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Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii

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Food Bioscience 2022年第3期47卷 1337-1350页

作者： Kouadio Ibrahime Sinan Gokhan Zengin Antonio Fiorentino Chiara Vuotto Mohamad Fawzi Mahomoodally Sharmeen Jugreet Jasmina Glamočlija Marina Soković Ouattara Katinan Etienne catarina guerreiro pereira Luísa Custódio Kannan R.R.Rengasamy Brigida D'Abrosca Department of Biology Science FacultySelcuk UniversityKonyaTurkey Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche –DiSTABiF Università degli Studi della Campania “Luigi Vanvitelli”via Vivaldi 43 I-81100CasertaItaly Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusRéduit80837Mauritius Laboratory of Mycology Department of Plant PhysiologyInstitute for Biological Research “Siniša Stanković”National Institute of Republic of SerbiaUniversity of BelgradeBelgradeSerbia Laboratoire de Botanique UFR BiosciencesUniversité Félix Houphouët-BoignyAbidjanCote d'Ivoire Centre of Marine Sciences University of AlgarveCampus de Gambelas8005-139FaroPortugal Centre for Transdisciplinary Research Department of PharmacologySaveetha Dental CollegeSaveetha Institute of Medical and Technical Sciences (SIMATS)Chennai600077India

Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy,especially in African *** the present study,ethyl acetate,methanolic and water extracts of different parts of (fruits,leaves,stem barks) *** were evaluated for their bioactive contents,enzyme inhibitory,antioxidant and antimicrobial *** potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g),radical scavenging assays (DPPH:20.39–471.28 mg TE/g;ABTS:42.43–654.14 mg TE/g);reducing assays (CUPRAC:102.98–1207.42 mg TE/g;FRAP:45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g).In particular,the methanolic and water extracts showed better antioxidant *** for a few,the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g),butyrylcholinesterase (0.83–13.80 mg GALAE/g),tyrosinase (4.03–143.86 mg KAE/g),amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g).Additionally,all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration:0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration:0.04–0.61 mg/ml) ***,the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.

关键词： Piliostigma thonningii Bioactivities Enzyme inhibitors Antioxidants Antimicrobial NMR Metabolic profiling

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Association of donor hepatectomy time with liver transplantation outcomes: A multicenter retrospective study

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World Journal of Transplantation 2024年第1期14卷 130-140页

作者： Geisiane Custodio Andrew Maykon Massutti Aline Caramori Taynara Gonçalves pereira Augusto Dalazen Gabriela Scheidt Ludmilla Thomazini Cristiane Bauermann Leitão Tatiana Helena Rech Department of Intensive Care Unit Hospital Santa IsabelBlumenau-Santa Catarina CEP-89010906Brazil Transplant Division Hospital Santa IsabelBlumenau-Santa Catarina CEP-89010906Brazil School of Medicine Universidade Regional de Blumenau(FURB)Blumenau-Santa Catarina CEP-89010906Brazil School of Medicine Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Brazil Departement of Endocrinology Hospital de Clínicas de Porto Alegre/Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Department of Internal Medicine Hospital de Clínicas de Porto AlegreDivision of Intensive Care MedicinePorto Alegre 90035-903Brazil

BACKGROUND Prolonged donor hepatectomy time may be implicated in early and late complications of liver *** To evaluate the impact of donor hepatectomy time on outcomes of liver transplant recipients,mainly early allograft *** This multicenter retrospective study included brain-dead donors and adult liver graft ***-recipient matching was obtained through a crossover *** and laboratory data were recorded for both donors and *** hepatectomy,cold ischemia,and warm ischemia times were *** outcome was early allograft *** outcomes included need for retransplantation,length of intensive care unit and hospital stay,and patient and graft survival at 12 *** From January 2019 to December 2021,a total of 243 patients underwent a liver transplant from a brain-dead *** these,57(25%)developed early allograft *** median donor hepatectomy time was 29(23–40)*** with early allograft dysfunction had a median hepatectomy time of 25(22–38)min,whereas those without it had a median time of 30(24–40)min(P=0.126).CONCLUSION Donor hepatectomy time was not associated with early allograft dysfunction,graft survival,or patient survival following liver transplantation.

关键词： Brain death Hepatectomy Liver transplantation Early allograft dysfunction Graft survival

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Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants

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Bioactive Materials 2022年第6期7卷 169-184页

作者： Wei Jiang Fushan Hou Yong Gu Qimanguli Saiding Pingping Bao Jincheng Tang Liang Wu Chunmao Chen Cailiang Shen catarina Leite pereira Marco Sarmento Bruno Sarmento Wenguo Cui Liang Chen Department of Orthopaedic Surgery Orthopedic InstituteThe First Affiliated Hospital of Soochow University708 Renmin RdSuzhouJiangsu215006PR China Department of Orthopedics The Second Hospital of Shanxi Medical UniversityNo.382 Wuyi RoadTaiyuanShanxi030001PR China Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai200025PR China Department of Electrocardiography The First Affiliated Hospital of Anhui Medical University218 Jixi RoadShushan DistrictHefeiAnhui230022PR China Department of Orthopedics The First Affiliated Hospital of Anhui Medical University218 Jixi RoadShushan DistrictHefeiAnhui230022PR China i3S-Instituto de Investigacao e Inovacao em Saude and INEB-dica Universidade do PortoRua Alfredo Allen 2084200-393PortoPortugal CESPU-Institute for Research and Advanced Training in Health Sciences and Technologies Rua Central de Gandra 13174585-116GandraPortugal Faculty of Medicine Universidade de LisboaAvenida Professor Egas Moniz1649-028LisboaPortugal

The effective osteointegration of orthopedic implants is a key factor for the success of orthopedic ***,local metabolic imbalance around implants under osteoporosis condition could jeopardize the fixation *** by the bone structure and the composition around implants under osteoporosis condition,alendronate(A)was grafted onto methacryloyl hyaluronic acid(H)by activating the carboxyl group of methacryloyl hyaluronic acid to be bonded to inorganic calcium phosphate on trabecular bone,which is then integrated with aminated bioactive glass(AB)modified by oxidized dextran(O)for further adhesion to organic collagen on the trabecular *** hybrid hydrogel could be solidified on cancellous bone in situ under UV irradiation and exhibits dual adhesion to organic collagen and inorganic apatite,promoting osteointegration of orthopedic implants,resulting in firm stabilization of the implants in cancellous bone *** vitro,the hydrogel was evidenced to promote osteogenic differentiation of embryonic mouse osteoblast precursor cells(MC3T3-E1)as well as inhibit the receptor activator of nuclear factor-κB ligand(RANKL)-induced osteoclast differentiation of macrophages,leading to the upregulation of osteogenic-related gene and protein *** a rat osteoporosis model,the bone-implant contact(BIC)of the hybrid hydrogel group increased by 2.77,which is directly linked to improved mechanical stability of the orthopedic ***,this organic-inorganic,dual-adhesive hydrogel could be a promising candidate for enhancing the stability of orthopedic implants under osteoporotic conditions.

关键词： Hydrogels Osteoporosis Osseointegration Dual-functional Peri-implant

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Dehydrated Natural Feed for Cats: Influence on Apparent Digestibility Coefficient, Feces Scores, and Serum Immune, Antioxidant, and Metabolic Biomarkers

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Food and Nutrition Sciences 2024年第7期15卷 594-611页

作者： Bruno Giorgio de Oliveira Cécere Guilherme Luiz Deolindo Bianca F. Bissacotti Priscila Marquezin Copetti Maria Rosa Chitolina Schetinger Bruna Klein Matheus Delamea Baldissera Thiago pereira Ribeiro Walter Hugo Cuelho Suarez Daniel Pigatto Monteiro Aleksandro Schafer Da Silva Graduate Multicenter Program in Biochemistry and Molecular Biology University of the State of Santa Catarina Lages Brazil Department of Biochemistry and Molecular Biology Universidade Federal de Santa Maria (UFSM) Santa Maria Brazil Department of Animal Science Universidade Federal de Santa Maria (UFSM) Chapec Brazil Department of Health Science Universidade Franciscana (UFN) Santa Maria Brazil Department of Animal Nutrition Tectron-Tecnologia e Inovao Toledo Brazil

The present study aimed to determine whether natural dehydrated feed (DNF) can replace dry extruded feed while preserving the required daily intake, nutrient digestibility and benefits for immunity and antioxidant response in cats. The animals were divided into two groups of seven animals each. One group received DNF, and the other consumed commercial extruded dry feed (DCF). The experiment lasted 45 days and we measured daily consumption and collected blood and feces samples. The animals in the DNF group showed greater digestibility of fiber and mineral matter than DCF. Cats in the DNF group had a higher volume of feces. DNF cats had lower leukocyte and granulocyte counts and cholesterol levels. Serum enzymatic antioxidant responses and non-enzymatic were higher in DNF cats. Cats in the DNF group showed lower serum acute phase proteins in addition to a tendency toward a higher concentration of transferrin than in the DCF group. Fecal Escherichia coli and total coliform counts of cats fed DNF were higher than DCF. We conclude that the daily consumption of cats is adequate. This feed had greater digestibility of fiber and mineral matter, in addition to an improvement in antioxidant and immunological responses.

关键词： Alternative Feed Antioxidant Companion Animals Health

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Low-grade mucinous appendiceal neoplasm mimicking an ovarian lesion: A case report and review of literature

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World Journal of Clinical Cases 2021年第10期9卷 2334-2343页

作者： André Luís Borges catarina Reis-de-Carvalho Martinha Chorão Helena pereira Dusan Djokovic Department of Obstetrics and Gynecology Hospital de São Francisco Xavier-Centro Hospitalar Lisboa OcidentalLisbon 1449-005Portugal Faculdade de Ciências da Saúde Universidade da Beira InteriorCovilhã6201-001Portugal Department of Obstetrics Gynecology and Reproductive MedicineHospital de Santa Maria-Centro Hospitalar Universitário Lisboa NorteLisbon 1649-028Portugal Department of Pathology Hospital Egas MonizCentro Hospitalar Lisboa OcidentalLisbon 1349-019Portugal Department of Obstetrics and Gynecology Maternidade Dr.Alfredo da Costa-Centro Hospitalar Universitário de Lisboa CentralLisbon 2890-495Portugal Faculdade de Ciências Médicas Nova Medical SchoolLisbon 1169-056Portugal

BACKGROUND Appendiceal tumors are rare lesions that may not be easily differentiated from primary ovarian lesions preoperatively,despite the use of advanced diagnostic methods by experienced *** SUMMARY A 59-year-old G2P2 woman,with chronic pelvic pain,underwent a pelvic ultrasound that revealed an adnexal mass measuring 58 mm×34 mm×36 mm,with irregular borders,heterogeneous echogenicity,no color Doppler vascularization and without acoustic *** ovarian tissue was visualized in contact with the lesion,and it was impossible to separate the lesion from the ovary by applying pressure with the ultrasound ***,peritoneal metastases or other alterations were not *** the international ovarian tumor analysis ADNEX model,the lesion was classified as a malignant tumor(the risk of malignancy was 27.1%,corresponding to Ovarian-Adnexal Reporting Data System category 4).Magnetic resonance imaging confirmed the presence of a right adnexal mass,apparently an ovarian tumor measuring 65 mm×35 mm,without signs of invasive or metastatic *** explorative laparotomy,normal morphology of the internal reproductive organs was noted.A solid mobile lesion involved the entire *** was *** of the abdominal cavity revealed no signs of malignant ***,the appendiceal lesion corresponded to a completely resected low-grade mucinous appendiceal neoplasm(LAMN).CONCLUSION The appropriate treatment and team of specialists who should provide health care to patients with seemingly adnexal lesions depend on the nature(benign vs malignant)and origin(gynecological vs nongynecological)of the ***,gynecologists and other pelvic surgeons should be familiar with the imaging signs of LAMN whose clinical presentation is silent or *** assistance of a consultant specializing in intestinal tumors is important support that gynecological surgeons can receive during the operation

关键词： Adnexal mass Appendiceal neoplasm Diagnostic imaging Pelvic neoplasm Adnexal diseases Pelvic neoplasm Case report

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Colon stricture after hemolytic uremic syndrome

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World Journal of Pediatric Surgery 2021年第2期4卷 33-35页

作者： Maria Luis Sacras catarina Ladeira Sara Cordeiro pereira Rui Alves Pediatric Surgery Department Hospital Dona EstefaniaLisboaPortugal

Colonic stricture is a rare complication of hemolytic uremic syndrome(HUS).Risk factors include severe colitis,female gender and younger age.A clinical presentation of bowel obstruction in children,with previous known HUS,should prompt a contrast enema to rule out areas of *** treatments consist of laparotomy,resection of the affected bowel and primary anastomosis.

关键词： stricture hemolytic clinical

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Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

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Regenerative Biomaterials 2021年第1期8卷 73-82页

作者： Isabel pereira Jose Eduardo pereira Luis Maltez Alexandra Rodrigues catarina Rodrigues Manuela Oliveira Dina MSilva Ana Rita Caseiro Justina Prada Ana Colette Mauricio Jose Domingos Santos Miguel Gama CEB Centre of Biological EngineeingUniversity of MinhoCampus de GualtarBraga 4710-057Portugal CECAV Animal and Veterinary Research CentreUniversity of Trias-os-Montes e Alto DouroVila Real 5001-801Portugal Department of Veterinary Sciences University of Tras-os-Montes e Alto DouroVila Real 5001-801Portugal Bios ckin Molecular and Cell Therapies S.A.Laboratorio CriovidaTecMaiaRua Engenheiro Frederico Ulrich 2650Moreira da Maia 4470-605Portugal Departamento de Clinicas Veterinarias Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)Universidade do Porto(UP)Rua de Jorge Viterbo Ferreiran°228Porto 4050-313Portugal Centro de Estudos de Ciencia Animal(CECA) Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua D.Manuel IApartado 55142Porto 4051-401 Portugal Centro de Investigaqao Vasco da Gama(CIVG)/Escola Universitaria Vasco da Gama(EUVG) Avenida Jose R.Sousa Femandesn°197 LordemaoCoimbra 3020-210Portugal REQUIMTE/LAQV Departamento de Engenharia Metalurgica e MateriaisFaculdade de EngenhariaUniversidade do PortoRua Dr Roberto FriasPorto 4200-495Portugal

The development of injectable bone substitutes(IBS)have obtained great importance in the bone re-generation field,as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular *** this scenario,the association of injectable hydrogels and bone graft granules is emerging as a well-established ***,in situ forming hydrogels have arisen as a new IBS *** in situ forming and injectable dextrin-based hydrogel(HG)was developed,aiming to act as a carrier of granular bone substitutes and bioactive *** this work,the HG was associated to a granular bone substitute(Bonelike)and implanted in goat critical-sized calvarial defects(14mm)for 3,6 and *** results showed that HG improved the han-dling properties of the Bonelike granules and did not affect its osteoconductive features,neither impairing the bone regener ation *** multipotent mesenchymal stromal cells from the umbilical cord,extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the *** bioactive agents did not enhance the new bone formation significantly under the conditions tested,according to micro-computed tomography and histological analysis.

关键词： polysaccharide injec table hydrogel granular ceramics Bonelike' bone regen eration calvarial defect

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Fluid-dynamics evaluation in a conical spouted bed and characterization of foxtail millet seeds

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Particuology 2015年第6期13卷 75-81页

作者： Lidiane Diniz do.Nascimento Lorena Gomes Corumba Sandra Cristina dos Santos Rocha Osvaldir pereira Taranto Cristiane Maria Leal Costa Lenio Jose guerreiro de Faria Federal University of Pard Faculty of Chemical Engineering Laboratory of Drying and Particle Coating Augusto Correa Street w/n 66075-110 BelemPA Brazil University of Campinas School of Chemical Engineering. Albert Einstein Avenue 500 13083-852 Campinas $8o Paulo Brazil

Foxtail millet （Setaria italica） is one of the most valuable species in economic terms in the genus Setaria and plays an important role in human nutrition, animal feed, and agriculture. The present study described chemical, physical, and quality aspects of seeds of foxtail millet. Furthermore, the fluid-dynamic behavior of the seeds was evaluated in a conical spouted bed, which has advantages in terms of promoting the cyclic and regular movement of the seed particles. Dynamic parameters of spouting （minimum spouting veloc- ity, stable and peak pressure drop） were determined and compared with those obtained from empirical correlations available in the literature. The results obtained from physical characterization showed that the seeds can be classified as belonging to Group D of Geldart, having a non-rough surface, mean diameter of 1.75 mm, and sphericity of 0.74. Fluid-dynamics analysis showed that the seeds are suitable for processing in a spouted bed, which is in agreement with the results of particle physical characterization.

关键词： Foxtail millet Parameters of spouting Characterization Conical spouted bed

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Pramipexole, a dopamine D3/D2 receptor-preferring agonist, attenuates reserpine-induced fibromyalgia-like model in mice

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Neural Regeneration Research 2022年第2期17卷 450-458页

作者： Carlos pereira Martins Rodrigo Sebben Paes Gabriela Mantovani Baldasso Eduarda Gomes Ferrarini Rahisa Scussel Rubya pereira Zaccaron Ricardo Andrez Machado-de-Avila Paulo Cesar Lock Silveira Rafael Cypriano Dutra Laboratory of Autoimmunity and Immunopharmacology Department of Health SciencesCampus AraranguaUniversidade Federal de Santa CatarinaAraranguáSCBrazil Post-Graduate Program of Neuroscience Center of Biological SciencesUniversidade Federal de Santa CatarinaFlorianópolisSCBrazil Laboratory of Experimental Physiopathology Program of Postgraduate in Science of HealthUniversidade do Extremo Sul CatarinenseCriciumaSCBrazil

Fibromyalgia(FM) is a complex pathology described as persistent hyperalgesia including somatic and mood dysfunctions, depression and anxiety. Although the etiology of FM is still unknown, a significant decrease in biogenic amines is a common characteristic in its pathogenesis. Here, our main objective was to investigate the role of dopamine D3/D2 receptor during the reserpine-induced pain in mice. Our results showed that pramipexole(PPX) – a dopaminergic D3/D2 receptor agonist – inhibited mechanical allodynia and thermal sensitivity induced by reserpine. Relevantly, PPX treatment decreased immobility time and increased the number of grooming in the forced swimming test and splash test, respectively. Animals that received PPX remained longer in the open arms than the reserpine group using elevated plusmaze apparatus. The repeated PPX administration, given daily for 4 days, significantly blocked the mechanical and thermal allodynia during FM model, similarly to pregabalin, although it failed to affect the reserpine-induced thermal nociception. Reserpine administration induced significant downregulation of dopamine concentration in the central nervous system, and repeated treatment with PPX restored dopamine levels in the frontal cortex and spinal cord tissues. Moreover, PPX treatment inhibited oxidants production such as DCFH(2′,7′-dichlorodihydrofluorescein) and nitrite, also decreased oxidative damage(carbonyl), and upregulated the activity of superoxide dismutase in the spinal cord. Together, our findings demonstrated the ability of dopamine D3/D2 receptor-preferring agonist in reducing pain and mood dysfunction allied to FM in mice. All experimental protocols were approved by the Universidade Federal de Santa catarina(UFSC) Ethics Committee(approval No. 2572210218) on May 10, 2018.

关键词： dopamine dopaminergic system fibromyalgia hyperalgesia pain pramipexole reserpine

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Diet as an epigenetic factor in inflammatory bowel disease

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World Journal of Gastroenterology 2023年第41期29卷 5618-5629页

作者： Karina Marangoni Gilson Dorneles Daniella Miranda da Silva Letícia pereira Pinto Carina Rossoni Sabrina Alves Fernandes Egas Moniz School of Health and Science Caparica-AlmadaPortugalCaparica 2820-062Portugal National Institute of Sciences and Technology-Theranostics and Nanobiotechnology Federal University of Uberlandia-MGBrazilUberlândia 38400-902Brazil Corporate Social Responsibility Hospital Moinhos de VentoPorto Alegre 90035-004Brazil Postgraduate Program in Gastroenterology Universidade Federal do Rio Grande do SulPorto Alegre 91540-000Brazil Department of Nutrition Uniasselvi-Group VitruSanta Catarina 89082-262Brazil Postgraduate Program in Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90050-170Brazil Master in Physical Activity and Health Polytechnic Institute of BejaBeja 7800-000Portugal Degree in Nutrition Sciences Lusófona UniversityLisboa 1749-024Portugal

Inflammatory bowel disease(IBD)has as a main characteristic the exacerbation of the immune system against enterocytes,compromising the individual’s intestinal *** inflammatory cascade causes several nutritional deficiencies,which further compromise immunological functioning and,as a result,worsen the *** vicious cycle can be interrupted as the patient’s dietary pattern meets their needs according to their clinical condition,acting directly on the inflammatory process of IBD through the interaction of food,intestinal microbiota,and *** nutritional intervention for IBD has a crucial role in preventing and managing disease *** review addresses epigenetic modifications through dietary compounds as a mechanism for modulating the intestinal microbiota of patients with IBD.

关键词： Inflammatory bowel disease Epigenetics Nutrition Nutrigenetics

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