Cultivating the uncultured:Harnessing the“sandwich agar plate”approach to isolate heme-dependent bacteria from marine sediment
作者机构：State Key Laboratory of Microbial TechlogyInsttute of Micrbial TchnoogyShandong UniversityQingdaoChina Marine CollegeShandong UniverstyWeihaiChina Shandong University-Weihai Research Institute of Industrial TechnologyWeihaiChina
出 版 物：《mLife》 (微生物（英文）)
年 卷 期：2024年第3卷第1期
页 面：143-155页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(Nos.32070002 and 41876166) Science&Technology Fundamental Resources Investigation Program(Grant Nos.2022FY101100 and 2019FY100700) China Postdoctoral Science Foundation(2022M721923) Natural Science Foundation of Shandong Province,China(ZR2023QD187)
主 题：cultivation growth factors heme sandwich agar plate uncultured bacteria
摘 要：In the classical microbial isolation technique,the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for *** this study,we designed a simple coculture technique named the“sandwich agar plate method,which maintains microbial interactions throughout the isolation and pure culture *** total yield of uncultured species in sandwich agar plates based on eight helper strains was almost 10-fold that of the control *** uncultured species displayed commensal *** study found that heme was the growth-promoting factor of some marine commensal *** genomic analysis revealed that heme auxotrophies were common in various biotopes and prevalent in many uncultured microbial ***,our study supported that the survival strategies of heme auxotrophy in different habitats varied *** findings highlight that cocultivation based on the“sandwich agar plate methodcould be developed and used to isolate more uncultured bacteria.
维普期刊数据库