A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences

作     者：Jiantao Tan Qi Gong Suize Yu Yuke Hou Dongchang Zeng Qinlong Zhu Yao-Guang Liu Jiantao Tan;Qi Gong;Suize Yu;Yuke Hou;Dongchang Zeng;Qinlong Zhu;Yao-Guang Liu

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2019年第46卷第7期

页      面：363-366页

核心收录：

学科分类：0710[理学-生物学] 10[医学] 

基　　金：supported by grants from the Ministry of Agriculture of China (2016ZX08010001) the National Natural Science Foundation of China (31771740) 

主　　题：Ballester PCR Whittier 

摘      要：Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology *** this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al.