Xiaoyaosan decoction regulates food intake and body weight via the leptin-leptin receptor-proopiomelanocortin pathway in rats with chronic immobilization stress

作     者：Wang Xia Fang Chaoyi Wu Jiaxin Wang Shaoxian Zhao Runsheng Ma Jinsheng 

作者机构：School of Preclinical MedicineHebei University of Chinese MedicineShijiazhuang 050200China. 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2019年第39卷第1期

页      面：34-44页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.81673881 and 81202644) the Natural Science Foundation of Hebei Province(No.H2016423049) 

主　　题：Chronic immobilization stress Xiao Yao San Neuropeptides Arcuate nucleus of hypothalamus Eating 

摘      要：OBJECTIVE: To evaluate the effects of the Xiaoyaosan(XYS) decoction on the food intake and body weight of rats with chronic immobilization stress(CIS), as well as the concentration of serum leptin and the expression of feeding-related neuropeptides [leptin receptor(Ob-R), proopiomelanocortin(POMC), and α-melanocyte-stimulating hormone(α-MSH)] in the hypothalamic arcuate nucleus(ARC).METHODS: We subjected rats to CIS for 3 h a *** rats were randomly divided into the following groups: control group, 7-day stress group, 21-day stress group and XYS-treated group. The rats in the two stress groups were exposed to CIS for 7 or 21 *** rats in the XYS-treated group were also exposed to CIS for 21 d and were intragastrically administered the XYS decoction before stress. The body weight and food intake of the rats were measured every day. The content of leptin in serum andα-MSH in the ARC were detected by ELISA, and the expression of neuropeptides in the ARC was assayed by immunofluorescence, Western blot and q ***: The food intake and body weight of rats exposed to CIS were lower than those of control rats. The serum leptin, and expression levels of ObR, POMC and α-MSH in the ARC were significantly higher than those in the control rats. Treatment with the XYS decoction improved the appetite and the body weight, and down-regulated serum leptin and Ob-R, POMC and α-MSH in hypothalamus ***: The leptin-Ob-R-POMC pathway might be the part of the mechanism underlying XYS s improvement of somatic symptoms such as reduction in food intake and body weight related to CIS.