Elucidation of the melitidin biosynthesis pathway in pummelo

作     者：Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou Ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu Alisdair RFernie Jie Luo Shuangqian Shen;Shouchuang Wang;Chenkun Yang;Chao Wang;Qianqian Zhou;Shen Zhou;Ran Zhang;Yufei Li;Zixuan Wang;Liupan Dai;Wenjv Peng;Yingchen Hao;Hao Guo;Guangping Cao;Xianqing Liu;Fan Yao;Qiang Xu;Alisdair R.Fernie;Jie Luo

作者机构：Sanya Nanfan Research Institute of Hainan UniversityHainan UniversitySanya 572025China National Key Laboratory of Crop Genetic ImprovementNational Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan LaboratoryCollege of Life Science and TechnologyCollege of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Horticultural Plant Biology of Ministry of EducationHuazhong Agricultural UniversityWuhan 430070China Max Planck Institute of Molecular Plant PhysiologyPotsdam-Golm 14476Germany Centre of Plant Systems Biology and BiotechnologyPlovdiv 4000Bulgaria Yazhouwan National LaboratorySanya 572025China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第11期

页      面：2505-2518页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Key R&D Program of China (2021YFA0909600) the National Science Fund for Distinguished Young Scholars of China (31625021 to J.L) the“111”Project (No.D20024) the Young Elite Scientists Sponsorship Program by CAST (2019QNRC001) the National Postdoctoral Program for Innovative Talents (BX20220097) the China Postdoctoral Science Foundation (2022M710991) the Hainan Provincial Natural Science Foundation of China (323MS019) the Hainan Postdoctoral Grant Project (2022-BH-14) the Hainan Provincial Academician Innovation Platform Project (HD-YSZX-202003,HD-YSZX-202004) the Hainan University Startup Fund (KYQD (ZR)1866,KYQD (ZR)1916) 

主　　题：bioactive metabolites biosynthetic pathway flavonoids melitidin natural variation 

摘      要：Specialized plant metabolism is a rich resource of compounds for drug *** acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully ***,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2Rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of ***,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug ***-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant *** study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites.