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Effects of prostaglandin F_(2α) on small intestinal interstitial cells of Cajal

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World Journal of Gastroenterology 2011年第9期17卷 1143-1151页

作者： Chan Guk Park Young Dae Kim Man Yoo Kim Jae Woong Koh Jae Yeoul Jun Cheol Ho Yeum insuk so Seok Choi Department of Internal Medicine College of MedicineChosun University Department of Ophthalmology College of MedicineChosun University Department of Physiology College of MedicineChosun University Department of Physiology and Biophysics College of MedicineSeoul National University

AIM:To explore the role of prostaglandin F2α(PGF2α) on pacemaker activity in interstitial cells of Cajal(ICC)from mouse small intestine. METHODS:In this study,effects of PGF2αin the cultured ICC cells were investigated with patch clamp technology combined with Ca 2+ image analysis. RESULTS:Externally applied PGF2α(10μmol/L)produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp mode.The application of flufenamic acid(a non-selective cation channel inhibitor)or niflumic acid(aCl channel inhibitor)abolished the generation of pacemaker currents but only flufenamic acid inhibited the PGF2α-induced tonic inward currents.In addition,the tonic inward currents induced by PGF2αwere not inhibited by intracellular application of 5’-[-thio]diphosphate trilithium salt.Pretreatment with Ca 2+ free solution, U-73122,an active phospholipase C inhibitor,and thapsigargin,a Ca 2+ -ATPase inhibitor in endoplasmic reticulum,abolished the generation of pacemaker currents and suppressed the PGF2α-induced tonic inward currents.However,chelerythrine or calphostin C,protein kinase C inhibitors,did not block the PGF2α-induced effects on pacemaker currents.When recording intracellular Ca 2+ ([Ca 2+ ]i)concentration using fluo-3/AM,PGF2α broadly increased the spontaneous[Ca 2+ ]i oscillations. CONCLUSION:These results suggest that PGF2αcan modulate pacemaker activity of ICC by acting non-selective action channels through phospholipase C-dependent pathway via[Ca2+]i

关键词：间质细胞 ATP酶抑制剂小肠 fluo-3/AM 心脏起搏器细胞内钙诱导效应电流钳

Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity

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World Journal of Gastroenterology 2006年第5期12卷 796-799页

作者： Byung Joo Kim Jae Yeoul Jun insuk so Ki Whan Kim Department of Physiology and Biophysics College of MedicineSeoul National UniversityRepublic of Korea Department of Physiology College of MedicineChosun UniversityRepublic of Korea

瞄准：Cajal (国际计算中心) 的空隙的房间是产生慢波浪在的心律调整器房间胃肠(官方补给) 道。我们试图在 Cajal 的有教养的空隙的房间在肠的步调调整的活动调查 mitochondrial Na+-Ca2+ 交换的参与。方法：酶的消化被用来声言不赞成... 详细信息

瞄准：Cajal (国际计算中心) 的空隙的房间是产生慢波浪在的心律调整器房间胃肠(官方补给) 道。我们试图在 Cajal 的有教养的空隙的房间在肠的步调调整的活动调查 mitochondrial Na+-Ca2+ 交换的参与。方法：酶的消化被用来声言不赞成一只老鼠的小肠。整个房间的斑夹钳配置被用来记录膜电流(电压 clamp ) 和潜力(电流夹钳) 从有教养的国际计算中心。结果：Clonazepam 和 CGP37157 以一种剂量依赖者方式禁止了国际计算中心的步调调整的活动。从 20 ～ 60 micromol/L 的 Clonazepam 和从 10 ～ 30 micromol/L 的 CGP37157 有效地在国际计算中心的步调调整的活动从线粒体禁止了 Ca2+ 流出。clonazepam 和 CGP37157 的 IC50s 分别地是 37.1 和 18.2 micromol/L。到内部答案的 20 micromol/L NiCl2 的增加引起了一“打蜡”并且衰退国际计算中心的步调调整的活动的现象。结论：这些结果建议 mitochondrial Na+-Ca2+ 交换在肠的步调调整的活动有一个重要角色。

关键词：线立体肠疾病钠元素钙离子

Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

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World Journal of Gastroenterology 2013年第8期19卷 1210-1218页

作者： Dong Chuan Zuo Seok Choi Pawan Kumar Shahi Man Yoo Kim Chan Guk Park Young Dae Kim Jun Lee In Yeoup Chang insuk so Jae Yeoul Jun Department of Physiology College of MedicineChosun UniversityGwangju 501-759South Korea Department of Internal Medicine College of MedicineChosun UniversityGwangju 501-759South Korea Department of Anatomy College of MedicineChosun UniversityGwangju 501-759South Korea Department of Physiology and Biophysics College of MedicineSeoul National UniversitySeoul 56-1South Korea

AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small intestine.METHODS:For this study,primary culture of ICCs was prepared from the small intestine of the mouse.LPS was treated to the cells prior to measurement of the membrane currents by using whole-cell patch clamp technique.Immunocytochemistry was used to examine the expression of the proteins in ICCs.RESULTS:LPS suppressed the pacemaker currents of ICCs and this could be blocked by AH6809,a prostaglandin E2-EP2 receptor antagonist or NG-Nitro-Larginine Methyl Ester,an inhibitor of nitric oxide(NO) synthase.Toll-like receptor 4,inducible NO synthase or cyclooxygenase-2 immunoreactivity by specific antibodies was detected on ICCs.Catalase(antioxidant agent) had no action on LPS-induced action in ICCs.LPS actions were blocked by nuclear factor kB(NF-kB) inhibitor,actinomycin D(a gene transcription inhibitor),PD 98059(a p42/44 mitogen-activated protein kinases inhibitor) or SB 203580 [a p38 mitogen-activated protein kinases(MAPK) inhibitor].SB 203580 also blocked the prostaglandin E2-induced action on pacemaker currents in ICCs but not NO.CONCLUSION:LPS inhibit the pacemaker currents in ICCs via prostaglandin E2-and NO-dependent mechanism through toll-like receptor 4 and suggest that MAPK and NF-kB are implicated in these actions.

关键词： Interstitial cells of Cajal Lipopolysaccharide Mitogen-activated protein kinases Nuclear factor kB Small intestine

WheatNet： a Genome-Scale Functional Network for Hexaploid Bread Wheat, Triticum aestivum

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Molecular Plant 2017年第8期10卷 1133-1136页

作者： Tak Lee sohyun Hwang Chan Yeong Kim Hongseok Shim Hyojin Kim Pamela C. Ronald EdwardM. Marcotte insuk Lee Department of Biotechnology College of Life Science and Biotechnology Yonsei University Seou1120-749 Korea Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology University of Texas at Austin Austin TX 78712 USA Department of Biomedical Science College of Life Science CHA University Seongnam-si 13496 Korea Department of Plant Pathology University of California Davis CA 95616 USA Joint Bioenergy Institute Emeryville CA 94608 USA The Genome Center University of California Davis CA 95616 USA Department of Molecular Bioseiences University of Texas at Austin Austin TX 78712 USA

Dear Editor Gene networks provide a system-level overview of genetic organizations and enable the dissection of functional modules underlying complex traits. Integration of diverse genomics data based on the Bayesian statistics framework has been successfully applied to the construction of genome-scale functional networks for major crop species such as rice （Lee et al., 2011）, soybean （Kim et al., 2017）, and tomato （Kim et al.,

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TomatoNet A Genome-wide Co-functional Network for Unveiling Complex Traits of Tomato, a Model Crop for Fleshy Fruits

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Molecular Plant 2017年第4期10卷 652-655页

作者： Hyojin Kim Bong Suk Kim Jung Eun Shim sohyun Hwang Sunmo Yang Eiru Kim Aniafi S. Iver-Pascuzzi2 insuk Lee Department of Biotechnology College of Life Science and Biotechnology Yonsei University Seou1120-749 Korea Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907 USA

Tomato （solanum lycopersicum） is the second most important vegetable crop globally, with a total world production valued at over $74 billion （FAOSTAT Statistics Database, 2009）. Tomato is also an important model c... 详细信息

关键词： Genome-wide Co-functional Network Unveiling Complex Traits Tomato

LSA Based Classification of Advertising Spam Reviews

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Computer Technology and Application 2011年第12期2卷 998-1006页

作者： insuk Park Hanhoon Kang Seong Joon Yoo Department of Computer Engineering Sejong University 98 Gunja Gwangfin Seoul 143-747 Korea