Effects of moxibustion on Treg cells in sarcoma microenvironment

作     者：Li-jun Li Yu-chong Shi Min-xiang Luo Chang-lin Zhao Li-jun Li;Yu-chong Shi;Min-xiang Luo;Chang-lin Zhao

作者机构：Department of AcupunctureGuangdong Clifford HospitalGuangzhou 511495Guangdong ProvinceChina School of Traditional Chinese MedicineJinan UniversityGuangzhou 510632Guangdong ProvinceChina Health Science CollegeGuangdong Pharmaceutical UniversityGuangzhou 510006Guangdong ProvinceChina 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2021年第19卷第3期

页      面：251-257页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by the Major Science and Technology Project of Guangzhou Panyu District(No.2017-z04-01) Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine(No.20182119) Science and Technology Project of Health and Family Planning in Guangzhou(No.20192A011028) 

主　　题：Sarcoma Moxibustion Treg cells Tumor microenvironment 

摘      要：Objective:To investigate the therapeutic effect of moxibustion on sarcomas from mesenchymal tissues,which have a low response rate to chemotherapy and ***:S180 sarcoma cell line was inoculated in C57 BL/6 mice to form transplanted *** therapy was directly applied at the transplanted tumor sites,at a distance of 3.0 cm,10 min per session,till skin temperature reached 45°C,once a day,for 14 consecutive days of *** the mice were killed,serum was collected and used to detect concentrations of interleukin-10(IL-10),transforming growth factor-b1(TGF-b1),IL-4 and interferon-c(IFN-c)by Luminex liquid suspension *** numbers of Treg^(^(+) )T cells and CD4^(+) CD25^(+) Forkhead Box P3(Foxp3)^(+) T cells were detected by flow *** in situ hybridization was used to analyze the changes of CD4,CD8,Foxp3 and TGF-b1 in the tumor microenvironment(TME).Results:Weight of S180 transplanted tumor in the control group was(2.03±0.54)g,and that in the moxibustion group was(1.27±0.29)g,which was statistically different(P=0.023).The mean value of Foxp3^(+) T cells in the normal group was 2.01%,which increased to 3.63%after the formation of transplanted tumor,and decreased to 1.48%after moxibustion *** moxibustion group also had reduced numbers of CD4^(+) CD25^(+) Foxp3^(+) T cells in the spleen of mice with transplanted *** concentrations of IL-10,TGF-b1 and IL-4 decreased in the serum of mice with transplanted tumor,while the concentration of IFN-c *** was associated with downregulation in expression of Foxp3,IL-10 and TGF-b1 genes in the transplanted tumor,and increases in the gene expression of CD4^(+) T cells and CD8^(+) T cells in the ***:Moxibustion may have therapeutic effects on sarcomas by reducing the number of Treg cells in the blood and controlling the infiltration of Treg cells in the TME.