruit ripening-related and pathogenesis-related protein genes were restrained at different degrees, the biosynthesis of endogenous ethylene decreased and the storage ability of tomato fruits increased.

关键词： co-suppression leACO1 fruit ripening pathogenesis-related protein genes

Interplay between HEAT SHOCK PROTEIN 90 and HY5 Controls PhANG Expression in Response to the GUN5 Plastid Signal

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Molecular Plant 2012年第4期5卷 901-913页

作者： Peter Kindgren louise Noren juan de Dios Barajas lopez Jehad Shaikhali Asa Strand Umea Plant Science Centre Department of Plant Physiology Umea University S-901 87 Umea Sweden

The presence of genes encoding organellar proteins in different cellular compartments necessitates a tight coordination of expression by the different genomes of the eukaryotic cell. This coordination of gene expression is achieved by organelle-to-nucleus or retrograde communication. Stress-induced perturbations of the tetrapyrrole pathway trigger large changes in nuclear gene expression in plants. Recently, we identified HSP90 proteins as ligands of the pu- tative plastid signal Mg-ProtolX. In order to investigate whether the interaction between HSP90 and Mg-ProtolX is bio- logically relevant, we produced transgenic lines with reduced levels of cytosolic HSP90 in wild-type and gun5 backgrounds. Our work reveals that HSP90 proteins respond to the tetrapyrrole-mediated plastid signal to control expres- sion of photosynthesis-associated nuclear genes （PhANG） during the response to oxidative stress. We also show that the hy5 mutant is insensitive to tetrapyrrole accumulation and that Mg-ProtolX, cytosolic HSP90, and HY5 are all part of the same signaling pathway. These findings suggest that a regulatory complex controlling gene expression that includes HSP90 proteins and a transcription factor that is modified by tetrapyrroles in response to changes in the environment is evolutionarily conserved between yeast and plants.

关键词： abiotic/environmental stress cell signaling organelle biogenesis/function.

Hypothalamic-pituitary-adrenal axis function during perinatal depression

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Neuroscience Bulletin 2015年第3期31卷 338-350页

作者： Phillipe leff Gelman Mónica Flores-Ramos Margarita lópez-Martínez Carlos Cruz Fuentes juan Pablo Reyes Grajeda National Institute of Perinatology Mexico City National Council of Sciences and Technology (CONACy T) Mexico City National Institute of Psychiatry Mexico City National Institute of Genomic Medicine Mexico City

Abnormal function of the hypothalamic-pituitary-adrenal （HPA） axis is an important pathological finding in pregnant women exhibiting major depressive disorder. They show high levels of cortisol pro- inflammatory cytokines, hypothalamic-pituitary peptide hormones and catecholamines, along with low dehydroepiandrosterone levels in plasma. During pregnancy, the TH2 balance together with the immune system and placental factors play crucial roles in the development of the fetal allograft to full term. These factors, when altered, may generate a persistent dysfunction of the HPA axis that may lead to an overt transfer of cortisol and toxicity to the fetus at the expense of reduced activity of placental 1113-hydroxysteroid dehydrogenase type 2. Epigenetic modifications also may contribute to the dysregulation of the HPA axis. Affective disorders in pregnant women should be taken seriously, and therapies focused on preventing the deleterious effects of stressors should be implemented to promote the welfare of both mother and baby.

关键词： brain depression neuroendocrine pregnancy stress glucocorticoids