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In-situ formation of“electron conductive wires”threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

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Nano Research 2023年第2期16卷 2866-2874页

作者： Yu Lv Mingshi Deng Xuefeng Wang Xinghua Gao Lingyan Feng Wei Chen Chung-Chiun Liu Xinxin Li Yuan Zhang Materials Genome Institute Shanghai UniversityShanghai 200444China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Department of Emergency Tongji HospitalTongji University School of MedicineShanghai 200065China Department of Chemical and Biomolecular Engineering Case Western Reserve UniversityClevelandOH 44106

To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments,a rational engineering strategy for designing electrochemical immunosensing platform is ***,we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins(IL-6 and IL-8).For the construction of an efficient sensing interface,the conductive silver nanowires(Ag NWs)wrapped with zeolitic imidazolate framework(ZIF-8)thin film(denoted as ZIF-8@Ag NWs)are prepared,and then employed for the multi-functionalization of *** immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ions in ZIF-8 films with biomolecules,which offer the high assay sensitivity and good *** immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex *** clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic *** component analysis(PCA)reveals that 18 patient samples could be fully separated from healthy control *** low-cost,easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases,and can also be generalized to other immunoreaction-based biomarkers detections.

关键词： zeolitic imidazolate framework(ZIF) integrated nanostructure microsensor biomarker detection interleukins

Anti-inflammatory natural products modulate interleukins and their related signaling markers in inflammatory bowel disease:A systematic review

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Journal of Pharmaceutical Analysis 2023年第12期13卷 1408-1428页

作者： Gopalsamy Rajiv Gandhi Thiruchenduran Mohana Kumaraswamy Athesh Varghese Edwin Hillary Alan Bruno Silva Vasconcelos Mariana Nobre Farias de Franca Monalisa Martins Montalvão Stanislaus Antony Ceasar Gnanasekaran Jothi Gurunagarajan Sridharan Ricardo Queiroz Gurgel Baojun Xu Division of Phytochemistry and Drug Design Department of BiosciencesRajagiri College of Social Sciences(Autonomous)KalamaserryKochi683104KeralaIndia Department of Biochemistry Meenakshi Ammal Dental College and Hospital(MAHER)Maduravoyal600095ChennaiTamil NaduIndia Department of Biochemistry Srimad Andavan Arts and Science College(Autonomous)Affiliated to Bharathidasan UniversityTiruchirapalli620005Tamil NaduIndia Division of Plant Molecular Biology and Biotechnology Department of BiosciencesRajagiri College of Social Sciences(Autonomous)KalamaserryKochi683104KeralaIndia Laboratory of Biology and Immunology of Cancer and Leishmania Department of MorphologyFederal University of SergipeSao CristovaoSergipeBrazil Postgraduate Program in Health Sciences(PPGCS) Federal University of Sergipe(UFS)Campus Prof.Joao Cardoso NascimentoAracajuCEP 49060.108SergipeBrazil Programme of Food Science and Technology Department of Life SciencesBNU-HKBU United International CollegeZhuhaiGuangdong519087China

This review aims to identify in vivo studies investigating the potential of plant substances and their natural molecules in managing inflammatory bowel disease(IBD).Specifically,the objective is to examine the impact of these substances on interleukins and other key inflammatory signaling *** articles published up to December 2022 were identified through a search of the PubMed,Scopus,Web of Science,and Embase *** search used keywords including“inflammatory bowel disease”,“medicinal plants”,“natural molecules”,“anti-inflammatory”,and“ulcerative colitis”,and identified 1,878 potentially relevant articles,of which 89 were included in this review after completion of the selection *** study provides preclinical data on natural products(NPs)that can potentially treat IBD,including ulcerative *** main actions of these NPs relate to their effects on nuclear factor kappa B(NF-κβ),the Janus kinase(JAK)/signal transducer and activator of transcription(STAT)signaling pathway,the regulation of T helper 17/regulatory T cells balance,and oxidative *** ability of these NPs to inhibit intestinal inflammation appears to be dependent on lowering levels of the pro-inflammatory cytokines tumor necrosis factor-alpha(TNF-α),interleukin(IL)-1β,and IL-17,via the Jun N-terminal kinase(JNK)1,NF-κβ-p65,and STAT3 *** addition,NPs were shown to reduce oxidative stress and the severity of ulcerative colitis,as well as increase the activity of antioxidant *** actions suggest that NPs represent a promising treatment for IBD,and potentially have greater efficacy and safety than current treatments.

关键词： Crohn's disease Inflammation interleukins Oxidative stress Phytochemicals Ulcerative colitis

interleukins and interleukin receptors in rheumatoid arthritis: Research, diagnostics and clinical implications

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World Journal of Orthopedics 2014年第4期5卷 516-536页

作者： Lili Magyari Dalma Varszegi Erzsebet Kovesdi Patricia Sarlos Bernadett Farago Andras Javorhazy Katalin Sumegi Zsolt Banfai Bela Melegh Department of Medical Genetics University of Pecs 7624 Pecs Hungary Szentagothai Research Centre 7624 Pecs Hungary Department of Dermatology Venereology and Oncodermatology University of Pecs 7624 Pecs Hungary 1st Department of Internal Medicine University of Pecs 7623 Pecs Hungary Institute of Human Physiology and Clinical Experimental Research Semmelweis University 1446 Budapest Hungary Department of Urology University of Pecs 7621 Pecs Hungary

Rheumatoid arthritis(RA) is an autoimmune disease, resulting in a chronic, systemic inflammatory disorder. It may affect many tissues and organs, but it primarily affects the flexible joints. In clinical practice patient care generates many questions about diagnosis, prognosis, and treatment. It is challenging for health care specialists to keep up to date with the medical literature. This review summarizes the pathogenesis, the polymorphisms of interleukin and interleukin genes and the standard available and possible future immunologictargets for RA treatment. The identification of diseaseassociated interleukin and interleukin receptor genes can provide precious insight into the genetic variations prior to disease onset in order to identify the pathways important for RA pathogenesis. The knowledge of the complex genetic background may prove useful for developing novel therapies and making personalized medicine based on the individual's genetics.

关键词： Rheumatoid arthritis interleukins Polymorphisms Immunologic targets Therapy

The dual roles of cytokines in Alzheimer’s disease:update on interleukins, TNF-α,TGF-β and IFN-γ

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Translational Neurodegeneration 2016年第1期5卷 52-66页

作者： Cong Zheng Xin-Wen Zhou Jian-Zhi Wang Department of Pathophysiology School of Basic Medicine and the Collaborative Innovation Center for Brain ScienceKey Laboratory of Ministry of Education of China for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Co-innovation Center of Neuroregeneration Nantong 226000China

Alzheimer’s disease(AD)is one of the most common neurodegenerative disorders in the *** the mechanisms underlying AD neurodegeneration are not fully understood,it is well recognized that inflammation plays a crucial role in the initiation and/or deterioration of AD *** evidence suggests that different cytokines,including interleukins,TNF-α,TGF-βand IFN-γ,are actively participated in AD pathogenesis and may serve as diagnostic or therapeutic targets for AD ***,we review the progress in understanding the important role that these cytokines or neuroinflammation has played in AD etiology and pathogenesis.

关键词： Alzheimer’s disease Cytokines interleukins TNF-α TGF-β IFN-γ

interleukins and Acute Phase Proteins of Bovine Sera during Natural Helminth Burden in Ibadan Nigeria

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Open Journal of Veterinary Medicine 2018年第3期8卷 36-46页

作者： Olalekan Taiwo Jeremiah Gabriel Olamilekan Banwo Department of Veterinary Medicine University of Ibadan Ibadan Nigeria

Inflammatory reactions in the gastrointestinal tract play an important role in the pathogenesis of gastroenteritis in bovine helminthosis. This study determined the serum concentrations of cytokines induced acute phase proteins (Serum Amyloid A (SAA), Haptoglobin (Hp) and C-reactive protein (CRP)) and the levels of immunoreactive interleukins (IL-1, IL-2, IL-3, IL-4, IL-5 and IL-6) in cattle naturally infected with helminths. We sampled a total of 480 slaughtered cattle of both sexes in a major abattoir in Ibadan, Nigeria. Sedimentation, floatation and modified McMaster techniques were employed to determine the degrees of helminth infections. Animals with eggs per gram (epg) less than 200 were adjudged to be apparently healthy. The serum concentrations of interleukins and acute phase proteins were determined using the Technicon AutoAnalyzer Model AA II. It was found that mean SAA (μg/l) and CRP (%) levels were significantly (P 0.05). Therefore, some acute phase proteins are involved in the pathophysiology of bovine helminthosis and are closely related to the inflammatory activation of the disease. In lieu of these findings, it is suggested that systemic markers of inflammation can identify subjects at high risk of natural bovine helminthosis and that IL-6 and SAA may be used as indicators for bovine helminthosis.

关键词： C-Reactive Protein (CRP) interleukins Bovine Helminthosis Haptoglobin Serum Amyloid A (SAA)

interleukins in liver disease treatment

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World Journal of Hepatology 2024年第2期16卷 140-145页

作者： Ming Yang Chun-Ye Zhang Department of Surgery University of MissouriColumbiaMO 65212United States Bond Life Sciences Center University of MissouriColumbiaMO 65212United States

Cytokines play pleiotropic roles in human health and disease by regulating both innate and adaptive immune ***(ILs),a large group of cytokines,can be divided into seven families,including IL-1,IL-2,IL-6,IL-8,IL-10,IL-12,and IL-17 ***,we review the functions of ILs in the pathogenesis and resolution of liver diseases,such as liver inflammation(e.g.,IL-35),alcoholrelated liver disease(e.g.,IL-11),non-alcoholic steatohepatitis(e.g.,IL-22),liver fibrosis(e.g.,Il-17a),and liver cancer(e.g.,IL-8).Overall,IL-1 family members are implicated in liver inflammation induced by different etiologies,such as alcohol consumption,high-fat diet,and hepatitis ***-2 family members mainly regulate T lymphocyte and NK cell proliferation and activation,and the differentiation of T ***-6 family cytokines play important roles in acute phase response in liver infection,liver regeneration,and metabolic regulation,as well as lymphocyte ***-8,also known as CXCL8,is activated in chronic liver diseases,which is associated with the accumulation of neutrophils and ***-10 family members contribute key roles to liver immune tolerance and immunosuppression in liver ***-12 family cytokines influence T-cell differentiation and play an essential role in autoimmune liver ***-17 subfamilies contribute to infection defense,liver inflammation,and Th17 cell *** interact with different type I and type II cytokine receptors to regulate intracellular signaling pathways that mediate their ***,most clinical studies are only performed to evaluate IL-mediated therapies on alcohol and hepatitis virus infection-induced *** pre-clinical and clinical studies are required to evaluate IL-mediated monotherapy and synergistic therapies.

关键词： interleukins Family members Liver disease Treatment Clinical trials

IL-2 and IL-15 Exhibit Opposing Effects on Fas Mediated Apoptosis

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Cellular & Molecular Immunology 2004年第2期1卷 123-128页

作者： Gulcin Demirci Department of Medicine Harvard Medical SchoolDivision of ImmunologyBeth Israel Deaconess Medical CenterBostonMA 02215USA.

It has been shown that IL-2 and IL-15 can have opposing effects on life and death of T ***,the roie of IL-2 and IL-15 in regulating the fate of other cell types is less *** the present study,we examined the impact of IL-2 and IL-15 on life and death of pre-B cells using the BAF-B03 *** showed that BAF-B03 cells constitutively expressed the private IL-2Rα chain and IL-15Rα chain,and the shared IL-2Rβ chain and γc *** of BAF-B03 cells in vitro with IL-2 and IL-15 induced vigorous cell proliferation in a dose-dependent *** of IL-2 and IL-15 in the assay showed that the mitotic effects of IL-2 and IL-15 were remarkably ***,the sensitivities of BAF-B03 cells to Fas mediated apoptosis after IL-2 and IL-15 stimulation were strikingly *** cultured in IL-2 readily underwent apoptotic cell death upon cross-linking of the Fas receptor whereas cells cultured in IL-15 were extremely resistant to Fas triggered cell *** anti-apoptotic effect of IL-15 in this model was associated with increased expression of Bcl-xL. FLIP expression,however,was comparable between IL-2 and IL-15 stimulated *** conclude that IL-2 and IL-15 have diametrically opposite effect on the fate of BAF-B03 cells,although both cytokines share similar receptor structure and exhibit similar mitotic *** & Molecular Immunology.2004;1(2):123-128.

关键词： cytokines interleukins apoptosis lymphocytes

Hydrolyzed seawater pearl tablet modulates the immunity via attenuating Th1/Th2 imbalance in an immunosuppressed mouse model

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Journal of Traditional Chinese Medicine 2021年第3期41卷 397-405页

作者： LAN Taijin LUO Xuefei MO Mingyue CHEN Zhenxing LUO Fei HAN Siyin LIU Peng LIANG Zhongxiu ZHANG Ting LI Tianyi YAN Qiangqiang LIN Yong LIN Jiang School of Basic Medical Science Guangxi University of Chinese MedicineNanning 530200China Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine Nanning 530011China Beihai Baozhulin Marine Technology Co.Ltd. Beihai 536000China

OBJECTIVE: To investigate whether Hydrolyzed Seawater Pearl tablet(HSPT) could modulate the Th1/Th2 imbalance in an immunosuppressed mouse model with Th1 to Th2 shift induced by Cyclosporine A(Cs A) which can be used in the clinical treatment of Th2 to Th1 shift diseases, and explore the possible mechanism for the adjuvant therapeutic efficacy of HSPT on recurrent respiratory infections(RRI) and acquired immune deficiency syndrome(AIDS).METHODS: The mice were randomly divided into six groups of five animals each, namely normal group, model group, lentinan polysaccharide tablet(LPT) group and three HPST treated groups. HPST treated groups were administered with HPST(0.51,1.02, 2.04 g/kg) via intragastric gavage(i.g) for 30 consecutive days. LPT used as reference drug for positive control, LPT group was administered with LPT(8.2 mg/kg) for 30 consecutive days. Normal group and model group were received distilled water. The animals in model group, LPT group and HPST treated groups were injected intraperitoneally with Cs A(50 mg/kg) to establish the immunosuppressed mice model with Th1 to Th2 shift on the20 th, 22 nd and 24 th day, one hour after the administration of the respective treatment. Animals were sacrificed one hour after the last administration to collect blood and splenic tissue. The proportion of T cells including CD8+ and CD4+ T cells, Th1 and Th2 in peripheral blood of experimental mice were measured by flow cytometric. The protein level in serum and m RNA level in splenic tissue of experimental mice for interleukin(IL)-2, IL-12, interferon-γ(IFN-γ), IL-4, IL-6, IL-10 and IL-13 were measured by enzyme linked immunosorbent assay and fluorescence quantitative polymerase chain reaction ***: HSPT elevated the proportion of T cells including both CD8+ and CD4+ T cells, in which the proportion of Th1 and Th2 cells increased, while the ratio of Th1/Th2 cells decreased in peripheral blood of the immunosuppressed mouse model with Th1 to Th2 shift

关键词： respiratory tract infections acquired immunodeficiency syndrome Th1-Th2 balance interleukins interferon-gamma Hydrolyzed Seawater Pearl tablet

Sini powder ameliorates the inflammatory response in rats with stress-induced non-alcoholic fatty liver disease by inhibiting the nuclear factor kappa-B/pyrin domain-containing protein 3 pathway

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Journal of Traditional Chinese Medicine 2020年第2期40卷 253-266页

作者： Mu Jie Cheng Fafeng Wang Qingguo Wang Xueqian Zhu Wenxiang Ma Chongyang Yin Xiangjun Ren Beida Lian Yajun Du Xin Zhang Haixia Liu Shuling Zhang Shuang Teaching and Research Office of Clinical Foundation of Chinese Medicine School of Basic MedicineInnovation Team of"Basic Research on the Application of Classical Formulas"Beijing University of Chinese MedicineBeijing 100029China

OBJECTIVE: To evaluate the effectiveness of Sini powder for the treatment of non-alcoholic fatty liver disease(NAFLD) in rats and the molecular mechanisms ***: A rat model of stress-induced NAFLD was established by a combination of long-term tethering and feeding of a high-fat, high-calorie diet. These rats were then intragastrically administered with either simvastatin, Sini powder, or vehicle for 1 week. The body mass and field test scores for each group were recorded weekly. Serum aspartate aminotransferase and alanine aminotransferase activities, and triglyceride, total cholesterol,and free fatty acid concentrations were *** tissue histopathology was examined on hematoxylin and eosin-stained paraffin sections and oil red O-stained frozen sections. The hepatic m RNA expression of nuclear factor kappa-B(NF-κB),NOD-, LRR-, and pyrin domain-containing protein 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC), and caspase-1 were measured by reverse transcription-polymerase chain reaction(RT-PCR). The hepatic protein concentrations of NF-κB and NLRP3, ASC, caspase-1, interleukin-1β(IL-1β), interleukin-6(IL-6), and the serum concentrations of IL-1β and IL-6 were measured by enzyme-linked immunosorbent ***: Compared with the Blank group, rats in the Compound model group showed significant pathologic manifestations of NAFLD, and the expression of NF-κB, NLRP3, ASC, caspase-1, IL-1βand IL-6 were significantly higher(all P < 0.01). Both simvastatin and Sini powder significantly ameliorated the NAFLD pathology and the abnormal expression of NF-κB, NLRP3, ASC, caspase-1, IL-1β, and IL-6(all P < 0.01).CONCLUSION: Sini powder inhibits the inflamma-tory response in rats with NAFLD, which is mediated by NF-κB/NLRP3, IL-1β, and IL-6, reduces the effects of psychological stress, and improves lipid metabolism. Therefore, Sini powder may be effective for the treatment of stress-related NAFLD through multiple mechanisms.

关键词： Non-alcoholic fatty liver Stress,psychological NLR family,pyrin domain-containing 3 protein NF-kappa B interleukins Sini powder