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science China(Life sciences) 2004年第3期47卷 224-228页

作者： CHENG Lingpeng1, CHEN Senxiong1, Jenifer m. Brannan2, Joanita Jakana3, ZHANG Qinfen1, Z. H. Zhou2 & ZHANG Jingqiang1 1. State Key Laboratory for Bio-control, Zhongshan University, Guangzhou 510275, China 2. Department of Pathology, medicine University of Texas-Huston medical School, Huston, tx77030, USA 3. National center for macromolecular Imaging, baylor college of medicine, Houston, tx77, USA State Key Laboratory for Bio-control Zhongshan University Guangzhou 510275 China Department of Pathology Medicine University of Texas-Huston Medical School Huston TX77030 USA National center for macromolecular Imaging Baylor College of Medicine Houston TX77 USA

The three-dimensional structure of capsid of Aedes albopictus C6/36 densovirus was determined to 14- resolution by electron cryomicroscopy and computer reconstruction. The triangulation number of the capsid is 1. There are 12 holes in each triangular face and a spike on each 5-fold vertex. The validity of the capsid and nucleic acid densities in the reconstructions was discussed.

关键词： electron cryomicroscopy, three-dimensional reconstruction, Aedes albopictus densovirus.

3-Dimensional Kinematic Comparison of Arm movements between an Individual with NGLY1 Deficiency and a Neurotypical Individual

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Case Reports in Clinical medicine 2024年第4期13卷 122-146页

作者： Charles S. Layne Christopher A. malaya Brock Futrell Dacia martinez Diaz Christian Alfaro Hannah E. Gustafson Subhalakshmi Chandrasekaran Rhea m. Phatak Bernhard Suter Department of health and Human Performance University of Houston Houston TX USA center for Neuromotor and Biomechanics Research University of Houston Houston TX USA center for Neuro Engineering and Cognitive science University of Houston Houston TX USA Department of Computer science and Engineering Texas A&M University College Station TX USA Blue Bird Circle Rett center Texas Children&#8217s Hospital Houston TX USA baylor college of medicine Houston TX USA

NGLY1 Deficiency is an ultra-rare autosomal recessively inherited disorder. Characteristic symptoms include among others, developmental delays, movement disorders, liver function abnormalities, seizures, and problems with tear formation. movements are hyperkinetic and may include dysmetric, choreo-athetoid, myoclonic and dystonic movement elements. To date, there have been no quantitative reports describing arm movements of individuals with NGLY1 Deficiency. This report provides quantitative information about a series of arm movements performed by an individual with NGLY1 Deficiency and an aged-matched neurotypical participant. Three categories of arm movements were tested: 1) open ended reaches without specific end point targets;2) goal-directed reaches that included grasping an object;3) picking up small objects from a table placed in front of the participants. Arm movement kinematics were obtained with a camera-based motion analysis system and “initiation” and “maintenance” phases were identified for each movement. The combination of the two phases was labeled as a “complete” movement. Three-dimensional analysis techniques were used to quantify the movements and included hand trajectory pathlength, joint motion area, as well as hand trajectory and joint jerk cost. These techniques were required to fully characterize the movements because the NGLY1 individual was unable to perform movements only in the primary plane of progression instead producing motion across all three planes of movement. The individual with NGLY1 Deficiency was unable to pick up objects from a table or effectively complete movements requiring crossing the midline. The successfully completed movements were analyzed using the above techniques and the results of the two participants were compared statistically. Almost all comparisons revealed significant differences between the two participants, with a notable exception of the 3D initiation area as a percentage of the complete movement. The stati

关键词： NGLY1 Deficiency Developmental Disorders Kinematics 3 Dimensional Analyses

deCS:A Tool for Systematic Cell Type Annotations of Single-cell RNA Sequencing Data among Human Tissues

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Genomics, Proteomics & Bioinformatics 2023年第2期21卷 370-384页

作者： Guangsheng Pei Fangfang Yan Lukas m.Simon Yulin Dai Peilin Jia Zhongming Zhao center for Precision health School of Biomedical InformaticsThe University of Texas Health Science Center at HoustonHoustonTX 77030USA Therapeutic Innovation center Baylor College of MedicineHoustonTX 77030USA Human Genetics center School of Public HealthThe University of Texas Health Science Center at HoustonHoustonTX 77030USA mD Anderson Cancer center UThealth Graduate School of Biomedical sciences HoustonTX 77030USA Department of Biomedical Informatics Vanderbilt University Medical CenterNashvilleTN 37203USA CAS Key Laboratory of Genomic and Precision medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China

Single-cell RNA sequencing(scRNA-seq)is revolutionizing the study of complex and dynamic cellular ***,cell type annotation remains a main challenge as it largely relies on a priori knowledge and manual curation,which is cumbersome and *** increasing number of scRNA-seq datasets,as well as numerous published genetic studies,has motivated us to build a comprehensive human cell type reference ***,we present decoding Cell type Specificity(deCS),an automatic cell type annotation method augmented by a comprehensive collection of human cell type expression profiles and marker *** used deCS to annotate scRNAseq data from various tissue types and systematically evaluated the annotation accuracy under different conditions,including reference panels,sequencing depth,and feature selection *** results demonstrate that expanding the references is critical for improving annotation *** to many existing state-of-the-art annotation tools,deCS significantly reduced computation time and increased *** can be integrated into the standard scRNA-seq analytical pipeline to enhance cell type ***,we demonstrated the broad utility of deCS to identify trait-cell type associations in 51 human complex traits,providing deep insights into the cellular mechanisms underlying disease pathogenesis.

关键词： Cell type-specific enrichment analysis scRNA-seq Cell type annotation Trait-cell type association Cell typemarkergene

CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice

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Asian Journal of Andrology 2022年第3期24卷 266-272页

作者： Yuki Oyama Haruhiko miyata Keisuke Shimada Yoshitaka Fujihara Keizo Tokuhiro Thomas X Garcia martin m matzuk masahito Ikawa Graduate School of Pharmaceutical sciences Osaka UniversitySuitaOsaka 565-0871Japan Department of Experimental Genome Research Research Institute for Microbial DiseasesOsaka UniversitySuitaOsaka 565-0871Japan Department of Bioscience and Genetics National Cerebral and Cardiovascular CenterSuitaOsaka 564-8565Japan Department of Genome Editing Institute of Biomedical ScienceKansai Medical UniversityHirakataOsaka 573-1191Japan center for Drug Discovery Baylor College of MedicineHoustonTX 77030USA Department of Pathology and Immunology Baylor College of MedicineHoustonTX 77030USA Department of Biology and Biotechnology University of Houston-Clear LakeHoustonTX 77058USA The Institute of medical science The University of TokyoMinato-kuTokyo 108-8639Japan

Gene expression analyses suggest that more than 1000–2000 genes are expressed predominantly in mouse and human *** functional analyses of hundreds of these genes have been performed,there are still many testis-enriched genes whose functions remain *** gene function using knockout(KO)mice is a powerful tool to discern if the gene of interest is essential for sperm formation,function,and male fertility in *** this study,we generated KO mice for 12 testis-enriched genes,1700057G04Rik,4921539E11Rik,4930558C23Rik,Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tmem210,and Tomm20l,using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)*** designed two gRNAs for each gene to excise almost all the protein-coding regions to ensure that the deletions in these genes result in a null *** tests of KO mice reveal that these 12 genes are not essential for male fertility,at least when individually ablated,and not together with other potentially compensatory paralogous *** results could prevent other laboratories from expending duplicative effort generating KO mice,for which no apparent phenotype exists.

关键词： CRISPR/Cas9 knockout mice male infertility spermatozoa testis

Fragile X messenger Ribonucleoprotein 1(FmR1), a novel inhibitor of osteoblast/osteocyte differentiation, regulates bone formation, mass, and strength in young and aged male and female mice

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Bone Research 2023年第2期11卷 384-397页

作者： Padmini Deosthale Julián Balanta-melo Amy Creecy Chongshan Liu Alejandro marcial Laura morales Julita Cridlin Sylvia Robertson Chiebuka Okpara David J.Sanchez mahdi Ayoubi Joaquín N.Lugo Christopher J.Hernandez Joseph m.Wallace Lilian I.Plotkin Department of Anatomy Cell Biology&PhysiologyIndiana University School of MedicineIndianapolisIN46202USA Roudebush Veterans Administration medical center IndianapolisIN46202USA Indiana center for musculoskeletal health IndianapolisIN46202USA Universidad del Valle School of dentistry Cali 760043Colombia Department of Biomedical Engineering Indiana University-Purdue University Indianapolis46202 IndianapolisIN46202USA Sibley School of mechanical and Aerospace Engineering Cornell UniversityIthacaNY 14853USA Department of materials science and Engineering Cornell UniversityIthacaNY14853USA Department of Psychology and Neuroscience Baylor UniversityWacoTX76798USA

Fragile X messenger Ribonucleoprotein 1(FmR1)gene mutations lead to fragile X syndrome,cognitive disorders,and,in some individuals,scoliosis and craniofacial ***-month-old(mo)male mice with deletion of the FmR1 gene exhibit a mild increase in cortical and cancellous femoral bone ***,consequences of absence of FmR1 in bone of young/aged male/female mice and the cellular basis of the skeletal phenotype remain *** found that absence of FmR1 results in improved bone properties with higher bone mineral density in both sexes and in 2-and 9-mo *** cancellous bone mass is higher only in females,whereas,cortical bone mass is higher in 2-and 9-mo males,but higher in 2-and lower in 9-mo female FmR1-knockout ***,male bones show higher biomechanical properties at 2mo,and females at both *** of FmR1 increases osteoblast/mineralization/bone formation and osteocyte dendricity/gene expression in vivo/ex vivo/in vitro,without affecting osteoclasts in vivo/ex ***,FmR1 is a novel osteoblast/osteocyte differentiation inhibitor,and its absence leads to age-,site-and sex-dependent higher bone mass/strength.

关键词： female messenger agile

Analytical strategies for studying stem cell metabolism

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Frontiers in Biology 2015年第2期10卷 141-153页

作者： James m. Arnold William T. Choi Arun Sreekumar mirjana maletic-Savatic Department of molecular and Cell Biology Baylor College of Medicine Houston TX 77030 USA Program in Developmental Biology and medical Scientist Training Program Baylor College of Medicine Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital Houston TX 77030 USA Departments of Pediatrics-Neurology and Neuroscience and Program in Structural and Computational Biology and Molecular Biophysics Baylor College of Medicine Houston TX 77030 USA

Owing to their capacity for self-renewal and pluripotency, stem cells possess untold potential for revolutionizing the field of regenerative medicine through the development of novel therapeutic strategies for treating cancer, diabetes, cardiovascular and neurodegenerative diseases. Central to developing these strategies is improving our understanding of biological mechanisms responsible for governing stem cell fate and self-renewal. Increasing attention is being given to the significance of metabolism, through the production of energy and generation of small molecules, as a critical regulator of stem cell functioning. Rapid advances in the field of metabolomics now allow for in-depth profiling of stem cells both in vitro and in vivo, providing a systems perspective on key metabolic and molecular pathways which influence stem cell biology. Understanding the analytical platforms and techniques that are currently used to study stem cell metabolomics, as well as how new insights can be derived from this knowledge, will accelerate new research in the field and improve future efforts to expand our understanding of the interplay between metabolism and stem cell biology.

关键词： stem cell metabolism NmR mass spectrometry mRS flux analysis

CLONING AND ORGANIZATION OF BOVINE mETALLOTHIONEIN GENES

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Chinese science Bulletin 1989年第11期34卷 953-956页

作者：沈孝宙杨卫民彭红 m.G.PETERSON J.F.B.mERCER SAVIO L.C.WOO Laboratories of Reproductive Biology and Department of Endocrinology Institute of Zoology Academia Sinica Beijing Birth Defects Research Institute Royal Children's Hospital Parkville Australia Department of Cell Biology Baylor College of Medicine Texas Medical Center Houston TX 77030 USA

I. INTRODUCTIONmetallothioneins(mT) are ubiquitously distributed in animal and plant kingdoms. They are cysteine-rich proteins that specifically bind heavy metal ions, such as cadmium, zinc, mercury and copper. The high expression of the mT gene could be induced by heavy metals in vitro and in vivo. The first transgenic mice obtained by Palmiter et al. in 1982 were introduced with an exogenous growth hormnoe gene

关键词： bovine metallothionein gene gene organization genomic cloning.

Association of latitude and altitude with adverse outcomes in patients with COVID-19: The VIRUS registry

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World Journal of Critical Care medicine 2022年第2期11卷 102-111页

作者： Aysun Tekin Shahraz Qamar Romil Singh Vikas Bansal mayank Sharma Allison m Lemahieu Andrew C Hanson Phillip J Schulte marija Bogojevic Neha Deo Simon Zec Diana J Valencia morales Katherine A Belden Smith F Heavner margit Kaufman Sreekanth Cheruku Valerie C Danesh Valerie m Banner-Goodspeed Catherine A St Hill Amy B Christie Syed A Khan Lynn Retford Karen Boman Vishakha KKumar John C O'Horo Juan Pablo Domecq Allan J Walkey Ognjen Gajic Rahul Kashyap Salim Surani TheSociety of Critical Care medicine(SCCm)Discovery Viral Infection and Respiratory Illness Universal Study(VIRUS):COVID-Registry Investigator Group Department of Anesthesiology Mayo ClinicRochesterMN 55905United States Post-baccalaureate Research Education Program Mayo Clinic College of Medicine and ScienceRochesterMN 55905United States Division of Pulmonary and Critical Care medicine Department of Internal MedicineMayo ClinicRochesterMN 55905United States Division of Clinical Trials and Biostatistics Department of Quantitative Health SciencesMayo ClinicRochesterMN 55905United States Alix School of medicine Mayo ClinicRochesterMN 55905United States Division of Infectious Diseases Thomas Jefferson University HospitalPhiladelphiaPA 19107United States Prisma health GreenvilleSC 29605United States Englewood health EnglewoodNJ 07631United States Divisions of Cardiothoracic Anesthesiology and Critical Care medicine Department of Anesthesiology and Pain ManagementUT Southwestern Medical CenterDallasTX 75390United States center for Applied health Research Baylor Scott and White HealthDallasTX 75246United States Department of Anesthesiology Critical Care and Pain MedicineBeth Israel Deaconess Medical CenterBostonMA 02215United States Allina health MinneapolisMN 55407United States Department of Critical Care Atrium Health NavicentMaconGA 31201United States Division of Critical Care medicine Mayo Clinic Health SystemMankatoMN 56001United States Society of Critical Care medicine Mount ProspectIL 60056United States Division of Infectious Diseases Mayo ClinicRochesterMN 55905United States Division of Nephrology and Hypertension Department of Internal MedicineMayo ClinicRochesterMN 55905United States Pulmonary center Division of PulmonaryAllergyCritical Care and Sleep MedicineDepartment of MedicineEvans Center of Implementation and Improvement SciencesBoston University School of MedicineBostonMA 02118United States Department of Pulmonary and Critical Care medicine Texas A&M UniversityBryanTX 77807United States 不详

BACKGROUND The coronavirus disease 2019(COVID-19)course may be affected by environmental *** studies previously suggested a link between climatological factors and COVID-19 fatality ***,individual-level impact of these factors has not been thoroughly evaluated *** To study the association of climatological factors related to patient location with unfavorable outcomes in *** In this observational analysis of the Society of Critical Care medicine Discovery Viral Infection and Respiratory Illness Universal Study:COVID-19 Registry cohort,the latitudes and altitudes of hospitals were examined as a covariate for mortality within 28 d of admission and the length of hospital *** for baseline parameters and admission date,multivariable regression modeling was *** estimating equations were used to fit the *** Twenty-two thousand one hundred eight patients from over 20 countries were *** median age was 62(interquartile range:49-74)years,and 54%of the included patients were *** median age increased with increasing latitude as well as the frequency of ***,the percentage of comorbidities was lower in elevated *** within 28 d of hospital admission was found to be 25%.The median hospital-free days among all included patients was 20 *** the significant linear relationship between mortality and hospital-free days(adjusted odds ratio(aOR)=1.39(1.04,1.86),P=0.025 for mortality within 28 d of admission;aOR=-1.47(-2.60,-0.33),P=0.011 for hospital-free days),suggesting that adverse patient outcomes were more common in locations further away from the Equator;the results were no longer significant when adjusted for baseline differences(aOR=1.32(1.00,1.74),P=0.051 for 28-day mortality;aOR=-1.07(-2.13,-0.01),P=0.050 for hospital-free days).When we looked at the altitude’s effect,we discovered that it demonstrated a non-linear association with mortality wi

关键词： 28 d mortality Altitude COVID-19 Hospital-free days Latitude Outcomes

Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization

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Protein & Cell 2021年第10期12卷 810-817页

作者： Yongcun Qu Qi Chen Shanshan Guo Chiyuan ma Yonggang Lu Junchao Shi Shichao Liu Tong Zhou Taichi Noda Jingjing Qian Liwen Zhang Xili Zhu Xiaohua Lei Yujing Cao Wei Li Wei Li Nicolas Plachta martin m.matzuk masahito Ikawa Enkui Duan Ying Zhang Hongmei Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of sciences Beijing 100049China Division of Biomedical sciences School of MedicineUniversity of CaliforniaRiversideCA 92521USA Research Institute for microbial Diseases Osaka UniversitySuitaOsakaJapan Department of Physiology and Cell Biology Reno School of MedicineUniversity of NevadaRenoNVUSA Institute of molecular and Cell Biology A*STARSingaporeSin gapore center for Drug Discovery Department of Pathology and ImmunologyBaylor College of MedicineHoustonTX 77030USA Division of Biology and Biological Engineering California Institute of TechnologyPasadenaCAUSA Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China The Key Laboratory of Cell Proliferation and Regulation Biology Ministry of EducationInstitute of Cell BiologyBeijing Normal UniversityBeijing 100875China

Dear Editor,Sperm cooperation has been observed in multiple species(Pizzari and Foster,2008),yet its existenee and benefit for reproductive success in mammals remains ***,combining tissue-clearing with deep three-dimensional(3D)imagi ng,we dem on strate that postcopulatory mouse sperm congregate into unidirectional sperm cooperative clusters at the utero-tubal junction(UTJ),a key physical barrier for passage into the oviduct.

关键词： passage media Foster