Single-cell transcriptomic atlas of mouse cochlear aging

作     者：Guoqiang Sun Yandong Zheng Xiaolong Fu Weiqi Zhang Jie Ren Shuai Ma Shuhui Sun Xiaojuan He QiaoranWang zhejun ji Fang Cheng KaowenYan Ziyi Liu Juan Carloszpisuaemonte Jing Qu Si Wang Renjie Chai Guang-Hui Liu Guoqiang Sun;Yandong Zheng;Xiaolong Fu;Weiqi Zhang;Jie Ren;Shuai Ma;Shuhui Sun;Xiaojuan He;Qiaoran Wang;Zhejun Ji;Fang Cheng;Kaowen Yan;Ziyi Liu;Juan Carlos Izpisua Belmonte;Jing Qu;Si Wang;Renjie Chai;Guang-Hui Liu

作者机构：State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of BioelectronicsJiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSchool of Life Sciences and TechnologySoutheast UniversityNanjing 211189China Advanced Innovation Center for Human Brain ProtectionNational Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical University.Beijing100053China CAS Key Laboratory of Genomic and Precision MedicineBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China National Laboratory of BiomacromoleculesCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Aging Translational Medicine CenterInternational Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital MedicalUniversityBeijing100053China University of Chinese Academy of SciencesBeijing 100049China Institute for Stem Cell and RegenerationCASBeijing 100101China China National Center for BioinformationBeijing 100101China Beijing Institute for Stem Cell and Regenerative MedicineBeijing 100101China The Fifth People's Hospital of ChongqingChongqing 400062China Gene Expression LaboratorySalk Institute for Biological StudiesLa JollaCA 92037USA Co-Innovation Center of NeuroregenerationNantong UniversityNantong 226001China Shandong Provincial Hospital and School of Laboratory Animal ScienceShandong First Medical UniversityJinan 250000China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2023年第14卷第3期

页      面：180-201页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(No.2020YFA0804000) the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16000000) the National Natural Science Foundation of China(Nos.82192863,81921006,92149301,92168201,91949209,92049304,82125011,82122024,82071588,92049116,32121001,32000500,82030029,81970882,31900523,82271600,32200610,and 81861168034) the National Key Research and Development Program of China(Nos.2018YFC2000100,2021ZD0202400,2020YFA0112200,2018YFA0107203,2021YFF1201005,and2019YFA0110100) the Program of the Beijing Natural Science Foundation(No.Z190019) CAS Project for Young Scientists in Basic Research(No.YSBR-076 and YSBR-012) the Key Research Program of the Chinese Academy of Sciences(No.KFZD-SW-221) K.C.Wong Education Foundation(Nos.GJTD-2019-06 and GJTD-2019-08) Youth Innovation Promotion Association of CAS(Nos.2021078,2022083,and E1CAZW0401) Young Elite Scientists Sponsorship Program by CAST(Nos.YESS20200012 and YESS20210002) the State Key Laboratory of Stem Cell and Reproductive Biology,the State Key Laboratory of Membrane Biology,the Tencent Foundation(No.2021-1045) the Informatization Plan of Chinese Academy of Sciences(Nos.CAS-WX2021SF-0301 and CASWX2022SDC-XK14) the Pilot Project for Public Welfare Development and Reform of Beijing-affliated Medical Research Institutes(No.11000022T000000461062) Natural Science Foundation from Jiangsu Province(No.BE2019711),Shenzhen Fundamental Research Program(No.JCYJ20190814093401920) Open Research Fund of State Key Laboratory of Genetic Engineering,Fudan University(No.SKLGE-2109). 

主　　题：single-cell transcriptomic atlas mouse cochlea aging 

摘      要：Progressive functional deterioration in the cochlea is associated with age-related hearing loss(ARHL).However,the cellular and molecular basis underlying cochlear aging remains largely unknown.Here,we established a dynamic single-cell transcriptomic landscape of mouse cochlear aging,in which we characterized aging-associated transcriptomic changes in 27 different cochlear cell types across five different time points.Overall,our analysis pinpoints loss of proteostasis and elevated apoptosis as the hallmark features of cochlear aging,highlights unexpected age-related transcriptional fluctuations in intermediate ceils localized in the stria vascularis(SV)and demonstrates that upregulation of endoplasmic reticulum(ER)chaperon protein HSP90AA1 mitigates ER stress-induced damages associated with aging.Our work suggests that targeting unfolded protein response pathways may help alleviate aging-related sVatrophyand hencedelay theprogressionofARHL.