Influence of the concentration of dietary digestible calcium on growth performance,bone mineralization, plasma calcium, and abundance of genes involved in intestinal absorption of calcium in pigs from 11 to 22 kg fed diets with different concentrations of

作     者：L.Vanessa Lagos Su A.Lee Guillermo Fondevila Carrie L.Walk Michael R.Murphy Juan J.Loor Hans H.Stein 

作者机构：Division of Nutritional SciencesUniversity of IllinoisUrbanaIL 61801USA Department of Animal SciencesUniversity of IllinoisUrbanaIL 61801USA Universidad Politécnica de MadridMadridSpain AB VistaMarlborough SN84ANUK 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2019年第10卷第4期

页      面：942-957页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：Financial support for this research from AB Vista Marlborough UK 

主　　题：Bone ash Calcium absorption Digestible calcium Growth Pigs Requirement 

摘      要：Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible(STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of genes related to Ca absorption(TRPV6, S100 G, and ATP2 B1) in the duodenum, and tight junction proteins(OCLN, CLDN1, and ZO1) in the duodenum and ***: Twenty corn-soybean meal diets were formulated using a 4 × 5 factorial design with diets containing 0.16%, 0.33%, 0.42%, or 0.50% STTD P, and 0.14%, 0.29%, 0.44%, 0.59%, or 0.74% STTD Ca. Six hundred and forty pigs(initial weight: 11.1 ± 1.4 kg) were allotted to 20 diets and 5 blocks in a randomized complete block design. On day21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of m RNA was determined in duodenal and ileal tissue via quantitative RT-PCR. Data were analyzed using a response surface ***: The predicted maximum ADG(614 g), G:F(0.65), and bone ash(11.68 g) was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively, when STTD P was provided at the requirement(0.33%). If dietary STTD P was below the requirement, increasing dietary Ca resulted in reduced(P 0.05) ADG and G:F. However, if dietary STTD P was above the requirement, negative effects(P 0.05) on ADG and G:F of increasing STTD Ca were observed only if dietary STTD Ca exceeded 0.6%. Plasma Ca concentration was positively affected by STTD Ca over the range studied(quadratic, P 0.01) and negatively affected by increasing STTD P(linear, P 0.01). There was a linear negative effect(P 0.05) of STTD Ca on the abundance of S100 G, TRPV6, OCLN, and ZO1 in duodenum, and CLDN and ZO1 in ***: The STTD Ca:STTD P ratio needed to maximize growth performanc