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The Influence of Co-Suppressing Tomato 1-Aminocyclopropane-1-Carboxylic Acid OxidaseⅠon the Expression of Fruit Ripening-Related and Pathogenesis-Related Protein Genes

The Influence of Co-Suppressing Tomato 1-Aminocyclopropane-1-Carboxylic Acid OxidaseⅠon the Expression of Fruit Ripening-Related and Pathogenesis-Related Protein Genes

作     者:HU Zong-li CHEN Xu-qing CHEN Guo-ping Lü Li-juan Grierson Donald 

作者机构:College of Bioengineering Chongqing University Chongqing 400030 P.R.China Plant Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough Leicestershire LE12 5RD UK 

出 版 物:《Agricultural Sciences in China》 (中国农业科学(英文版))

年 卷 期:2007年第6卷第4期

页      面:406-413页

学科分类:09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基  金:supported by National Natural Science Foundation of China(30471180) Nature Science Foundation of Chongqing City,China(8045,2004-56) 

主  题:co-suppression LeACO1 fruit ripening pathogenesis-related protein genes 

摘      要:The purpose of this study is to explore the influence of co-suppressing tomato ACC oxidase Ⅰ on the expression of fruit ripening-related and pathogenesis-related protein genes, and on the biosynthesis of endogenous ethylene and storage ability of fruits. Specific fragments of several fruit ripening-related and pathogenesis-related protein genes from tomato (Lycopersicon esculentum) were cloned, such as the l-aminocyclopropane-1-carboxylic acid oxidase 1 gene (LeAC01), 1- aminocyclopropane-l-carboxylic acid oxidase 3 gene (LeAC03), EIN3-binding F-box 1 gene (LeEBF1), pathogenesis-related protein 1 gene (LePR1), pathogenesis-related protein 5 gene (LePR5), and pathogenesis-related protein osmotin precursor gene (LeNP24) by PCR or RT-PCR. Then these specific DNA fragments were used as probes to hybridize with the total RNAs extracted from the wild type tomato Ailsa Craig (AC++) and the LeAC01 co-suppression tomatoes (V1187 and T4B), respectively. At the same time, ethylene production measurement and storage experiment of tomato fruits were carded out. The hybridization results indicated that the expression of fruit ripening-related genes such as LeACO3 and LeEBF1, and pathogenesis-related protein genes such as LePR1, LePR5, and LeNP24, were reduced sharply, and the ethylene production in the fruits, wounded leaves decreased and the storage time of ripening fruits was prolonged, when the expression of LeACO1 gene in the transgenic tomato was suppressed. In the co-suppression tomatoes, the expression of fruit ripening-related and pathogenesis-related protein genes were restrained at different degrees, the biosynthesis of endogenous ethylene decreased and the storage ability of tomato fruits increased.

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