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世界竹藤通讯 2012年第3期10卷 48-48页

作者： Juan carlos Camargo Garcia 佩雷拉理工大学

瓜多竹是一种木本竹类，它代表着在哥伦比亚特别是哥伦比亚咖啡地区的一种重要的自然资源。在传统上，瓜多竹被农民用来制造房屋、家具、工艺品、附聚物（agglomerates）、胶合板和地板等产品。这些竹林几乎是这一地区现存在海拔900-20... 详细信息

瓜多竹是一种木本竹类，它代表着在哥伦比亚特别是哥伦比亚咖啡地区的一种重要的自然资源。在传统上，瓜多竹被农民用来制造房屋、家具、工艺品、附聚物（agglomerates）、胶合板和地板等产品。这些竹林几乎是这一地区现存在海拔900-2000m之间的唯一森林植被，因为大多数的森林在很久以前由于农业的原因已被砍伐。因此，目前该地区的天然林中只残存了以瓜多竹为主的片林。瓜多竹林高度分散和而且大多数片林不会大于5hm2。然而，这些森林是生物多样性重要的庇护所，在这些森林中已被查明的木本植物超过100种。此外，它们为约69种鸟类和5种哺乳动物（蝙蝠）提供了栖息地，履行了重要生态功能。此外，瓜多竹林也可以被用来作为对气候变化的适应和减缓具有推动性战略的指示物。本文基于这个意义，分析了瓜多竹林纳入REDD＋框架的可能性，以及与此相关的各种因素，并组成一个由4个模块确定的结构：即范围，参考标准，分布特征和融资水平。此后，不管这个项目是否实现，只要集中精力，这个结构的建立是有可能的。瓜多竹林固碳能力平均为109±54t／hm2，就像体制和政治的发展一样显著，它的碳汇能力可能有助于降低REDD＋项目实施的难度。在关于倡议REDD＋的监测和主流化战略作为区域内的一个优先举措等方面，相关部门和制度的规划过程必须要统一。

关键词：适应性减排生物多样性 REDD 生态系统服务主流化

Prevalence of G6PD deficiency and hemoglobinopathies on malaria-endemic Bioko Island,Equatorial Guinea:implications for public health strategies in the region

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Prevalence of G6PD deficiency and hemoglobinopathies on mala...

赤道几内亚比奥科岛G6PD缺乏症分子流行病学研究

广东省遗传学会第九届代表大会暨学术研讨会

作者： Min Lin Dong-De Xie Jiang-tao Chen Santiago-m Monte Nguba carlos Sala Ehapo Li-Ye Yang Xiao-Fen Zhan Juan Urbano Monsuy Eyi Rocio Apicante Matesa Maximo Miko Ondo Obono Hui Yang Hui-tian Yang Li-Ye Yang Department of Internal Medicine First Hospital Affiliated with the Medical College of Shantou University Central Laboratory Chaozhou Central Hospital Affiliated to Southern Medical University

Background to propose effective public health strategies for using antimalarial drugs and prevention and control of genetic disorders,reliable epidemiological information on local population is *** study aimed to assess the prevalence and health impact of glucose-6-phosphate dehydrogenase(G6PD) deficiency and hemoglobinopathies in the population on malaria-endemic Bioko Island,Equatorial *** Blood samples from 4,144 unrelated subjects were screened for G6 PD deficieny by fluorescence spot test,high-resolution melting assay and PCR-DNA sequencing from April 2012 to May *** addition,1,186 samples were randomly selected from the 4,144 subjects for detection of hemoglobin S(HbS;c.20 A>t),HbC(c.19 G>A),and a-thalassemia(a-thal) deletion by complete blood count,PCR-DNA sequencing and reverse dot blot genechip *** the prevalence of malaria and anemia was 12.6%(522/4,144) and 32.8%(389/1,186),***,8.7%people(359/4,144) were G6PD-deficient,9.0%(249/2,758) males and 7.9%(110/1,386)*** A" variants(c.202 G>A + c.376 A>G) were detected in 99.2%of G6PD-deficient individuals(356/359).Among the 1,186 subjects screened for hemoglobinopathies,201 were HbS heterozygotes,35 HbC heterozygotes,and 2 HbCS double heterozygotes;452 showed heterozygous-a3’7 kb deletion and 85 homozygous-a3’7 kb *** overall allele frequencies for these disorders were HbS,17.1%(n=203);HbC,3.1%(n=37);and a-thal,45.3%(n=622).Conclusions High G6 PD deficiency in this population indicated that diagnosis and management of G6 PD deficiency is crucial for current malaria control and *** should be tested for G6 PD deficiency before antimalarial *** newborn screening,prenatal screening and counseling for these genetic disorders,especially HbS,are needed in the region.

关键词： G6PD deficeny hemoglobinopathies Bioko Island

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分子诊断与治疗杂志 2014年第5期6卷 296-301页

作者：詹小芬陈江涛谢东德杨辉杨惠钿杨立业陆志为 Santiago-m Monte-Nguba Juan carlos Salas Ehapo Urbano Monsuy Eyi 林敏南方医科大学附属潮州市中心医院检验实验中心广东潮州521000 中国第25批援赤道几内亚医疗队广东广州510000 广东省惠州市中心医院检验中心广东惠州516001 中国第26批援赤道几内亚医疗队广东广州510000 潮州市公安局法医鉴定中心广东潮州521000 Malabo Regional Hospital Malabothe Republic of Equatorial Guinea

目的探讨非州西部赤道几内亚比奥科岛(Bioko Island)人群的葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症的发生率及基因型。方法在2012年1月至5月期间,用荧光斑点法对2 187名比奥科岛当地居民进行G6PD缺乏症筛查。采用高分辨熔解曲线(High-resoluti... 详细信息

目的探讨非州西部赤道几内亚比奥科岛(Bioko Island)人群的葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症的发生率及基因型。方法在2012年1月至5月期间,用荧光斑点法对2 187名比奥科岛当地居民进行G6PD缺乏症筛查。采用高分辨熔解曲线(High-resolution melting,HRM)分析G6PD缺乏的标本的c.202 G>A与c.376 A>G。对HRM筛选不出突变的G6PD酶学缺乏的样本,针对非洲的其他突变类型:c.542 A>t(rs5030872)、c.680 G>A(rs137852328)、c.968 t>C(rs76723693)进行PCR-DNA测序。结果赤道几内亚比奥科岛人群的G6PD缺乏症总发生率为8.54%(189/2 187),其中男性84例(9.04%,84/929),女性105例(8.34%,105/1 258),男女检出率比为1.08:1。在189例G6PD缺乏标本中共检出两种基因类型,其中包括G6PD A-变异体(c.376 A>G/c.202 G>A)186例(98.41%,186/189G6PD Betica(c.376 A>G/c.968t>C)3例(1.59%3/189)。结论赤道几内亚比奥科岛是G6PD缺乏症高发区,基因型比较单一。HRM技术可用于非洲地区G6PD缺乏症的临床诊断和流行病学研究。

关键词： G6PD缺乏症比奥科岛高分辨熔解曲线基因型 PCR-DNA测序

非运动症状负担、情绪和步态障碍是导致帕金森病非痴呆患者生活质量下降的最重要因素

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中华医学杂志 2020年第27期100卷 2091-2091页

作者：张煜(编译) Santos Garcia D de Deus Fonticoba t Suarez Castro E 上海交通大学医学院附属新华医院神经内科不详

帕金森病存在多种运动和非运动症状表现,其中非运动症状对于帕金森病患者生活质量的影响更为严重。改善帕金森病非运动症状,有助于提高患者的生活质量。既往帕金森病非运动症状研究多为单中心小样本研究,所涉及的非运动症状类型较为局限... 详细信息

帕金森病存在多种运动和非运动症状表现,其中非运动症状对于帕金森病患者生活质量的影响更为严重。改善帕金森病非运动症状,有助于提高患者的生活质量。既往帕金森病非运动症状研究多为单中心小样本研究,所涉及的非运动症状类型较为局限,无法全面反映非运动症状对帕金森病患者生活质量的影响。为此,该研究通过分析COPPADIS-2015研究队列来关注帕金森病患者非运动症状对其生活质量的影响。该队列是西班牙全国范围内为期5年的多中心综合观察性随访队列研究。

关键词：非运动症状帕金森病患者队列研究生活质量的影响患者生活质量综合观察步态障碍小样本研究

Single-cell transcriptomic atlas of mouse cochlear aging

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Protein & Cell 2023年第3期14卷 180-201页

作者： Guoqiang Sun Yandong Zheng Xiaolong Fu Weiqi Zhang Jie Ren Shuai Ma Shuhui Sun Xiaojuan He QiaoranWang zhejun ji Fang Cheng KaowenYan Ziyi Liu Juan carloszpisuaemonte Jing Qu Si Wang Renjie Chai Guang-Hui Liu State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Bioelectronics Jiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSchool of Life Sciences and TechnologySoutheast UniversityNanjing 211189China Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical University.Beijing100053China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Aging translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital MedicalUniversityBeijing100053China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cell and Regeneration CASBeijing 100101China China National Center for Bioinformation Beijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China the Fifth People's Hospital of Chongqing Chongqing 400062China Gene Expression Laboratory Salk Institute for Biological StudiesLa JollaCA 92037USA Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Shandong Provincial Hospital and School of Laboratory Animal Science Shandong First Medical UniversityJinan 250000China

Progressive functional deterioration in the cochlea is associated with age-related hearing loss(ARHL).However,the cellular and molecular basis underlying cochlear aging remains largely ***,we established a dynamic single-cell transcriptomic landscape of mouse cochlear aging,in which we characterized aging-associated transcriptomic changes in 27 different cochlear cell types across five different time ***,our analysis pinpoints loss of proteostasis and elevated apoptosis as the hallmark features of cochlear aging,highlights unexpected age-related transcriptional fluctuations in intermediate ceils localized in the stria vascularis(SV)and demonstrates that upregulation of endoplasmic reticulum(ER)chaperon protein HSP90AA1 mitigates ER stress-induced damages associated with *** work suggests that targeting unfolded protein response pathways may help alleviate aging-related sVatrophyand hencedelay theprogressionofARHL.

关键词： single-cell transcriptomic atlas mouse cochlea aging

“Magic” of our gastric cancer results on perioperative chemotherapy

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World Journal of Gastrointestinal Pathophysiology 2016年第3期7卷 283-287页

作者： carlos León-Espinoza Fernando López-Mozos Roberto Marti-Obiol Marina Garces-Albir Joaquin Ortega-Serrano Department of Digestive and General Surgery Hospital Clínico Universitario de Valencia

AIM: to determine reproducibility of perioperative chemotherapy for gastric cancer(GC) on our settings by identifying patient's overall survival and comparing them to larger ***: Retrospective analysis of our series,where we present our eleven-year's experience on GC managed according to perioperative approach of three preoperative chemotherapy cycles followed by surgery and finally three postoperative chemotherapy *** scheme used was Xelox(Oxaliplatin and Capecitabine).Epidemiologic parameters as well as surgical variables were analysed,presented,and compared to other series with similar *** was estimated by Kaplan Meier/log rank method and also compared to these ***: Mean age was 65 years *** survival in our series was 37.7%,similar to other groups using perioperative *** was 4% and morbidity 30%,which are also similar to those *** curves were compared to larger studies,finding similarities on *** survival analysis between chemotherapy responders and non-responders didn't reach statically significant ***: Perioperative chemotherapic scheme can be reproduced on our setting with good results and without increasing morbidity or mortality.

关键词： Stomach neoplasms Perioperative period Chemotherapy

Species diversity of Basidiomycota

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Fungal Diversity 2022年第3期114卷 281-325页

作者： Mao-Qiang He Rui-Lin Zhao Dong-Mei Liu teodor t.Denchev Dominik Begerow Andrey Yurkov Martin Kemler Ana M.Millanes Mats Wedin A.R.Mctaggart Roger G.Shivas Bart Buyck Jie Chen Alfredo Vizzini Viktor Papp Ivan V.Zmitrovich Naveed Davoodian Kevin D.Hyde State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101People’s Republic of China College of Life Sciences University of Chinese Academy of SciencesHuairou DistrictBeijing 100408People’s Republic of China Institute of Ecology Chinese Reserach Academy of Environmental SciencesBeijing 100012People’s Republic of China Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences2 Gagarin St.1113 SofiaBulgaria Evolution der Pflanzen und Pilze Ruhr-Universität BochumND 03Universitätsstr.15044801 BochumGermany Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Inhoffenstraße 7B38124 BraunschweigGermany Departamento de Biología y Geología Física y Química InorgánicaUniversidad Rey Juan Carlos28933 MóstolesSpain Department of Botany Swedish Museum of Natural HistoryP.O.Box 5000710405 StockholmSweden Queensland Alliance for Agriculture and Food Innovation The University of QueenslandBrisbane 4001Australia Centre for Crop Health University of Southern QueenslandToowoomba 4350Australia Institut pour Systematique EcologieBiodiversite(ISYEBUMR 7205)Sorbonne universitéMuséum National d’Histoire Naturelle CP 39CNRSDépartement Origines et Evolution12 Rue Buffon75005 ParisFrance Facultad de Ciencias Biológicas y Agropecuarias PeñuelaUniversidad VeracruzanaAmatlán de los Reyes94945 VeracruzMéxico Dipartimento di Scienze della Vita e Biologia dei Sistemi Universitàdi TorinoViale P.A.Mattioli 2510125 TorinoItaly Department of Botany Hungarian University of Agriculture and Life Sciences1518 BudapestHungary Komarov Botanical Institute Russian Academy of SciencesProfessor Popov st.2Saint PetersburgRussia 197376 Royal Botanic Gardens Victoria Birdwood AvenueMelbourneVIC 3004Australia Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of Science

Fungi are eukaryotes that play essential roles in *** fungi,Basidiomycota is one of the major phyla with more than 40,000 described *** review species diversity of Basidiomycota from five groups with different lifestyles or habitats:saprobic in grass/forest litter,wood-decaying,yeast-like,ectomycorrhizal,and plant *** studies of Agaricus,Cantharellus,Ganoderma,Gyroporus,Russula,tricholoma,and groups of lichenicolous yeast-like fungi,rust fungi,and smut fungi are used to determine trends in discovery of *** each case study,the number of new species published during 2009–2020 is analysed to determine the rate of *** rates differ between taxa and reflect different states of progress for species discovery in different *** results showed that lichenicolous yeast-like taxa had the highest publication rate for new species in the past two decades,and it is likely this trend will continue in the next *** species discovery rate of plant parasitic basidiomycetes was low in the past ten years,and remained constant in the past 50 *** also found that the establishment of comprehensive and robust taxonomic systems based on a joint global initiative by mycologists could promote and standardize the recognition of *** estimated that more than 54,000 species of Basidiomycota will be discovered by 2030,and estimate a total of 1.4–4.2 million species of Basidiomycota *** numbers illustrate a huge gap between the described and yet unknown diversity in Basidiomycota.

关键词： Biodiversity Fungi Species number taxonomy

Monitoring by 1H Nuclear Magnetic Resonance of the Glycerin Purification by Vacuum Distillation

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材料科学与工程（中英文A版） 2013年第9期3卷 628-634页

作者： Ignacio Contreras Andrade Fabio Emiro Sierra Vargas Jonathan Fabian Sierra Cantor Eliseo Avella Moreno carlos Alberto Guerrero Fajardo Faculty of Chemical Biological Science Universidad Autonoma de Sinaloa 80040 Culiacan Mexico Department of Chemistry Universidad Nacional de Colombia Bogota 11001 Colombia

Given the growth in biodiesel production and other biofuels, it involves large volumes of byproducts which aren"t suitable for commercial use. We studied the glycerol purification by vacuum distillation of the glycerol rich phase in the mixture reaction at the end of the biodiesel production process. Since glycerin is one of those byproducts, useful and plentiful, therefore the options to purify this one and achieve its better trading benefit are interesting. the process was monitored by 1 H NMR and showed that by vacuum fractional distillation of the glycerin rich phase of the biodiesel production it was possible to obtain pure glycerin between 97.4% and 98.9% on dry basis with 49.0% yield. Vacuum distillation without fractionation was inconvenient because it generates thermal degradation of the material and causes the formation of foam which induces low yield of glycerin and hinders the operation progress.

关键词：真空蒸馏甘油核磁共振监测提纯生物柴油生产过程混合物反应

Detection of Babesia bovis and Babesia bigemina in Water Buffaloes (Bubalus bubalis) in Endemic Areas of São Paulo State, Brazil

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Open Journal of Veterinary Medicine 2016年第5期6卷 75-84页

作者： t. A. Néo R. Giglioti D. Obregón t. B. Bilhassi H. N. Oliveira R. Z. Machado F. de F. Aníbal L. G. Brito W. Malagó Jr. F. A. Bressani M. C. S. Oliveira Department of Morphology and Pathology Universidade Federal de S&atildeo Carlos S&atildeo Carlos Brazil Department of Animal Science Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Jaboticabal Brazil Agrarian University of Havana San José de las Lajas Cuba Department of Veterinary Pathology Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Jaboticabal Brazil Embrapa Rondônia Porto Velho Brazil Embrapa Pecuária Sudeste S&atildeo Carlos Brazil

Babesiosis is a tick-transmitted disease that causes severe economic losses to the cattle industry in Brazil. Water buffaloes (Bubalus bubalis) are often carriers of Babesia spp., but there are no studies that provide an accurate estimation of this infection in animals raised in regions of endemic stability. this study was conducted to investigate Babesia bovis and B. bigemina infections in 108 water buffaloes (50 calves and 58 adult females) located in areas of São Paulo state, where the animals were continuously exposed to Rhipicephalus microplus ticks. B. bovis and B. bigemina infections were screened by microscopic examination of blood smears, nested PCR (nPCR) and quantitative real-time PCR (qPCR), which were also used to estimate the number of copies (NC) of the cytochrome b (mt-cytB) gene in the blood samples. B. bigemina was found in blood smears of three calves from Alambari herd (all with less than 0.1% parasitemia). Molecular techniques were more sensitive than blood smears to diagnose piroplasms in water buffaloes: 20.37% and 100.00% for B. bovis-infected animals and 59.26% and 100.00% for B. bigemina-infected animals, respectively for nPCR and qPCR. the NC of mt-cytB gene of B. bovis and B. bigemina in blood samples revealed significant effects (p p ≤ 0.05) for B. bigemina (2.80 ± 0.06) than for B. bovis (2.61 ± 0.05). Within each herd-age, differences between the species’ NC values were found only in Alambari calves, which showed significantly higher (p ≤ 0.05) NC of B. bigemina (3.48 ± 0.13). the calves and cows from Ibaté showed the lowest NC of B. bigemina (2.29 ± 0.13 and 2.63 ± 0.14) and B. bovis (2.54 ± 0.11 and 2.37 ± 0.12), respectively. these data suggest a high prevalence of B. bovis and B. bigemina infection in the buffalo population in endemic areas of São Paulo state.

关键词： Bubalus bubalis Babesiosis Diagnosis nPCR qPCR