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BIOCELL 2020年第3期44卷 421-429页

作者： HAO WANG fang fang chaoyi fang RUNSHENG ZHAO SHAOXIAN WANG School of Preclinical Medicine Hebei University of Chinese MedicineShijiazhuangChina Department of Oncology Hebei Provincial Hospital of Traditional Chinese MedicineShijiazhuangChina

Stress is an inevitable interference factor that seriously affects *** to music is an economical,noninvasive,and highly accepted tool for easing ***,physiological studies investigating the ability of music to reduce stress in daily life are *** established rat models of chronic immobilization stress(CIS)to observe changes in the hypothalamic arcuate nucleus(ARC)neurons involved in the regulation of food intake and the effect of comfortable classical music ***-one days of stress resulted in decreased food intake and delayed body weight gain,up-regulation of leptin receptor(Ob-R),cocaine-and amphetamine-regulated transcript(CART),proopiomelanocortin(POMC),and alpha-melanocyte-stimulating hormone(α-MSH)expression,and downregulation of neuropeptide Y(NPY)and agouti-related protein(AgRP)expression in the ***,peripheral leptin entered the ARC under chronic stress conditions,bound to Ob-R,and affected downstream nerve pathways related to appetite,such as the NPY/AgRP and CART/POMC *** classical music played at 65 dB reversed the abnormal expression of Ob-R and NPY induced by chronic ***,listening to comfortable music improves changes in ARC neurons related to the regulation of food intake in CIS rats,and these results provide a reference for basic research regarding how music therapy alleviates stress and stress-related health issues.

关键词： Hypothalamic arcuate nucleus Neuropeptides Food intake Body weight Chronic immobilization stress Music exposure

Danggui Buxue Tang ameliorates bleomycin-induced pulmonary fibrosis in rats through inhibiting transforming growth factor-β1/Smad3/plasminogen activator inhibitor-1 signaling pathway

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Journal of Traditional Chinese Medicine 2020年第2期40卷 236-244页

作者： Wang Jiepeng fang chaoyi Wang Shaoxian fang fang Chu Xinqiao Liu Na Lu Chenxi Wang Shuo Li Wei School of Preclinical Medicine Hebei University of Chinese MedicineShijiazhuang 050200China Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease Hebei University of Chinese MedicineShijiazhuang 050091China

OBJECTIVE: To investigate the effect of Danggui Buxue Tang(DBT), a decoction from Traditional Chinese Medicine, on bleomycin-induced pulmonary fibrosis in rats, and to propose the possible underlying ***: Forty male Sprague-Dawley rats were randomly divided into sham group, model group,prednisone group and DBT group. Pulmonary fibrosis rat model was established by intratracheal injection with bleomycin. Body weight and lung index were monitored. Histopathologic examination and collagen deposition were determined using Hematoxylin and eosin(HE) and Masson's trichrome staining. Immunohistochemistry staining was applied to observe the expression of alpha-smooth muscle actin(α-SMA). m RNA expression of α-SMA,collagen Ⅰ and collagen Ⅲ were measured by realtime fluorescence quantitative PCR(RT-q PCR). Inflammatory cytokines, including tumor necrosis factor alpha(TNF-α), interleukin-6(IL-6) and IL-1β in serum were detected by Enzyme-linked immunosorbent assay. Alkali hydrolysis method was conducted to investigate the content of hydroxyproline(HYP). Transforming growth factor-β1(TGF-β1),Smad3 and plasminogen activator inhibitor-1(PAI-1) protein level were examined by Western blot ***: DBT significantly reduced the severity of bleomycin-induced pulmonary fibrosis and inflammation as indicated by minimizing the lost of weight, and by lowering the levels of lung index, inflammation score, Ashcroft score, collagen volume fraction(%), HYP, α-SMA, collagen Ⅰ, collagen Ⅲ,TNF-α, IL-6, IL-1β, TGF-β1, Smad3 and PAI-1, consistent with the effect of ***: Our findings suggest that DBT is able to ameliorate the pulmonary fibrosis, the possible mechanism may involve inhibition of pulmonary inflammation and collagen deposition, possibly via suppressing TGF-β1/Smad3/PAI-1 signaling pathway.

关键词： Pulmonary fibrosis Transforming growth factor beta 1 Smad3 protein Plasminogen inactivators Danggui Buxue Tang

Xiaoyaosan decoction regulates food intake and body weight via the leptin-leptin receptor-proopiomelanocortin pathway in rats with chronic immobilization stress

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Journal of Traditional Chinese Medicine 2019年第1期39卷 34-44页

作者： Wang Xia fang chaoyi Wu Jiaxin Wang Shaoxian Zhao Runsheng Ma Jinsheng School of Preclinical Medicine Hebei University of Chinese MedicineShijiazhuang 050200China.

OBJECTIVE: To evaluate the effects of the Xiaoyaosan(XYS) decoction on the food intake and body weight of rats with chronic immobilization stress(CIS), as well as the concentration of serum leptin and the expression of feeding-related neuropeptides [leptin receptor(Ob-R), proopiomelanocortin(POMC), and α-melanocyte-stimulating hormone(α-MSH)] in the hypothalamic arcuate nucleus(ARC).METHODS: We subjected rats to CIS for 3 h a *** rats were randomly divided into the following groups: control group, 7-day stress group, 21-day stress group and XYS-treated group. The rats in the two stress groups were exposed to CIS for 7 or 21 *** rats in the XYS-treated group were also exposed to CIS for 21 d and were intragastrically administered the XYS decoction before stress. The body weight and food intake of the rats were measured every day. The content of leptin in serum andα-MSH in the ARC were detected by ELISA, and the expression of neuropeptides in the ARC was assayed by immunofluorescence, Western blot and q ***: The food intake and body weight of rats exposed to CIS were lower than those of control rats. The serum leptin, and expression levels of ObR, POMC and α-MSH in the ARC were significantly higher than those in the control rats. Treatment with the XYS decoction improved the appetite and the body weight, and down-regulated serum leptin and Ob-R, POMC and α-MSH in hypothalamus ***: The leptin-Ob-R-POMC pathway might be the part of the mechanism underlying XYS's improvement of somatic symptoms such as reduction in food intake and body weight related to CIS.

关键词： Chronic immobilization stress Xiao Yao San Neuropeptides Arcuate nucleus of hypothalamus Eating

Bushenhuoxue improves cognitive function and activates brain-derived neurotrophic factor-mediated signaling in a rat model of vascular dementia

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Journal of Traditional Chinese Medicine 2020年第1期40卷 49-58页

作者： Wang Wei Zhang Yixin Yu Wentao Gao Weijuan Shen Ning Jin Bing Wang Xiangting fang chaoyi Wang Yanjun General Medical Practice Shuangyushu Community Health Service Center of Haidian DistrictBeijing 100086China Hebei University of Chinese Medicine ShijiazhuangHebei 050200China Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease Shijiazhuang 050091China Acupuncture and Moxibustion Hospital of Chinese Academy of Chinese Medical Sciences Beijing 100700China Department of Geriatrics Xiyuan Hospital of Chinese Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the protective mechanisms of the Traditional Chinese Medicine Bushenhuoxue(BSHX)in a rat model of vascular dementia(VD).METHODS:A rat model of VD was developed using bilateral common carotid artery occlusion(BCCAO).Rats were administered BSHX(10.14 or 5.07 g/kg),nimodipine(11.06 mg/kg;positive control),or saline(control)by gavage daily for 30 d *** and memory abilities were assessed using the Morris water *** changes in the hippocampus were observed using light microscopy(hematoxylin and eosin staining)and transmission electron microscopy(TEM).The m RNA and protein expression levels of brain-derived neurotrophic factor(BDNF),tyrosine receptor kinase B(Trk B),phosphatidyl inositol 3-kinase(PI3 K),serine/threonine kinase(AKT),and c AMP response element binding protein(CREB)were measured by real-time polymerase chain reaction(RT-PCR)and Western blot,***:Compared with the sham group,rats with BCCAO exhibited impaired learning and memory abilities(Morris water maze)and showed abnormalities in neuronal morphology(light microscopy)and ultrastructure(TEM)in the *** also had decreased m RNA and protein expressions of BDNF,Trk B,PI3 K,AKT,and CREB in hippocampal tissue(all P<0.05).In rats with BCCAO,administration of BSHX attenuated deficits in learning and memory,improved the morphology and ultrastructure of hippocampal neurons,and enhanced m RNA and protein expression levels of BDNF,Trk B,PI3 K,AKT,and CREB(all P<0.05).CONCLUSION:BSHX may protect hippocampal neurons and improve learning and memory abilities,at least in part via the activation of BDNF/Trk B/PI3 K/AKT/CREB signaling.

关键词： Dementia,vascular Brain-derived neurotrophic factor Phosphatidylinositol 3-kinase Protein serine-threonine kinases Bushenhuoxue prescription

Qinghua decoction(清化饮)improves chronic nonbacterial prostatitis possibly via regulating the chromogranin A/nerve growth factor/tyrosine kinase A signaling pathway mediated by inflammatory factors

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Journal of Traditional Chinese Medicine 2023年第4期43卷 695-703页

作者： HAN Yunpeng YU Wentao ZHANG Ying XU Huazhou DENG Guoxing fang chaoyi School of Basic Medical Sciences Hebei University of Chinese MedicineShijiazhuang 050200China Hebei Key Laboratory of Integrated Chinese and Western Medicine for Lung Disease Research Shijiazhuang 050091China

OBJECTIVE:To explore the mechanism by which Qinghua decoction(清化饮)regulates neuroendocrine inflammation in chronic nonbacterial prostatitis(CNP)model rats and provide an experimental basis for clinical ***:The rats were randomly divided into six groups:normal control,model,Qianlie Tongyu capsule,low-dose Qinghua decoction,medium-dose Qinghua decoction,and high-dose Qinghua decoction group with six rats in each *** in each group were sacrificed on the 29th day of treatment,and blood and prostate tissues were *** levels of tumor necrosis factor-alpha and interleukins 1-beta,6,8,and 10(TNF-αand IL-1β,-6,-8,and-10,respectively)were measured using enzyme-linked immunosorbent *** pathological changes in the rat prostate tissue in each group were observed under a light *** expression levels of chromogranin A(CgA),nerve growth factor(NGF),and tyrosine kinase A(TrkA)were detected using reverse transcription quantitative polymerase chain *** blotting was used to detect protein expression of CgA,NGF,and ***:In the model group,the prostate capsule membrane and stroma were significantly dilated with more inflammatory cells infiltrating the stroma and ***-α,IL-1β,-6,and-8,CgA,NGF,and TrkA levels increased,whereas the content of IL-10 decreased,which was statistically significant compared to that in the normal control group(P<0.05).Prostate tissue cells in the high-dose group were neatly arranged with no obvious inflammatory cell *** compared with the model group,the high-dose Qinghua decoction group showed a significant improvement in these indices(P<0.05).CONCLUSION:Qinghua decoction led to inhibition of pathological changes in the prostate tissue of rats with CNP,regulation of inflammatory cytokine expression,and inhibition in the expression of CgA,NGF,and *** mechanism may be primarily related to regulation of the CgA/NGF/TrkA signaling pathway mediated by various inflammato

关键词： inflammation interleukins chromogranin A nerve growth factor chronic nonbacterial prostatitis Qinghua decoction

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国际黄金价格走势分析与预测方法

黄金 2009年第11期30卷 4-7页

作者：方超逸华东师范大学金融学系

布雷顿森林体系崩溃后,黄金从官方限价转变为市场定价,金价走势也一直起伏不定。由于黄金的特殊属性和历史地位,以及政治、经济等各方面因素都会影响金价,所以金价走势一直难以预测。文中从分析黄金合理价格标准入手,探求判断金价走势... 详细信息

布雷顿森林体系崩溃后,黄金从官方限价转变为市场定价,金价走势也一直起伏不定。由于黄金的特殊属性和历史地位,以及政治、经济等各方面因素都会影响金价,所以金价走势一直难以预测。文中从分析黄金合理价格标准入手,探求判断金价走势的新方法,并且得出结论:通过黄金期货持仓量增减把握短期金价走势;根据美元指数和石油价格评估中期金价走势;按照美元购买力大小推算长期金价走势。

关键词：合理金价标准金价走势判断实际金价

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VR/AR技术在服装行业中的发展与应用

现代纺织技术 2022年第6期30卷 166-175页

作者：方超逸何佳臻苏州大学纺织与服装工程学院江苏苏州215006

为进一步推动服装行业数字化发展以及拓宽虚拟现实(VR)和增强现实(AR)技术在服装行业的应用,介绍了VR与AR涉及到的具体技术及其发展轨迹,并总结了VR/AR技术目前的主要应用领域。围绕整个服装产业链,在设计环节利用VR/AR技术能够开展面... 详细信息

为进一步推动服装行业数字化发展以及拓宽虚拟现实(VR)和增强现实(AR)技术在服装行业的应用,介绍了VR与AR涉及到的具体技术及其发展轨迹,并总结了VR/AR技术目前的主要应用领域。围绕整个服装产业链,在设计环节利用VR/AR技术能够开展面料设计、服装款式及结构设计;在性能评价环节利用该技术模拟服装使用的真实场景,实现基于虚拟场景的着装舒适性评价;在营销策划环节,利用该技术增强T台秀及虚拟试衣的视觉体验。最后,从硬件和软件等方面对VR/AR技术进行了总结与展望,指出算法升级及应用生态构建是未来的发展方向。

关键词： VR AR 服装数字化服装生产环节服装智能化

基于AGEs/RAGE/NF-κB通路探讨补肝散改善D-半乳糖致衰大鼠学习记忆障碍机制研究

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Digital Chinese Medicine 2023年第3期6卷 317-327页

作者：李伟王硕王杰鹏方芳方朝义河北中医药大学基础医学院中国河北石家庄050200 河北省中西医结合肺病研究重点实验室中国河北石家庄050091

目的探讨复方补肝散(BGSD)干预D-半乳糖致衰大鼠学习记忆能力的潜在作用机制。方法40只大鼠被随机分为对照组、模型组、BGSD[14.06 g/(kg·d)]组和吡拉西坦[0.4 g/(kg·d)]组,每组10只。腹腔注射D-半乳糖[400 mg/(kg·d)]建立衰老大鼠... 详细信息

目的探讨复方补肝散(BGSD)干预D-半乳糖致衰大鼠学习记忆能力的潜在作用机制。方法40只大鼠被随机分为对照组、模型组、BGSD[14.06 g/(kg·d)]组和吡拉西坦[0.4 g/(kg·d)]组,每组10只。腹腔注射D-半乳糖[400 mg/(kg·d)]建立衰老大鼠模型。每周记录大鼠体质量、摄水量、摄食量和抓力;八臂迷宫和跳台实验评估大鼠学习记忆能力;称取肝脏、胸腺、脾脏和脑组织重量以计算相应脏器指数;检测血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;苏木精-伊红(HE)染色观察海马病理学改变;酶联免疫吸附法(ELISA)检测海马肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6及IL-1β水平;实时荧光定量PCR(RT-qPCR)检测海马晚期糖基化终末产物受体(RAGE)、核因子-κB(NF-κB)、TNF-α、IL-6和IL-1βmRNA表达;蛋白免疫印迹法(WB)检测海马晚期糖基化终末产物(AGEs)、RAGE和NF-κB蛋白表达。结果补肝散可显著增加D-半乳糖致衰大鼠的摄食量、摄水量、体质量、抓力及脏器指数(P<0.05),减少八臂迷宫中工作记忆错误(WME)、参考记忆错误(RME)以及总记忆错误次数(TE)(P<0.05),降低跳台实验中错误次数并延长跳台潜伏期(P<0.05)。此外,补肝散可减少海马神经元损伤,提高血清SOD活性,降低MDA含量及下调促炎因子TNF-α、IL-6和IL-1β水平(P<0.05)。进一步研究结果显示,补肝散可显著降低海马AGEs、RAGE和NF-κB表达(P<0.05)。结论补肝散可通过抑制AGEs/RAGE/NF-κB信号通路调节D-半乳糖致衰大鼠神经炎症损伤从而改善学习记忆能力。

关键词：补肝散肝气虚衰老学习记忆神经炎症 AGEs/RAGE/NF-κB信号通路

负载儿茶素的聚乙烯醇/壳聚糖电纺膜的构建及性能分析

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食品工业科技 2024年第4期45卷 10-23页

作者：方超逸肖菲菲陈宇桦邓仕彬莆田学院环境与生物工程学院福建莆田351100 福建省新型污染物生态毒理效应与控制重点实验室福建莆田351100 生态环境及其信息图谱福建省高等学校重点实验室福建莆田351100

为开发新型生物基食品包装材料,提高天然酚类化合物的生物利用度,本文以儿茶素(Catechin,CT)、聚乙烯醇(Polyvinyl alcohol,PVA)和壳聚糖(Chitosan,CS)为基材,通过静电纺丝法制备负载儿茶素的电纺膜。利用扫描电镜分析电纺膜的微观形貌... 详细信息

为开发新型生物基食品包装材料,提高天然酚类化合物的生物利用度,本文以儿茶素(Catechin,CT)、聚乙烯醇(Polyvinyl alcohol,PVA)和壳聚糖(Chitosan,CS)为基材,通过静电纺丝法制备负载儿茶素的电纺膜。利用扫描电镜分析电纺膜的微观形貌与直径分布;通过傅里叶红外光谱、X射线衍射、热分析等表征技术探讨CT与成膜基材之间的相互作用;以电纺膜的机械性能、气体透过率及抗氧化活性为参数,考察CT的添加量对电纺膜理化性能的影响;最后将电纺膜应用于草莓保鲜研究,通过感官评价并结合失水率、可滴定酸含量等指标分析电纺膜的保鲜效果。结果表明,CT与CS之间相互作用形成分子间氢键,疏水性能提高;当CT浓度为0.8%时,电纺膜的综合性能最佳:其形貌良好、结构致密,具有较好的热稳定性;此时其拉伸强度和断裂伸长率均达到最大值,分别为12.89 MPa和62.45%;其水溶性、水蒸气透过率和CO_(2)透过率均最低,分别为29.51%、0.1532 g·mm·(m^(2)·h·kPa)^(-1)和5.9 g·(m^(2)·h)^(-1);此时DPPH自由基清除率也达到最大值71.02%;并且电纺膜存在缓释作用,当CT浓度为0.8%时,其累积释放率最高;保鲜研究结果表明,电纺膜能有效延缓草莓的腐烂变质,并且0.8%CT浓度的电纺膜其保鲜效果最佳。综上,0.8%CT浓度的电纺膜,其综合性能最佳,具有一定的抗氧化性和保鲜效果。

关键词：静电纺丝聚乙烯醇壳聚糖儿茶素性能研究

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基于Rho/ROCK通路介导的细胞凋亡探讨补肝散对衰老大鼠心肌损伤的影响

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中药新药与临床药理 2022年第8期33卷 1032-1039页

作者：白德高张月霍冉王杰鹏方芳方朝义河北中医学院河北石家庄050200 河北省中西医结合肺病研究重点实验室河北石家庄050091

目的观察衰老大鼠心肌损伤特征并探讨补肝散的干预机制。方法将32只SPF级SD雄性大鼠随机分为空白组、模型组、补肝散组和维生素E组,除空白组腹腔注射等量生理盐水外,其余各组均腹腔注射D-半乳糖(D-galactose,D-gal)制备衰老大鼠模型。... 详细信息

目的观察衰老大鼠心肌损伤特征并探讨补肝散的干预机制。方法将32只SPF级SD雄性大鼠随机分为空白组、模型组、补肝散组和维生素E组,除空白组腹腔注射等量生理盐水外,其余各组均腹腔注射D-半乳糖(D-galactose,D-gal)制备衰老大鼠模型。模型复制当日起,补肝散组与维生素E组分别灌胃浓度为14.06 g·kg^(-1)的补肝散和27 mg·kg^(-1)的维生素E,空白组和模型组给予等量生理盐水。以上均每日1次,连续8周,并于模型复制末日测定抓力。取血清及心脏组织,测定心脏脏器指数;检测血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性变化;检测血清谷草转氨酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)含量;苏木素-伊红(HE)染色观察大鼠心肌组织病理形态学变化;Masson染色观察大鼠心肌组织胶原沉积和纤维化情况;TUNEL染色观察心肌细胞凋亡情况;免疫组织化学染色(ABC法)观察Ras同源基因A(RhoA)、Rho相关卷曲螺旋形成蛋白激酶1(ROCK1)、Bcl-2相关X蛋白(Bax)和B细胞淋巴瘤2(Bcl-2)表达特征;免疫印迹法(Western Blot)检测心肌组织RhoA、ROCK1、Bax和Bcl-2的蛋白表达。结果与空白组比较,模型组大鼠心肌细胞形态改变,呈现嗜酸性变性,胞质染色不均,部分心肌细胞坏死,心肌组织胶原沉积和纤维化明显;抓力及心脏脏器指数降低,MDA含量升高,SOD活性降低,心肌细胞凋亡指数及AST、CK、LDH含量升高(均P<0.05);RhoA、ROCK1、Bax蛋白表达增加,Bcl-2蛋白表达减少(均P<0.05)。与模型组比较,补肝散组大鼠心肌细胞轻度嗜酸性变性,偶有坏死、肥大情况,心肌组织胶原沉积较少,偶有纤维化产生,损伤情况较轻;大鼠抓力及心脏脏器指数升高,MDA含量降低,SOD活性升高,心肌细胞凋亡指数及AST、CK、LDH含量降低(均P<0.05);RhoA、ROCK1、Bax蛋白表达减少,Bcl-2蛋白表达增加(均P<0.05)。结论补肝散可通过调节Rho/ROCK介导的凋亡途径减轻衰老大鼠的心肌损伤,此为探讨肝气虚致衰理论提供了新的思路和实验支持。

关键词：衰老 Rho/ROCK信号通路细胞凋亡补肝散大鼠心肌损伤