Qinghua decoction(清化饮)improves chronic nonbacterial prostatitis possibly via regulating the chromogranin A/nerve growth factor/tyrosine kinase A signaling pathway mediated by inflammatory factors

作     者：HAN Yunpeng YU Wentao ZHANG Ying XU Huazhou DENG Guoxing FANG Chaoyi HAN Yunpeng;YU Wentao;ZHANG Ying;XU Huazhou;DENG Guoxing;FANG Chaoyi

作者机构：School of Basic Medical SciencesHebei University of Chinese MedicineShijiazhuang 050200China Hebei Key Laboratory of Integrated Chinese and Western Medicine for Lung Disease ResearchShijiazhuang 050091China 

出 版 物：《中医杂志:英文版》 (Journal of Traditional Chinese Medicine)

年 卷 期：2023年第43卷第4期

页      面：695-703页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Hebei Administration of Traditional Chinese Medicine Project:Study on the Neuroendocrine Mechanism of Chronic Prostatitis Rats Regulated by the Method of Clearing heat and Dampness,Activating Blood and Removing Stasis(No.2019087) Hebei Provincial Department of Education for Postgraduate Innovation Ability Training Project:the Mechanism of Improving Pulmonary Function of COPD Rats with Lung Qi Deficiency by Regulating Intestinal Bacteria with Peitu Shengjin Formula(No.CXZZBS2020151) Provincial Universities Basic Research Funds Special Project:Study on the Mechanism of Shenling Baizhu Powder Based on Lung Intestine Axis to Interfere with the Deficiency of Lung and Spleen in Experimental COPD(YJZ2019010) 

主　　题：inflammation interleukins chromogranin A nerve growth factor chronic nonbacterial prostatitis Qinghua decoction 

摘      要：OBJECTIVE:To explore the mechanism by which Qinghua decoction(清化饮)regulates neuroendocrine inflammation in chronic nonbacterial prostatitis(CNP)model rats and provide an experimental basis for clinical ***:The rats were randomly divided into six groups:normal control,model,Qianlie Tongyu capsule,low-dose Qinghua decoction,medium-dose Qinghua decoction,and high-dose Qinghua decoction group with six rats in each *** in each group were sacrificed on the 29th day of treatment,and blood and prostate tissues were *** levels of tumor necrosis factor-alpha and interleukins 1-beta,6,8,and 10(TNF-αand IL-1β,-6,-8,and-10,respectively)were measured using enzyme-linked immunosorbent *** pathological changes in the rat prostate tissue in each group were observed under a light *** expression levels of chromogranin A(CgA),nerve growth factor(NGF),and tyrosine kinase A(TrkA)were detected using reverse transcription quantitative polymerase chain *** blotting was used to detect protein expression of CgA,NGF,and ***:In the model group,the prostate capsule membrane and stroma were significantly dilated with more inflammatory cells infiltrating the stroma and ***-α,IL-1β,-6,and-8,CgA,NGF,and TrkA levels increased,whereas the content of IL-10 decreased,which was statistically significant compared to that in the normal control group(P0.05).Prostate tissue cells in the high-dose group were neatly arranged with no obvious inflammatory cell *** compared with the model group,the high-dose Qinghua decoction group showed a significant improvement in these indices(P0.05).CONCLUSION:Qinghua decoction led to inhibition of pathological changes in the prostate tissue of rats with CNP,regulation of inflammatory cytokine expression,and inhibition in the expression of CgA,NGF,and *** mechanism may be primarily related to regulation of the CgA/NGF/TrkA signaling pathway mediated by various inflammato