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Temporal-spatial Generation of Astrocytes in the Developing Diencephalon

Neuroscience Bulletin 2024年第1期40卷 1-16页

作者： Wentong Hong Pifang gong Xinjie Pan Zhonggan Ren Yitong Liu Guibo qi Jun-Liszt Li Wenzhi Sun Woo-Ping Ge Chun-Li Zhang Shumin Duan Song qin Department of Anatomy Histology and EmbryologySchool of Basic Medical SciencesFudan UniversityShanghai 200032China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China Chinese Institute for Brain Research Beijing 102206China School of Basic Medical Sciences Capital Medical UniversityBeijing 100069China Department of Molecular Biology University of Texas Southwestern Medical CenterDallasTexas 75390-9148USA Department of Neurobiology Key Laboratory of Medical Neurobiology of Ministry of Health of ChinaZhejiang University School of MedicineHangzhou 310058China State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghai 200032China

tAstrocytes are the largest glial population in the mammalian ***,we have a minimal understanding of astrocyte development,especially fate specification in different regions of the *** lineage tracing of the progenitors of the third ventricle(3V)wall via in-utero electroporation in the embryonic mouse brain,we show the fate specification and migration pattern of astrocytes derived from radial glia along the 3V ***,radial glia located in different regions along the 3V wall of the diencephalon produce distinct cell types:radial glia in the upper region produce astrocytes and those in the lower region produce neurons in the *** genetic fate mapping analysis,we reveal that the first population of astrocytes appears along the zona incerta in the *** occurs at an early time point in the dorsal region relative to that in the ventral region of the developing *** transcriptomic analysis of the region-specific 3V wall and lateral ventricle(LV)wall,we identified cohorts of differentially-expressed genes in the dorsal 3V wall compared to the ventral 3V wall and LV wall that may regulate astrogenesis in the dorsal ***,these results demonstrate that the generation of astrocytes shows a spatiotemporal pattern in the developing mouse diencephalon.

关键词： Radial glia Astrocyte specification Lineage tracing Diencephalon Third ventricle

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用于胸片分类的自校正特征融合金字塔网络

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天津大学学报（自然科学与工程技术版） 2024年第5期57卷 511-520页

作者：宫霄霖程琦李锵天津大学微电子学院天津300072

胸部疾病的病灶区域面积差异较大,易受健康部位的影响,难以定位,并且用于诊断疾病的X射线胸片样本数据分布不平衡,导致胸部疾病分类网络的分类准确度较低.针对胸部疾病分类任务中仍然存在的问题和挑战,本文提出了一种自校正特征融合金... 详细信息

胸部疾病的病灶区域面积差异较大,易受健康部位的影响,难以定位,并且用于诊断疾病的X射线胸片样本数据分布不平衡,导致胸部疾病分类网络的分类准确度较低.针对胸部疾病分类任务中仍然存在的问题和挑战,本文提出了一种自校正特征融合金字塔网络.该网络使用自校正卷积增强疾病部位在特征图上以及通道之间的位置联系,在不引入额外参数量的条件下增大了卷积的感受野,避免无病区域的信息干扰;然后通过特征融合金字塔网络整合多尺度图像特征信息,在获取高分辨率特征图的同时,能够准确地定位病变区域,可以更好地识别不同尺度疾病的特征,在多标签分类任务上具有独特的优势;同时引入频率通道注意力机制强化网络对疾病特征的提取能力,在上采样和特征融合变换之前,减少全局平均池化过程中造成的特征丢失;最后提出一种轮次焦点损失函数区分不同种类胸部疾病的训练程度,根据分类难易程度区分样本,并在不同的训练轮次调整权重,以解决胸部疾病样本分布不平衡问题.在ChestX-ray14数据集上的平均AUC值可达0.853,在CheXpert数据集上的平均AUC值可达0.903,超过了近年来较为先进的网络模型.实验结果表明,该网络与传统的胸部疾病分类网络相比能有效地提高胸部疾病的分类精度,并且具有较强的泛化能力.

关键词：胸部疾病自校正卷积特征融合金字塔网络频率通道注意力轮次焦点损失函数

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Growth hormone promotes the reconstruction of injured axons in the hypothalamo-neurohypophyseal system

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Neural Regeneration Research 2024年第10期19卷 2249-2258页

作者： Kai Li Zhanpeng Feng Zhiwei Xiong Jun Pan Mingfeng Zhou Weizhao Li Yichao Ou Guangsen Wu Mengjie Che Haodong gong Junjie Peng Xingqin Wang Songtao qi Junxiang Peng Department of Neurosurgery Institute of Brain DiseasesNanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Previous studies have shown that growth hormone can regulate hypothalamic energy metabolism, stress, and hormone release. Therefore, growth hormone has great potential for treating hypothalamic injury. In this study, we established a specific hypothalamic axon injury model by inducing hypothalamic pituitary stalk electric lesions in male mice. We then treated mice by intraperitoneal administration of growth hormone. Our results showed that growth hormone increased the expression of insulin-like growth factor 1 and its receptors, and promoted the survival of hypothalamic neurons, axonal regeneration, and vascular reconstruction from the median eminence through the posterior pituitary. Altogether, this alleviated hypothalamic injury-caused central diabetes insipidus and anxiety. These results suggest that growth hormone can promote axonal reconstruction after hypothalamic injury by regulating the growth hormone-insulin-like growth factor 1 axis.

关键词： arginine vasopressin growth hormone hypothalamo-neurohypophyseal system hypothalamus injury insulin-like growth factor 1 oxytocin regeneration

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Clinicopathological significance and immunotherapeutic outcome of claudin 18.2 expression in advanced gastric cancer:A retrospective study

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Chinese Journal of Cancer Research 2024年第1期36卷 78-89页

作者： Changsong qi Xiaoyi Chong Ting Zhou Mingyang Ma Jifang gong Miao Zhang Jian Li Jun Xiao Xiaohui Peng Zhen Liu Zonghai Li Lin Shen Xiaotian Zhang State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDepartment of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China CARsgen Therapeutics Co. LtdShanghai 200231China CARsgen Life Sciences Co. LtdShanghai 200131China

Objective: Immunotherapeutic outcomes and clinical characteristics of claudin 18 isoform 2 positive(CLDN18.2-positive) gastric cancer(GC) vary in different clinical studies, making it difficult to optimize antiCLDN18.2 therapy. We conducted a retrospective analysis to explore the association of CLDN18.2 expression with clinicopathological characteristics and immunotherapeutic outcomes in ***: A total of 536 advanced GC patients from 2019 to 2021 in the CT041-CG4006 and CT041-ST-01clinical trials were included in the analysis. CLDN18.2 expression on ≥40% of tumor cells(2+, 40%) and CLDN18.2 expression on ≥70% of tumor cells(2+, 70%) were considered the two levels of positively expressed GC. The clinicopathological characteristics and immunotherapy outcomes of GC patients were analyzed according to CLDN18.2 expression ***: CLDN18.2 was expressed in 57.6%(cut-off: 2+, 40%) and 48.9%(cut-off: 2+, 70%) of *** death-ligand 1(PD-L1) and CLDN18.2 were co-expressed in 19.8% [combined positive score(CPS)≥1, CLDN18.2(cut-off: 2+, 40%)] and 17.2% [CPS≥5, CLDN18.2(cut-off: 2+, 70%)] of ***18.2 expression positively correlated with younger age, female sex, non-gastroesophageal junction(nonGEJ), and diffuse phenotype(P<0.001). HER2 and PD-L1 expression were significantly lower in CLDN18.2-positive GC(both P<0.05). Uterine adnexa metastasis(P<0.001) was more frequent and liver metastasis(P<0.001)was less common in CLDN18.2-positive GC. Overall survival and immunotherapy-related progression-free survival(ir PFS) were inferior in the CLDN18.2-positive ***: CLDN18.2-positive GC is associated with poor prognosis and worse immunotherapeutic outcomes. The combination of anti-CLDN18.2 therapy, anti-PD-L1/PD-1 therapy, and chemotherapy for GC requires further investigation.

关键词： Gastric cancer claudin18.2 CT041 PD-L1 immunotherapy

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高压坩埚-草酸-盐酸羟胺法提取粘土中的游离氧化铁

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分析化学 1999年第6期27卷 665-667页

作者：龚琦广西大学化学化工学院南宁530004

为更准确地测定粘土中的游离氧化铁,提出一个提取游离氧化铁的新方法.该方法是:在高压坩埚中用草酸和盐酸羟胺混合液作为提取剂,于130℃的密封条件下提取粘土中的游离氧化铁,浸提时间为0.5h.实验表明:本法较常用的连二亚硫酸钠-柠檬酸钠... 详细信息

为更准确地测定粘土中的游离氧化铁,提出一个提取游离氧化铁的新方法.该方法是:在高压坩埚中用草酸和盐酸羟胺混合液作为提取剂,于130℃的密封条件下提取粘土中的游离氧化铁,浸提时间为0.5h.实验表明:本法较常用的连二亚硫酸钠-柠檬酸钠-重碳酸钠法能更有效地分解粘土中的铁的氧化物,使游离氧化铁的浸出更完全.方法的RSD小于2.5%.

关键词：游离氧化铁提取粘土高压坩涡草酸盐酸羟胺

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The expression mechanism of programmed cell death 1 ligand 1 and its role in immunomodulatory ability of mesenchymal stem cells

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Chinese Journal of Traumatology 2024年第1期27卷 1-10页

作者： Zhuo Chen Meng-Wei Yao Xiang Ao qing-Jia gong Yi Yang Jin-Xia Liu qi-Zhou Lian Xiang Xu Ling-Jing Zuo Department of Stem Cell&Regenerative Medicine State Key Laboratory of TraumaBurn and Combined InjuryDaping HospitalArmy Medical UniversityChongqing400042China College of Basic Medical Sciences Army Medical UniversityChongqing400038China Department of Orthopedics 953 Hospital of PLAShigatse Branch of Xinqiao HospitalArmy Medical UniversityShigatse857000Tibet Autonomous RegionChina Department of Rheumatology and Immunology Daping HospitalArmy Medical UniversityChongqing400042China Department of Obstetrics and Gynecology Chongqing People's HospitalChongqing401121China Department of Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina Department of Nuclear Medicine The First People's Hospital of Yunnan provinceAffiliated Hospital of Kunming University of Science and TechnologyKunming650034China

Programmed cell death 1 ligand 1(PD-L1)is an important immunosuppressive molecule,which inhibits the function of T cells and other immune cells by binding to the receptor programmed cell *** PD-L1 expression disorder plays an important role in the occurrence,development,and treatment of sepsis or other inflammatory diseases,and has become an important target for the treatment of these *** stem cells(MSCs)are a kind of pluripotent stem cells with multiple differentiation *** recent years,MSCs have been found to have a strong immunosuppressive ability and are used to treat various inflammatory insults caused by hyperimmune ***,PD-L1 is deeply involved in the immunosuppressive events of MSCs and plays an important role in the treatment of various *** this review,we will summarize the main regulatory mechanism of PD-L1 expression,and discuss various biological functions of PD-L1 in the immune regulation of MSCs.

关键词： PD-L1 MSCs Immunomodulation

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Cultivating the uncultured:Harnessing the“sandwich agar plate”approach to isolate heme-dependent bacteria from marine sediment

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mLife 2024年第1期3卷 143-155页

作者： Jing Zhang qi-Yun Liang Da-Shuai Mu Fengbai Lian Ya gong Mengqi Ye Guan-Jun Chen Yuqi Ye Zong-Jun Du State Key Laboratory of Microbial Techlogy Insttute of Micrbial TchnoogyShandong UniversityQingdaoChina Marine College Shandong UniverstyWeihaiChina Shandong University-Weihai Research Institute of Industrial Technology WeihaiChina

In the classical microbial isolation technique,the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for *** this study,we designed a simple coculture technique named the“sandwich agar plate method,”which maintains microbial interactions throughout the isolation and pure culture *** total yield of uncultured species in sandwich agar plates based on eight helper strains was almost 10-fold that of the control *** uncultured species displayed commensal *** study found that heme was the growth-promoting factor of some marine commensal *** genomic analysis revealed that heme auxotrophies were common in various biotopes and prevalent in many uncultured microbial ***,our study supported that the survival strategies of heme auxotrophy in different habitats varied *** findings highlight that cocultivation based on the“sandwich agar plate method”could be developed and used to isolate more uncultured bacteria.

关键词： cultivation growth factors heme sandwich agar plate uncultured bacteria

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A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences

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Journal of Genetics and Genomics 2019年第7期46卷 363-366页

作者： Jiantao Tan qi gong Suize Yu Yuke Hou Dongchang Zeng qinlong Zhu Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China

Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology *** this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al.

关键词： Ballester PCR Whittier

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基于反双曲正切函数的变步长LMS算法

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北京理工大学学报 2022年第10期42卷 1051-1058页

作者：火元莲安娅琦巩琪连培君西北师范大学物理与电子工程学院甘肃兰州730070

针对定步长的LMS算法无法同时满足低稳态误差和快收敛速度这个需求,本文提出了一种基于反双曲正切函数的变步长LMS算法.该算法基于反双曲正切函数构建步长与误差信号之间的非线性函数关系式,以此来替代LMS算法中的定步长,实现了对步长... 详细信息

针对定步长的LMS算法无法同时满足低稳态误差和快收敛速度这个需求,本文提出了一种基于反双曲正切函数的变步长LMS算法.该算法基于反双曲正切函数构建步长与误差信号之间的非线性函数关系式,以此来替代LMS算法中的定步长,实现了对步长因子的动态调整.文中详细讨论了新的变步长函数中参数α,β和γ对于算法性能的影响,并和其他几种较新的变步长算法进行了性能比较.仿真结果表明,所提算法很好地兼顾了收敛速度、稳态误差和跟踪性能,在系统辨识、正弦信号去噪和自适应线性预测方面表现出了优异的性能.

关键词：自适应滤波最小均方算法变步长LMS算法反双曲正切函数

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Icariin improves APP/PS1 mice's cognitive function through reducing endoplasmic reticulum stress and apoptosis

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中国药理学与毒理学杂志 2018年第9期32卷 696-697页

作者： ZHANG Yang-yang LU Xiao-feng gong qi-hai LI Fei Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical University Zunyi 563006China The People's Hospital of Honghuagang District Zunyi 56

OBJECTIVE Alzheimer disease(AD) is a progressive neurodegenerative disorder involving a gradual decline in many cognitive processes and in *** endoplasmic reticulum(ER) is involved in several crucial cellular functions,eg protein folding and quality *** misfolded or unfolded proteins in ER can disturb the function of ER and induce ER stress,which results in neuronal death in ***(ICA) has a wide range of neuro protection and has been researched in AD ***,whether ICA has the effect on ER stress in AD condition,and how ICA affects ER stress remains stil ***,the current study aimed to investigate the mechanism of ICA against cognitive impairments in AD model through ER stress pathway and *** Twelve months male APP/PS1 or wild-type(WT)mice were randomly divided into four groups:APP/PS1,and APP/PS1+ICA,WT and WT+ICA *** treated mice were given ICA60 mg·kg-1 per day and control mice were received the same volume distilled water for consecutive 3 *** Morris water maze and novel object recognition were used to detect animals′ *** staining was used to observe the neuronal morphology in hippocampus *** protein and(or) phosphorylation level of GRP78,p-PERK,PERK,p-IRE1,IRE1,ATF6,p-e IF2α,eIF2α,ATF4,CHOP,the level of cleaved-casepase 3,Bax and Bcl-2 were examined by Western *** The behavior performance testing by Morris water maze and novel object recognition deteriorated in APP/PS1 mice compared with WT mice,however,ICA significantly improved the behavior performance when compared with APP/*** neuron impairments in APP/PS1 mice also were ameliorated after ICA *** protein expression of GRP78,ATF4,CHOP,and the level of p-PERK and p-eI F2α were higher in APP/PS1 mice than that in WT *** Bax/Bcl-2 ratio elevation and caspase 3 activation have been found in APP/PS1 *** treated with ICA,those above-mentioned parameters were decreased compared with A

关键词： icariin endoplasmic reticulum stress apoptosis behavior Alzheimer disease

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