Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy“Newhall”navel orange mutant

作     者：Haoliang Wan Hongbo Liu Jingyu Zhang Yi Lyu Zhuoran Li Yizhong He Xiaoliang Zhang Xiuxin Deng Yariv Brotman Alisdair RFernie Yunjiang Cheng Weiwei Wen Haoliang Wan;Hongbo Liu;Jingyu Zhang;Yi Lyu;Zhuoran Li;Yizhong He;Xiaoliang Zhang;Xiuxin Deng;Yariv Brotman;Alisdair R.Fernie;Yunjiang Cheng;Weiwei Wen

作者机构：Key Laboratory of Horticultural Plant Biology(MOE)College of Horticulture and Forestry SciencesHuazhong Agricultural University430070 WuhanChina National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University430070 WuhanChina Key Laboratory for Space Bioscience and BiotechnologySchool of Life SciencesNorthwestern Polytechnical UniversityYouyi Xilu 127Xi’an 710072 ShaanxiChina Department of Life SciencesBen-Gurion University of the NegevBeershebaIsrael Max-Planck-Institute of Molecular Plant PhysiologyAm Muehlenberg 114476 PotsdamGermany 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2020年第7卷第1期

页      面：2167-2184页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：financed by funding from the National Natural Science Foundation of China(31772261) the Huazhong Agricultural University Scientific&Technological Self-Innovation Foundation granted to W.W 

主　　题：orange carbon concerning 

摘      要：Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh *** reports have indicated the modification of alternative branch pathways in epicuticular wax mutants;however,the specific alterations concerning lipids have not been clarified thus ***,we conducted a comprehensive,timeresolved lipidomic,and transcriptomic analysis on the“Newhallnavel orange(WT)and its glossy mutant(MT)“Gannan No.1.The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in *** analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane ***,the high accumulation of jasmonic acid(JA)in MT was possibly due to the need to maintain plastid lipid homeostasis,as the expression levels of two significantly upregulated lipases(CsDAD1 and CsDALL2)were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA *** results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit.