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Transcription factor CsESE3 positively modulates both jasmonic acid and wax biosynthesis in citrus

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aBIOTECH 2022年第4期3卷 250-266页

作者： Haoliang Wan Haiji Qiu Zhuoran Li Xiaoliang Zhang Jingyu Zhang Deyuan Jiang alisdair rfernie Yi Lyu Yunjiang Cheng Weiwei Wen Key Laboratory of Horticultural Plant Biology(MOE) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityWuhan430070China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518000China Max-Planck-Institute of Molecular Plant Physiology Am Muehlenberg 114476PotsdamGermany Key Laboratory for Space Bioscience and Biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an710072China

PLIP lipases can initiate jasmonic acid(JA)biosynthesis.However,little is known about the transcriptional regulation of this process.In this study,an ERF transcription factor(CsESE3)was found to be co-expressed with all necessary genes for JA biosynthesis and several key genes for wax biosynthesis in transcriptomes of‘Newhall’navel orange.CsESE3 shows partial sequence similarity to the well-known wax regulator SHINEs(SHNs),but lacks a complete MM protein domain.Ectopic overexpression of CsESE3 in tomato(OE)resulted in reduction of fruit surface brightness and dwarf phenotype compared to the wild type.The OE tomato lines also showed significant increases in the content of wax and JA and the expression of key genes related to their biosynthesis.Overexpression of CsESE3 in citrus callus and fruit enhanced the JA content and the expression of JA biosynthetic genes.Furthermore,CsESE3 could bind to and activate the promoters of two phospholipases from the PLIP gene family to initiate JA biosynthesis.Overall,this study indicated that CsESE3 could mediate JA biosynthesis by activating PLIP genes and positively modulate wax biosynthesis.The findings provide important insights into the coordinated control of two defense strategies of plants represented by wax and JA biosynthesis.

关键词： Citrus Cuticular wax Jasmonic acid SHINE transcription factor Lipase

Elucidation of the melitidin biosynthesis pathway in pummelo

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Journal of Integrative Plant Biology 2023年第11期65卷 2505-2518页

作者： Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou Ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu alisdair rfernie Jie Luo Sanya Nanfan Research Institute of Hainan University Hainan UniversitySanya 572025China National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory College of Life Science and TechnologyCollege of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Horticultural Plant Biology of Ministry of Education Huazhong Agricultural UniversityWuhan 430070China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria Yazhouwan National Laboratory Sanya 572025China

Specialized plant metabolism is a rich resource of compounds for drug discovery.The acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully characterized.Here,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2Rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of melitidin.Moreover,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug candidate.Co-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant system.This study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites.

关键词： bioactive metabolites biosynthetic pathway flavonoids melitidin natural variation

Natural variations of OsAUX5,a target gene of OsWRKY78,control the neutral essential amino acid content in rice grains

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Molecular Plant 2023年第2期16卷 322-336页

作者： Yuheng Shi Yuanyuan Zhang Yangyang Sun Ziyang Xie Yu Luo Qiyuan Long Jiahui Feng Xueqing Liu Bi Wang Dujun He Junxia Ren Peizhen Guo Junwei Xing Liqiang He alisdair rfernie Wei Chen Xianqing Liu Yuehua Luo Cheng Jin Jie Luo Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China College of Tropical Crops Hainan UniversityHaikou 570228China Max Planck Institute of Molecular Plant Physiology 14476 Potsdam-GolmGermany National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China

Grain essential amino acid(EAA)levels contribute to rice nutritional quality.However,the molecular mechanisms underlying EAA accumulation and natural variation in rice grains remain unclear.Here we report the identification of a previously unrecognized auxin influx carrier subfamily gene,OsAUX5,which encodes an amino acid transporter that functions in uptake of multiple amino acids.We identified an elite haplotype of Pro::OsAUX5^(Hap2) that enhances grain EAA accumulation without an apparent negative effect on agronomic traits.Natural variations of OsAUX5 occur in the cis elements of its promoter,which are differentially activated because of the different binding affinity between OsWRKY78 and the W-box,contributing to grain EAA variation among rice varieties.The two distinct haplotypes were shown to have originated from different Oryza rufipogon progenitors,which contributed to the divergence between japonica and indica.Introduction of the indica-type Pro::OsAUX5^(Hap2) genotype into japonica could significantly increase EAA levels,indicating that indica-type Pro::OsAUX5^(Hap2) can be utilized to increase grain EAAs of japonica varieties.Collectively,our study uncovers an WRKY78–OsAUX5-based regulatory mechanism controlling grain EAA accumulation and provides a potential target for breeding EAA-rich rice.

关键词： amino acid natural variation transcriptional regulation Oryza sativa

Promoter replacement of ANT1 induces anthocyanin accumulation and triggers the shade avoidance response through developmental, physiological and metabolic reprogramming in tomato

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Horticulture Research 2023年第2期10卷 28-40页

作者： João Victor Abreu Cerqueira Feng Zhu Karoline Mendes Adriano Nunes-Nesi Samuel Cordeiro Vitor Martins Vagner Benedito alisdair rfernie Agustin Zsögön Departamento de Biologia Vegetal Universidade Federal de ViçosaViçosa 36570-900 MGBrazil National R&D Center for Citrus Preservation Key Laboratory of Horticultural Plant BiologyMinistry of EducationHuazhong Agricultural University430070 WuhanChina Max-Planck-Institute for Molecular Plant Physiology 14476 PotsdamGermany Division of Plant&Soil Sciences West Virginia UniversityMorgantownWV 26506USA

The accumulation of anthocyanins is a well-known response to abiotic stresses in many plant species.However,the effects of anthocyanin accumulation on light absorbance and photosynthesis are unknown.Here,we addressed this question using a promoter replacement line of tomato constitutively expressing a MYB transcription factor(ANTHOCYANIN1,ANT1)that leads to anthocyanin accumulation.ANT1-overexpressing plants displayed traits associated with shade avoidance response:thinner leaves,lower seed germination rate,suppressed side branching,increased chlorophyll concentration,and lower photosynthesis rates than the wild type.Anthocyanin-rich leaves exhibited higher absorbance of light in the blue and red ends of the spectrum,while higher anthocyanin content in leaves provided photoprotection to high irradiance.Analyses of gene expression and primary metabolites content showed that anthocyanin accumulation produces a reconfiguration of transcriptional and metabolic networks that is consistent with,but not identical to those described for the shade avoidance response.Our results provide novel insights about howanthocyanins accumulation affects the trade-off between photoprotection and growth.

关键词： metabolic expressing programming

NET位点决定了大米的口感、蒸煮和营养品质

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Science Bulletin 2022年第20期67卷 2045-2049,M0003页

作者：李宇飞杨壮杨陈坤刘振寰沈双欠占传松吕媛媛张凤李康施宇恒周骏杰刘贤青房传营 alisdair rfernie 李洁罗杰 National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China College of Tropical Crops Hainan UniversityHaikou 570228China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany John Innes Centre Norwich Research ParkNorwichNR47UHUK

水稻是重要的粮食作物,为全球一半人口提供热量需求.随着经济发展和生活水平的提高,人们对大米的需求逐渐从“吃饱”转变为“吃好”,美味营养的品种是后者的必要条件.以往的研究大多都集中在提高大米的食味和蒸煮品质上以满足口感需求,... 详细信息

水稻是重要的粮食作物,为全球一半人口提供热量需求.随着经济发展和生活水平的提高,人们对大米的需求逐渐从“吃饱”转变为“吃好”,美味营养的品种是后者的必要条件.以往的研究大多都集中在提高大米的食味和蒸煮品质上以满足口感需求,但改善水稻综合营养的研究仍然是零散的.本研究通过正向遗传学手段采用代谢物全基因组关联分析结合代谢物数量性状位点分析方法,定位到一个调控水稻谷粒多种营养代谢物积累的数量性状位点,并命名为NET.实验证明NET位点可以同时调控大米的食用、蒸煮以及营养品质.结合NET位点的驯化路线以及驯化过程中营养代谢物的变化,发现水稻育种过程中食味品质和营养品质没有得到有效的平衡.因此,为满足“吃好”的目标,有必要考虑到育种过程中营养物质的变化,达到两全其美,而NET位点可能是实现这一目标的重要的遗传位点之一.该研究结果阐明了水稻驯化过程中营养代谢物变化的遗传机制,为获得理想的高食用品质、高营养品种提供了可能的育种方案.

关键词：数量性状位点育种方案育种过程理想的高营养品质水稻育种蒸煮品质食味

Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy“Newhall”navel orange mutant

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Horticulture Research 2020年第1期7卷 2167-2184页

作者： Haoliang Wan Hongbo Liu Jingyu Zhang Yi Lyu Zhuoran Li Yizhong He Xiaoliang Zhang Xiuxin Deng Yariv Brotman alisdair rfernie Yunjiang Cheng Weiwei Wen Key Laboratory of Horticultural Plant Biology(MOE) College of Horticulture and Forestry SciencesHuazhong Agricultural University430070 WuhanChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University430070 WuhanChina Key Laboratory for Space Bioscience and Biotechnology School of Life SciencesNorthwestern Polytechnical UniversityYouyi Xilu 127Xi’an 710072 ShaanxiChina Department of Life Sciences Ben-Gurion University of the NegevBeershebaIsrael Max-Planck-Institute of Molecular Plant Physiology Am Muehlenberg 114476 PotsdamGermany

Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits.Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants;however,the specific alterations concerning lipids have not been clarified thus far.Here,we conducted a comprehensive,timeresolved lipidomic,and transcriptomic analysis on the“Newhall”navel orange(WT)and its glossy mutant(MT)“Gannan No.1”.The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT.Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids.Moreover,the high accumulation of jasmonic acid(JA)in MT was possibly due to the need to maintain plastid lipid homeostasis,as the expression levels of two significantly upregulated lipases(CsDAD1 and CsDALL2)were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content.Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.

关键词： orange carbon concerning

Diel dynamics of multi-omics in elkhorn fern provide new insights into weak CAM photosynthesis

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Plant Communications 2023年第5期4卷 208-222页

作者： Cheng Li Wenjie Huang Xiaoxu Han Guohua Zhao Wenyang Zhang Weijun He Bao Nie Xufeng Chen Taijie Zhang Wenhui Bai Xiaopeng Zhang Jingjing He Cheng Zhao alisdair rfernie Timothy JTschaplinski Xiaohan Yang Shijuan Yan Li Wang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization Agro-biological Gene Research CenterGuangdong Academy of Agricultural SciencesGuangzhouChina Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innovation Oak Ridge National LaboratoryOak RidgeTN 37831USA Max Planck Institute of Molecular Plant Physiology Am Muhlenberg 114476 Potsdam-GolmGermany Key Laboratory of Southern Subtropical Plant Diversity Fairy Lake Botanical GardenShenzhen&Chinese Academy of ScienceShenzhenChina Institute of Plant Protection Guangdong Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South ChinaMinistry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina Kunpeng Institute of Modern Agriculture at Foshan Chinese Academy of Agricultural SciencesFoshanChina

Crassulacean acid metabolism(CAM)has high water-use efficiency(WUE)and is widely recognized to have evolved from C3 photosynthesis.Different plant lineages have convergently evolved CAM,but the molecular mechanism that underlies C3-to-CAM evolution remains to be clarified.Platycerium bifurcatum(elkhorn fern)provides an opportunity to study the molecular changes underlying the transition from C3 to CAM photosynthesis because both modes of photosynthesis occur in this species,with sporotrophophyll leaves(SLs)and cover leaves(CLs)performing C3 and weak CAM photosynthesis,respectively.Here,we report that the physiological and biochemical attributes of CAM in weak CAM-performing CLs differed from those in strong CAM species.We investigated the diel dynamics of the metabolome,proteome,and transcriptome in these dimorphic leaves within the same genetic background and under identical environmental conditions.We found that multi-omic diel dynamics in P.bifurcatum exhibit both tissue and diel effects.Our analysis revealed temporal rewiring of biochemistry relevant to the energy-producing pathway(TCA cycle),CAM pathway,and stomatal movement in CLs compared with SLs.We also confirmed that PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE(PPCK)exhibits convergence in gene expression among highly divergent CAM lineages.Gene regulatory network analysis identified candidate transcription factors regulating the CAM pathway and stomatal movement.Taken together,our results provide new insights into weak CAM photosynthesis and new avenues for CAM bioengineering.

关键词： Platycerium bifurcatum crassulacean acid metabolism multi-omics transcription factor PPCK convergent evolution

CsbZIP1-CsMYB12 mediates the production of bitter-tasting flavonols in tea plants (Camellia sinensis) through a coordinated activator–repressor network

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Horticulture Research 2021年第1期8卷 1495-1512页

作者： Xuecheng Zhao Xiangsheng Zeng Ning Lin Shuwei Yu alisdair rfernie Jian Zhao State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University230036 HefeiChina College of Agronomy Anhui Agricultural University230036 HefeiChina Max-Planck-Institute of Molecular Plant Physiology Am Mühlenberg 114476 Potsdam-GolmGermany

Under high light conditions or UV radiation,tea plant leaves produce more flavonols,which contribute to the bitter taste of tea;however,neither the flavonol biosynthesis pathways nor the regulation of their production are well understood.Intriguingly,tea leaf flavonols are enhanced by UV-B but reduced by shading treatment.CsFLS,CsUGT78A14,CsMYB12,and CsbZIP1 were upregulated by UV-B radiation and downregulated by shading.CsMYB12 and CsbZIP1 bound to the promoters of CsFLS and CsUGT78A14,respectively,and activated their expression individually.CsbZIP1 positively regulated CsMYB12 and interacted with CsMYB12,which specifically activated flavonol biosynthesis.Meanwhile,CsPIF3 and two MYB repressor genes,CsMYB4 and CsMYB7,displayed expression patterns opposite to that of CsMYB12.CsMYB4 and CsMYB7 bound to CsFLS and CsUGT78A14 and repressed their CsMYB12-activated expression.While CsbZIP1 and CsMYB12 regulated neither CsMYB4 nor CsMYB7,CsMYB12 interacted with CsbZIP1,CsMYB4,and CsMYB7,but CsbZIP1 did not physically interact with CsMYB4 or CsMYB7.Finally,CsPIF3 bound to and activated CsMYB7 under shading to repress flavonol biosynthesis.These combined results suggest that UV activation and shading repression of flavonol biosynthesis in tea leaves are coordinated through a complex network involving CsbZIP1 and CsPIF3 as positive MYB activators and negative MYB repressors,respectively.The study thus provides insight into the regulatory mechanism underlying the production of bitter-tasting flavonols in tea plants.

关键词： coordinated media opposite