Changes in intestinal mucosal immune barrier in rats with endotoxemia
Changes in intestinal mucosal immune barrier in rats with endotoxemia作者机构：Departmentof Critical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China Liver Research Center Beijing FriendshipHospital Affiliated to Capital Medical University Beijing100050 China Department of Infectious Disease andCritical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2009年第15卷第46期
页 面：5843-5850页
核心收录：
学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 1002[医学-临床医学] 07[理学] 09[农学] 0906[农学-兽医学] 071002[理学-动物学]
基 金：Supported by Beijing Municipal Science & Technology Commission Major Scitech Program No.H020920050130
主 题：Endotoxemia Rats Intestinal mucosa Immunity
摘 要：AIM： To investigate the dysfunction of the immunological barrier of the intestinal mucosa during endotoxemia and to elucidate the potential mechanism of this dysfunction. METHODS： Male Wistar rats were randomly distributed into two groups： control group and lipopolysaccharide （LPS） group. Endotoxemia was induced by a single caudal venous injection of LPS. Animals were sacrificed in batches 2, 6, 12 and 24 h after LPS infusion. The number of microfold （M）-cells, dendritic cells （DCs）, CD4＋ T cells, CD8＋ T cells, regulatory T （Tr） cells and IgA＋ B cells in the intestinal mucosa were counted after immunohistochemical staining. Apoptotic lymphocytes were counted after TUNEL staining. The levels of interleukin （IL）-4, interferon （IFN）-γ, and forkhead box P3 （Foxp3） in mucosal homogenates were measured by ELISA. The secretory IgA （sIgA） content in the total protein of one milligram of small intestinal mucus was detected using a radioimmunological ***： This research demonstrated that LPS-induced endotoxemia results in small intestinal mucosa injury. The number of M-cells, DCs, CD8~ T cells, and IgA~ B cells were decreased while Tr cell and apoptotic lymphocyte numbers were increased significantly. The number of CD4＋ T cells increased in the early stages and then slightly decreased by 24 h. The level of IL-4 significantly increased in the early stages and then reversed by the end of the study period. The level of IFN-T increased slightly in the early stages and then decreased markedly by the 24 h time point. Level of Foxp3 increased whereas sIgA level ***： Mucosal immune dysfunction forms part of the intestinal barrier injury during endotoxemia. The increased number and function of Tr cells as well as lymphocyte apoptosis result in mucosal immunode- ficiency.
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