检索结果-南通市图书馆

Effect of tubastatin A on NLRP3 inflammasome activation in macrophages under hypoxia/ reoxygenation conditions

World Journal of Emergency Medicine 2024年第4期15卷 289-296页

作者： Hao Li Chang Liu Ying Cui Panpan Chang Wei chong Department of Emergency Medicine the First Hospital of China Medical UniversityShenyang 110001China Trauma Medicine Center Peking University People's HospitalBeijing 100871China Key Laboratory of Trauma and Neural Regeneration Peking UniversityBeijing 100871China National Center for Trauma Medicine of China Beijing 100871China

BACKGROUND:There are currently no effective drugs to mitigate the ischemia/reperfusion injury caused by fluid resuscitation after hemorrhagic shock(HS).The aim of this study was to explore the potential of the histone deacetylase 6(HDAC6)-specific inhibitor tubastatin A(TubA)to suppress nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation in macrophages under hypoxia/reoxygenation(H/R)***:The viability of RAW264.7 cells subjected to H/R after treatment with different concentrations of TubA was assessed using a cell-counting kit-8(CCK8)***,2.5μmol/L TubA was used with RAW264.7 cells under H/R ***264.7 cells were divided into three groups,namely the control,H/R,and TubA *** levels of reactive oxygen species(ROS)in the cells were detected using fluorescence *** protein expression of HDAC6,heat shock protein 90(Hsp90),inducible nitric oxide synthase(iNOS),NLRP3,gasdermin-D(GSDMD),Caspase-1,GSDMD-N,and Caspase-1 p20 was detected by western *** levels of interleukin-1β(il-1β)and il-18 in the supernatants were detected using enzyme-linked immunosorbent assay(ELISA).RESULTS:HDAC6,Hsp90,and iNOS expression levels were significantly higher(Pil-1βand il-18 levels in the supernatants were significantly higher in the H/R group compared to the control group(P<0.01),but significantly lower in the TubA group compared to the H/R group(P<0.01).CONCLUSION:TubA inhibited the expression of HDAC6,Hsp90,and iNOS in macrophages

关键词： Hemorrhagic shock Hypoxia/reoxygenation Macrophage NLRP3 Tubastatin A

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of moxibustion on Treg cells in sarcoma microenvironment

引用

Journal of Integrative Medicine 2021年第3期19卷 251-257页

作者： Li-jun Li Yu-chong Shi Min-xiang Luo Chang-lin Zhao Department of Acupuncture Guangdong Clifford HospitalGuangzhou 511495Guangdong ProvinceChina School of Traditional Chinese Medicine Jinan UniversityGuangzhou 510632Guangdong ProvinceChina Health Science College Guangdong Pharmaceutical UniversityGuangzhou 510006Guangdong ProvinceChina

Objective:To investigate the therapeutic effect of moxibustion on sarcomas from mesenchymal tissues,which have a low response rate to chemotherapy and ***:S180 sarcoma cell line was inoculated in C57 BL/6 mice to form transplanted *** therapy was directly applied at the transplanted tumor sites,at a distance of 3.0 cm,10 min per session,till skin temperature reached 45°C,once a day,for 14 consecutive days of *** the mice were killed,serum was collected and used to detect concentrations of interleukin-10(il-10),transforming growth factor-b1(TGF-b1),il-4 and interferon-c(IFN-c)by Luminex liquid suspension *** numbers of Treg^(^(+) )T cells and CD4^(+) CD25^(+) Forkhead Box P3(Foxp3)^(+) T cells were detected by flow *** in situ hybridization was used to analyze the changes of CD4,CD8,Foxp3 and TGF-b1 in the tumor microenvironment(TME).Results:Weight of S180 transplanted tumor in the control group was(2.03±0.54)g,and that in the moxibustion group was(1.27±0.29)g,which was statistically different(P=0.023).The mean value of Foxp3^(+) T cells in the normal group was 2.01%,which increased to 3.63%after the formation of transplanted tumor,and decreased to 1.48%after moxibustion *** moxibustion group also had reduced numbers of CD4^(+) CD25^(+) Foxp3^(+) T cells in the spleen of mice with transplanted *** concentrations of il-10,TGF-b1 and il-4 decreased in the serum of mice with transplanted tumor,while the concentration of IFN-c *** was associated with downregulation in expression of Foxp3,il-10 and TGF-b1 genes in the transplanted tumor,and increases in the gene expression of CD4^(+) T cells and CD8^(+) T cells in the ***:Moxibustion may have therapeutic effects on sarcomas by reducing the number of Treg cells in the blood and controlling the infiltration of Treg cells in the TME.

关键词： Sarcoma Moxibustion Treg cells Tumor microenvironment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

异钩藤碱减轻帕金森病小鼠多巴胺能神经元损伤的作用机制

引用

安徽医药 2025年第1期29卷 23-28页

作者：刘崇刘亚婷张壮周国平南阳市第一人民医院神经内二科河南南阳473000 南阳市中心医院神经外科河南南阳473000

目的探讨异钩藤碱对帕金森病(PD)小鼠多巴胺能神经元损伤及凋亡的影响及其可能作用机制。方法2020年6月至2022年6月选取40只小鼠建立PD模型,造模成功小鼠按照随机数字表法分为模型组、异钩藤碱低、中和高剂量组,每组各10只,另设对照组1... 详细信息

目的探讨异钩藤碱对帕金森病(PD)小鼠多巴胺能神经元损伤及凋亡的影响及其可能作用机制。方法2020年6月至2022年6月选取40只小鼠建立PD模型,造模成功小鼠按照随机数字表法分为模型组、异钩藤碱低、中和高剂量组,每组各10只,另设对照组10只。异钩藤碱低、中和高剂量组小鼠分别灌胃给药20、40、80 mg/kg的异钩藤碱混悬液(0.9%氯化钠溶液配制),对照组及模型组给予等量0.9%氯化钠溶液。采用爬杆实验和旷场实验评定各组小鼠行为学变化;ELISA法检测小鼠血清中白细胞介素-1β(il-1β)和肿瘤坏死因子α(TNF-α)水平以及脑组织中多巴胺(DA)水平;TUNEL法检测黑质区神经细胞凋亡;逆转录聚合酶链式反应(RT-PCR)法检测黑质区微RNA-376b-3p(miR-376b-3p)、转录因子SP1(SP1)和亮氨酸重复序列激酶2(LRRK2)的mRNA相对表达量;蛋白质印迹法检测黑质区SP1、LRRK2、α-突触核蛋白(α-syn)和酪氨酸羟化酶(TH)蛋白表达情况。结果与对照组(4.58±0.27)μg/L比较,模型组(1.35±0.13)μg/L小鼠DA水平显著降低(Pil-1β、TNF-α水平、神经细胞凋亡率、SP1和LRRK2 mRNA和蛋白表达水平、α-syn蛋白水平降低,运动总距离、miR-376b-3p以及TH蛋白水平升高(P<0.05),且以异钩藤碱高剂量组效果最佳(P<0.05)。结论异钩藤碱能够在一定程度上改善PD小鼠运动功能障碍,减轻神经炎症反应,降低神经细胞凋亡率,可能是通过影响miR-376b-3p/SP1/LRRK2信号系统发挥作用。

关键词：钩藤属帕金森病异钩藤碱神经元损伤转录因子SP1 亮氨酸重复序列激酶2

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A bifunctional and recyclable catalyst:Amine and ionic liquid grafting on MOFs for the one-pot synthesis of N-aryl oxazolidin-2-ones

引用

Green Energy & Environment 2020年第2期5卷 154-165页

作者： Siying chong Tongtong Wang Haijun Zhong Lingfei Xu Hailong Xu Zhongwu Lv Min Ji Department of Chemistry School of Chemical EngineeringDalian University of TechnologyDalian116023China Petrochemical Research Institute China National Petroleum CorporationBeijing112206China

A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/il–*** structure and physical-chemical characterization of DMEDA/il–NH2-Mil-101 and its precursors were characterized by SEM,N2 adsorption-desorption,XPS,FT-IR,PXRD,elemental analysis,and TGA *** date showed that the two catalytic components of N,N-dimethylethylenediamine(DMEDA)and 1-butyl-3-methylimidazolium bromide(BmimBr)were chemically immobilized in NH2-Mil-101 *** amine of DMEDA was grafted onto carrier NH2-Mil-101 by N–Cr coordinate covalent bonds and the ionic liquid of BmimBr(il(Br-))was anchored in the NH2-Mil-101 nanocages by'ship-in-a-bottle'method,in which the amidogen of NH2-Mil-101 condensed with N,N-carbonyldiimidazole(CDI)firstly,and then alkylated with 1-bromo *** novel heterogeneous catalyst with two different active sites can efficiently catalyze the synthesis of N-aryl oxazolidin-2-ones from carbon dioxide(CO2),epoxides,and anilines in one-pot under mild solvent-free *** not only showed good stability and recoverability after five cycles but also exhibited shape selectivity for the substrate due to the synergic catalysis of amine,ionic liquid,and *** novel bifunctional material is a promising solid catalyst for the green synthesis of N-aryl oxazolidin-2-ones.

关键词： Metal-organic frameworks Ionic liquid immobilization N-aryl oxazolidin-2-ones One-pot synthesis Bifunctional heterogeneous catalyst

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The pursuit of transplantation tolerance:new mechanistic insights

引用

Cellular & Molecular Immunology 2019年第4期16卷 324-333页

作者： Pawan K.Gupta Christine M.McIntosh Anita S.chong Maria-Luisa Alegre Department of Medicine The University of ChicagoChicagoIL 60637USA Department of Surgery The University of ChicagoChicagoIL 60637USA

Donor-specific transplantation tolerance that enables weaning from immunosuppressive drugs but retains immune competence to non-graft antigens has been a lasting pursuit since the discovery of neonatal *** recently,efforts have been devoted not only to understanding how transplantation tolerance can be induced but also the mechanisms necessary to maintain it as well as how inflammatory exposure challenges its *** review focuses on recent advances regarding key peripheral mechanisms of T cell tolerance,with the underlying hypothesis that a combination of several of these mechanisms may afford a more robust and durable tolerance and that a better understanding of these individual pathways may permit longitudinal tracking of tolerance following clinical transplantation to serve as *** review may enable a personalized assessment of the degree of tolerance in individual patients and the opportunity to strengthen the robustness of peripheral tolerance.

关键词： tolerance TCR avidity Treg T cell dysfunction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tacrolimus for children with refractory nephrotic syndrome:a one-year prospective,multicenter,and open-label study of Tacrobell®,a generic formula

引用

World Journal of Pediatrics 2016年第1期12卷 60-65页

作者： Eun Mi Yang Sang Taek Lee Hyun Jin Choi Hee Yeon Cho Joo Hoon Lee Hee Gyung Kang Young Seo Park Hae il Cheong il-Soo Ha Department of Pediatrics Chonnam National University HospitalGawngjuKorea Department of Pediatrics Seoul National University Children's HospitalSeoulKorea Department of Pediatrics Samsung Medical CenterSungkynkwan University School of MedicineSeoulKorea Department of Pediatrics Asan Medical Center Children's HospitalUniversity of Ulsan College of MedicineSeoulKorea Research Coordination Center for Rare Diseases Seoul National University HospitalSeoulKorea Kidney Research Institute Medical Research CenterSeoul National University College of MedicineSeoulKorea

Background:Cyclosporine A and tacrolimus(TAC)are often used as a second-line treatment for children with refractory nephrotic syndrome(NS).This study was undertaken to investigate the efficacy and safety of Tacrobell®,a locally produced generic form of ***:This study was a one-year prospective,open-label,single-arm,multicenter ***-four children with steroid-dependent NS(SDNS)and 33 children with steroid-resistant NS(SRNS)were *** primary endpoints were defined as the remission rates,whereas the secondary endpoints were recognized as the duration of remission and adverse effects of ***:After one-year treatment,34(77.3%)of the 44 patients with SDNS were in complete remission,and 6(13.6%)were in partial ***(43.2%)patients did not relapse during the study;for those who did relapse,the mean duration of remission was 4.6±2.9 *** number of relapse episodes during the study period(0.90 per patient-year)was significantly lower than that in the preceding year(2.8 per patientyear).After treatment for 3 and 6 months,12(36.4%)of the 33 patients with SRNS were in remission,and after treatment for 12 months,the number of patients had increased to 13(39.4%).The mean time to achieve remission was 4.0±3.2 *** remission(duration,3.7±2.7 months),12(54.5%)of 22 patients *** fasting blood glucose and blood pressure levels during the therapy were similar to those at the time of study ***:Treatment with Tacrobell®was effective and safe for children with refractory *** efficacy of this generic form of TAC was better than that of the original TAC formula.

关键词： generic drugs nephrotic syndrome tacrolimus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Changes in intestinal mucosal immune barrier in rats with endotoxemia

引用

World Journal of Gastroenterology 2009年第46期15卷 5843-5850页

作者： chong Liu Ang Li Yi-Bing Weng Mei-Li Duan Bao-En Wang Shu-Wen Zhang Departmentof Critical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China Liver Research Center Beijing FriendshipHospital Affiliated to Capital Medical University Beijing100050 China Department of Infectious Disease andCritical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China

AIM： To investigate the dysfunction of the immunological barrier of the intestinal mucosa during endotoxemia and to elucidate the potential mechanism of this dysfunction. METHODS： Male Wistar rats were randomly distributed into two groups： control group and lipopolysaccharide （LPS） group. Endotoxemia was induced by a single caudal venous injection of LPS. Animals were sacrificed in batches 2, 6, 12 and 24 h after LPS infusion. The number of microfold （M）-cells, dendritic cells （DCs）, CD4＋ T cells, CD8＋ T cells, regulatory T （Tr） cells and IgA＋ B cells in the intestinal mucosa were counted after immunohistochemical staining. Apoptotic lymphocytes were counted after TUNEL staining. The levels of interleukin （il）-4, interferon （IFN）-γ, and forkhead box P3 （Foxp3） in mucosal homogenates were measured by ELISA. The secretory IgA （sIgA） content in the total protein of one milligram of small intestinal mucus was detected using a radioimmunological ***： This research demonstrated that LPS-induced endotoxemia results in small intestinal mucosa injury. The number of M-cells, DCs, CD8~ T cells, and IgA~ B cells were decreased while Tr cell and apoptotic lymphocyte numbers were increased significantly. The number of CD4＋ T cells increased in the early stages and then slightly decreased by 24 h. The level of il-4 significantly increased in the early stages and then reversed by the end of the study period. The level of IFN-T increased slightly in the early stages and then decreased markedly by the 24 h time point. Level of Foxp3 increased whereas sIgA level ***： Mucosal immune dysfunction forms part of the intestinal barrier injury during endotoxemia. The increased number and function of Tr cells as well as lymphocyte apoptosis result in mucosal immunode- ficiency.

关键词： Endotoxemia Rats Intestinal mucosa Immunity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice

引用

Current Medical Science 2020年第4期40卷 729-738页

作者： Fa-yuan LIU Peng BAI Ye-fan JIANG Nian-guo DONG Geng LI chong CHU Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Summary:Interleukin 17A(il 17A)is reported to be involved in many inflammatory processes,but its role in aortic valve diseases remains *** examined the role of il17A based on an ApoE^-/-mouse model with strategies as fed with high-fat diet or treated with ilI7A monoclonal antibody(mAb).12 weeks of high-fat diet feeding can elevate cytokines secretion,inflammatory cells infiltration and myofibroblastic transition of valvular interstitial cells(VICs)in aortic ***,diet-induction accelerated interleukin 17 receptor A(il17RA)activation in *** an il17A inhibition model,the treatment group was intra-peritoneally injected with anti-il17A mAb while controls received irrelevant *** blockade of il17A markedly reduced cellular infiltration and transition in aortic *** investigate potential mechanisms,NF-kB was co-stained in il17RA^+VICs and il17RA macrophages,and further confirmed by Western blotting in ***-fat diet could activate NF-kB nuclear translocation in il17RA^+VICs and il17RA^+macrophages and this process was depressed after il17A *** conclusion,high-fat diet can lead to il17A upregulation,VICs myofibroblastic transition and inflammatory cells infiltration in the aortic value of ApoE^-/-*** il17A with il17A mAb can alleviate aortic valve inflammatory states.

关键词： aortic valve inflammation interleukin 17A NF-kB pathway intensity correlation analysis colocalization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mesenchymal stem cells rescue acute hepatic failure by polarizing M2 macrophages

引用

World Journal of Gastroenterology 2017年第45期23卷 7978-7988页

作者： yan-wei li chong zhang qiu-ju sheng han bai yang ding xiao-guang dou department of infectious diseases shengjing hospital of china medical university

AIM To investigate whether M1 or M2 polarization contributes to the therapeutic effects of mesenchymal stem cells(MSCs) in acute hepatic failure(AHF).METHODS MSCs were transfused into rats with AHF induced by D-galactosamine(DGal N). The therapeutic effects of MSCs were evaluated based on survival rate and hepatocyte proliferation and apoptosis. Hepatocyte regeneration capacity was evaluated by the expression of the hepatic progenitor surface marker epithelial cell adhesion molecule(Ep CAM). Macrophage polarization was analyzed by M1 markers [CD68,tumor necrosis factor alpha(TNF-α),interferon-γ(IFN-γ),inducible nitric oxide synthase(INOS)] and M2 markers [CD163,interleukin(il)-4,il-10,arginase-1(Arg-1)] in the survival and death groups after MSC *** The survival rate in the MSC-treated group was increased compared with the DPBS-treated control group(37.5% vs 10%). MSC treatment protected rats with AHF by reducing apoptotic hepatocytes and promoting hepatocyte regeneration. Immunohistochemical analysis showed that MSC treatment significantly increased the expression of Ep CAM compared with the control groups(P il-10 and Arg-1 in the survival group. il-4 concentrations were significantly increased compared to the death group after MSC transplantation(88.51 ± 24.51 pg/m L vs 34.61 ± 6.6 pg/m L,P < 0.001). In contrast,macrophages showed strong expression of CD68,TNF-α,and INOS in the death group. The concentration of IFN-γ was significantly increased compared to the survival group after MSC transplantation(542.11 ± 51.59 pg/m L vs 104.07 ± 42.80 pg/m L,P < 0.001).CONCLUSION M2 polarization contributes to the therapeutic effects of MSCs in AHF by altering levels of anti-inflammatory and pro-infla

关键词： Acute hepatic failure Mesenchymal stem cells Macrophages Polarization Inflammation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study on the Effects of Cytokine Level in Ducklings with Selenium Poisoning

引用

Agricultural Sciences in China 2007年第3期6卷 375-380页

作者： WU Rui LIAN Xue-zhao TANG Xiao-yan FU Yan-ling HE chong-yi College of Animal Science and Technology Heilongjiang August First Land Reclamation University Daqing 163319 P.R.China

One-day-old healthy ducklings （n = 100） were randomly allocated into two groups （n = 50, respectively） to investigate the effect of cytokines on ducklings with selenium poisoning. The ducklings in control group （Group Ⅰ） were fed with full rations containing 0.3 mg kg^-1 selenium, and the ducklings in selenium poisoning group （Group Ⅱ ） were fed with seleniumexcessive rations containing 8.0 mg kg^-1 selenium. One mL blood was collected by cardiac puncture at d 15, 30, 60, and 90 （n = 10, respectively）, serum was separated by centrifugation. Contents of cytokines were determined by the radioimmunity method （RIA）. The results showed that the contents of il-113, il-213 in Group Ⅱ were higher than that in control group at d 15 （P〉0.05）, but were significantly lower at d 30, 60, and 90 （P〈0.01）; the contents of il-8β in Group II were significantly lower than that in control group （P〈0.01）; the contents of erythropoietin （EPO） and IGF-2 in Group II were significantly lower in different degree than that in control group at d 60 and 90 （P〈0.01）; the contents of granulocyte- macrophage colony stimulating factor （GM-CSF） in the two groups were lower in different degree and significantly lower at d 60 and 90 （P〈0.01）. It can be concluded that the excessive selenium could result in a decrease in immunity and a series of clinical pathology changes, such as anemia, tissue hemorrhage, and necrosis.

关键词： ducklings selenium poisoning cytokine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：