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Cyclooxygenase-2 polymorphisms and susceptibility to gastric carcinoma: A meta-analysis

World Journal of Gastroenterology 2010年第43期16卷 5510-5517页

作者： Jia-Li Liu, Zhen-Ning Wang, department of Surgical Oncology and General Surgery, the First affiliated hospital of china Medical university, Shenyang 110001, Liaoning province, china Yuan Liang, department of Oncology, the Shengjing affiliated hospital of china Medical university, Shenyang 110001, Liaoning province, china Xin Zhou, department of Obstetrics and Gynecology, the Shengjing affiliated hospital of china Medical university, Shenyang 110001, Liaoning province, china Li-Li Xing, department of Oncology, the Fourth affiliated hospital of china Medical university, Shenyang 110032, Liaoning province, china department of Surgical Oncology and General Surgery the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China. josieon826@***

AIM: To investigate the association of the cyclooxygenases-2 (COX-2) polymorphisms and susceptibility to gastric cancer (GC) by means of meta-analysis. METHODS: publications addressing the association between polymorphisms of COX-2 and susceptibility to GC were selected from the MEDLINE, EMBASE and CBmdisc databases. Data was extracted from the studies by 2 independent reviewers. The meta-analyses were performed by revMan 5.0.23. From these data, odds ratio (Or) with 95% confidence interval (CI) was ***: Ten studies were retrieved reporting a total of 11 COX-2 polymorphisms. Carriers of -765C, -1195A, -1290G, *2430T alleles and *429TT genotype revealed increased risk for GC (Or = 1.71, 95% CI: 1.01-2.90, p = 0.05; Or = 1.58, 95% CI: 1.05-2.38, p = 0.03; Or = 1.55, 95% CI: 1.01-2.39, p = 0.05; Or = 2.62, 95% CI: 1.20-5.73, p = 0.02 and Or = 0.74, 95% CI: 0.59-0.95, p = 0.02, respectively). CONCLUSION: The -765C, -1195A, -1290G, *2430T alleles and *429TT genotype of COX-2 polymorphisms were determined a significant association with susceptibility to GC.

关键词： Cyclooxygenases-2 Gastric cancer polymorphisms Susceptibility Meta-analysis

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Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats

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Acta pharmacologica Sinica 2004年第05期25卷 69-74页

作者： Yan-ting WANG2, Shu HAN2, Kai-hua ZHANG2, Yu JIN3, Xiao-ming XU2,4, pei-hua LU2,52department of Neurobiology, Shanghai Second Medical university, Shanghai 200025, china 3department of Surgery,Xinhua hospital affiliated to Shanghai Second Medical university 4Kentucky Spinal Cord Injury research Center,department of Neurological Surgery, university of Louisville School of Medicine, Louisville, KY, 40292, usa department of Neurobiology Shanghai Second Medical University Shanghai 200025 China

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM ex-pression and cellular localization were examined using Northern blot, rT-pCr, immunohistochemistry and immun-ofluorescence double-labeling methods. rESULTS: HB-GAM mrNA was significantly upregulated in spinal cordtissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d,peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was co-localized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinaltransection. CONCLUSION: SCI induces HB-GAM expression at both mrNA and protein levels in areas close tothe injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process ofinjury and/or repair following SCI.

关键词： heparin-binding growth-associated molecule spinal cord injuries immunohistochemistry reversetranscriptase polymerase chain reaction astrocytes neurons

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Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

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Neural regeneration research 2006年第7期1卷 618-621页

作者： Yong Man1, Jianbin Li2, Bo Yang3, Ji Ma1 1department of Cerebral Surgery, the Second people’s hospital of Zhengzhou, Zhengzhou 450000, Henan province, china 2red Cross Blood Center of Henan province, Zhengzhou 450000, Henan province, china 3department of neurosurgery, the First affiliated hospital of Zhengzhou university, Zhengzhou 450000, Henan province, china department of Cerebral Surgery The Second People's Hospital of Zhengzhou Zhengzhou 450000 Henan Province China red Cross Blood Center of Henan province Zhengzhou 450000 Henan Province China department of neurosurgery Hospital of Zhengzhou University Zhengzhou 450000 Henan Province China

BACKGrOUnd: Transplanted mononuclear cell (MNC) of umbilical blood can survive in central nervous system (CNS) of host through blood brain barrier, differentiate into nerve cells, migrate to damaged site and integrate morphological structure and function with nerve cells of host so as to improve deficiencies of sensatory function, motor function and cognitive function and influence on stroke sequela. OBJECTIVE: To observe the vein transplantation of human umbilical cord blood stem cells (HUCBSC) for improving neurological function, limb function and activity of daily living of patients with stroke and evaluate the reliability. DESIGN: Self-controlled study. SETTING: department of neurosurgery, the Second people's hospital of Zhengzhou City; red-crossed Blood Center of Henan province; department of neurosurgery, the Fist affiliated hospital of Zhengzhou university. pArTICIpANTS: A total of 10 patients with stroke sequela were selected from department of Cerebral Surgery, the Second people's hospital of Zhengzhou City from April to December 2005. There were 9 males and 1 female aged from 35 to 75 years with the mean age of 56 years. All of them were diagnosed with CT and MrI examination and coincidence with diagnostic criteria of stroke established by the Fourth National Academic Meeting for Cerebrovascular Disease. All patients provided informed consent. METHODS: 80-140 mL umbilical blood of term birth of newborn was selected hermetically and maintained in sterile plastic bag. And then, the blood was centrifugated at the speed of 1 500 r/min for 30 minutes at 22 ℃ in order to separate MNC, i.e., HUCBSC. In addition, after final diagnosis during hospitalization, stroke patients were perfused with HUCBSC through superficial vein of back of the hand. Each patient was averagely perfused with 6 portions of HUCBSC (cellular numbers ≥ 1×108/portion) and the interval between each portion was 1-7 days with the mean interval of 4 days. MAIN OUTCOME MEASUrES: ① Neurological fun

关键词： cell stem Vein transplantation using human umbilical cord blood stem cells in the treatment of stroke sequela

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Influence of rotating magnetic field on cerebral infarction volume, cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats

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Neural regeneration research 2006年第9期1卷 777-780页

作者： Xiaohong Liu1, Zhiqiang Zhang2, Lixin Zhang3 1Liaoning Disabled Children’s rehabilitation Center, Shenyang 110015, Liaoning province, china 2department of rehabilitation, Second hospital affiliated to china Medical university, Shenyang 110003, Liaoning province, china 3department of rehabilitation and physiotherapy, First hospital affiliated to china Medical university, Shenyang 110001, Liaoning province, china Liaoning Disabled Children's rehabilitation Center Shenyang 110015 Liaoning Province China department of rehabilitation China Medical University Shenyang 110003 Liaoning Province China department of rehabilitation and physiotherapy China Medical University Shenyang 110001 Liaoning Province China

BACKGrOUnd: It has shown that magnetic field can improve blood circulation, decrease blood viscosity, inhibit free radicals, affect Ca2+ flow in nerve cells, control inflammatory and immunological reaction, and accelerate nerve cell regeneration. In addition, protective effect of magnetic field, which acts as an iatrophysics, on ischemic brain tissues has been understood gradually. OBJECTIVE: To investigate the effects of rotating magnetic field (rMF) on volume of cerebral infarction, cerebral edema and metabolism of free radicals in rats after cerebral ischemia/reperfusion injury. DESIGN: randomized controlled animal study. SETTING: rehabilitation Center of disabled children, Liaoniang; department of rehabilitation, the Second affiliated hospital, china Medical university; department of rehabilitation physiotherapy, the First affiliated hospital, china Medical university. MATErIALS: A total of 70 healthy Wistar rats aged 18-20 weeks of both genders were selected and randomly divided into 3 groups: sham operation group with 12 rats, control group with 20 rats and treatment group with 38 rats. The treatment group included 4 time points: immediate reperfusion with 6 ones, 6-hour reperfusion with 20 ones, 12-hour reperfusion with 6 ones and 18-hour reperfusion with 6 rats. Main instruments were detailed as follows: magnetic head of rotating magnetic device was 6 cm in diameter; magnetic induction intensity at the surface of magnetic head was 0.25 T in silence; the maximal magnetic induction intensity was 0.09 T at the phase of rotation; the average rotating speed was 2500 r per minute. METHODS: The experiment was carried out in the china Medical university in March 2003. Focal cerebral ischemic animal models were established with modified Longa’s method. Operation was the same in the sham operation, but the thread was inserted as 10 mm. Neurologic impairment was assessed with 5-rating method to screen out cases. Those survivals with grade 1 and grade 2 after ischemia f

关键词： cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats free

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Urotensin Ⅱ accelerates cardiac fibrosis and hypertrophy of rats induced by isoproterenol

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Acta pharmacologica Sinica 2007年第1期28卷 36-43页

作者： Yong-gang ZHANG~(2,5) Yu-guang LI~2 Bao-guo LIU~3 rui-hong WEI~2 Dong-ming WANG~2 Xue-rui TAN~2 Ding-fang BU~4 Yong-zheng pANG~4 Chao-shu TANG~42.department of Cardiovascular Diseases,First affiliated hospital,Shantou university Medical College,Shantou 515041,china 3 The affiliated hospital,Medical School of Hebei university of Engineering,Hebei 056029,china,4 Institute of Cardiovascular research,peking university First hospital,Beijing 100034,china department of Cardiovascular Diseases First Affiliated Hospital Shantou University Medical College Shantou China The affiliated hospital Medical School of Hebei University of Engineering Hebei China Institute of Cardiovascular research Peking University First Hospital Beijing China

Aim:To study whether urotensin II（UII）,a potent vasoconstrictive peptide,isinvolved in the development of cardiac hypertrophy and fibrogenesis of ratsinduced by isoproterenol（ISO）.Methods:Thirty male Wistar rats were randomlydivided into 3 *** 1 was the healthy control group,group 2 was the ISOgroup,and group 3 was the ISO+UII *** groups 2 and 3,ISO(5 mg-kg-1.d-1)wasgiven（sc）once daily for 7 *** 3 was also given UII in the first day [3 nmol/kg（5μg/kg）,iv],followed by sc（1.5 μg/kg）twice *** 1 received 0.9% *** receptor（UT）mrNA expression was determined by *** contents ofUII and angiotensin II（Ang II）were determined by *** vitro,the effects of UII on DNA/collagen synthesis of cardiac fibroblasts were deter-mined by [3H]thymidine/[3H]proline ***:The ratio of heartweight/body weight,plasma lactate dehydrogenase activity,myocardialmalondialdehyde and hydroxyproline concentration increased significantly in theISO group,as well as UT mrNA expression,plasma and cardiac UII and ventricu-lar Ang II,compared with the control group（p1）.ISO induced significantmyocardial ***,UII+ISO co-treatment significantly increasedthe changes of biochemical markers of injury and the degree of cardiac hypertro-phy and *** vitro,5×10～9-5×10-7mol/L UII stimulated[3H]thymidine/[3H]proline incorporation into cardiac fibroblasts in a dose-dependent manner（p1）.Conclusion:These results suggest that UII was involved in the development ofcardiac fibrosis and hypertrophy by synergistic effects with ISO.

关键词： cardiac remodeling cardiac fibrosis Gpr14 receptor urotensin II collagen

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Diagnosis and excision of breast multi-focal lesions by ultrasound-guided vacuum-assisted biopsy:a comparative evaluation

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Journal of pharmaceutical Analysis 2010年第3期22卷 208-211页

作者： Tie-Qiang Bi1,Chang-Ling ren2,prakash Chaudhary1,Xin-Hua Liao1,Xiang-Ming Che1 1.department of Surgery,the First affiliated hospital,Medical School of Xi’an Jiaotong university,Xi’an 710061 2.department of Surgery,Laizhou people’s hospital,Laizhou 261400,china department of Surgery First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China department of Surgery Laizhou People's Hospital Laizhou 261400 China

Objective To compare and evaluate the efficacy of diagnosis and excision for appropriately selected breast multi-focal lesions and solitary lesion by ultrasound-guided vacuum-assisted biopsy(UGVAB).Methods Among 392 appropriately selected patients,187 patients with multi-focal lesions and 205 patients with solitary lesion were treated by the 8-gauge UGVAB from May 2007 to June *** lesions were removed as completely as *** patients with benign pathology underwent physical and ultrasound examinations at one week and 6 months after *** During the procedure,only three patients had vasovagal syncope and twenty others complained of other intraoperative *** accurate pathological diagnosis was obtained in all *** was no apparent false-negative result among the 696 lesions with benign pathology at a follow-up of 6 months after *** rates of malignant or premalignant pathology,postoperative complications and residual lesions in patients with multi-focal lesions were higher than those in patients with solitary *** each lesion was considered as a subject of study,there was no significant difference between the two *** UGVAB is an effective method for diagnosis and excision of appropriately selected breast multi-focal lesions and can be used routinely.

关键词： vacuum-assisted biopsy ultrasound-guided breast multi-focal lesions

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plasma hemoglobin concentration was related to estimated glomerular filtration rate in elderly patients with ischemic cardiomyopathy

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Journal of Geriatric Cardiology 2008年第3期5卷 150-154页

作者： Gang Li,~1 Zhihua Wang,~1 Canjing Zhang,~2 Yang Wang~1 1 Division of Cardiology, department of Geriatrics, The First affiliated hospital, Chongqing Medical university, Chongqing 400016, china 2.department of Function Examination, Chongqing Xijiao hospital, Chongqing 400050, china department of Geriatrics First Affiliated Hospital Chongqing Medical University Chongqing 400016 China department of Function Examination Chongqing Xijiao Hospital Chongqing 400050 China

Objectives To study the relationship between plasma hemoglobin concentration and estimated glomerular filtration rate （eGFr） in elderly patients with ischemic cardiomyopathy （ICM）.Methods Clinical data of patients with coronary heart disease who were discharged from The First affiliated hospital,Chongqing Medical university between 2005 and 2007 were analyzed retrospectively. Echocardiography results,plasma hemoglobin and creatinine concentration were abstracted from the medical *** study included 235 Chinese Hart patients with age 60 years and older with angiography confirmed coronary heart disease,silent myocardial ischemia or angina pectoris,of whom 154 had ICM defined as left ventricular end-diastolic diameter （LVDd）,male≥56 mm,female≥51 mm （63. 51±7.70 mm） measured by M-mode *** differences in plasma hemoglobin concentration were analyzed retrospec- tively between patients with and without ICM,and between patients with an eGFrp>-1p>·1.73mp>-2p> and those with an eGFr≥60 ml·minp>-1p>·1.73mp>-2p>.results There were no significant differences in plasma hemoglobin concentration and eGFr between ICM and non-ICM group (118.49±20.52 g·Lp>-1p> vs.115.80±23.32 g·Lp>-1p> and 75.13±24.21 ml·minp>-1p>·1.73mp>-2p> vs.79.09±28.41 ml·minp>- 1p>·1.73mp>-2p>,respectively,both p>0.05).However,in both ICM and non-ICM groups,plasma hemoglobin concentration was lower in those with an eGFrp>-1p>·1.73mp>-2p> compared with compared with those with an eGFr≥60 ml·minp>-1p>·1.73mp>-2p> group (112. 29±18.61 g·Lp>-1p> vs.119.92±20.74Lp>-1p>,pplasma hemoglobin concentration was related positively to *** There were no significant changes in plasma hemoglobin concentration and eGFr;however,plasma hemoglobin concentration was related to eGFr significantly positively in elderly patients with ICM due to corona

关键词： ischemic cardiomyopathy coronary heart disease silent myocardial ischemia angina pectoris estimated glomerular filtration rate plasma hemoglobin concentration

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CT examination, clinical situation and experimental characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency

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Neural regeneration research 2006年第9期1卷 801-805页

作者： Zhiqing Lin1, Feng Fang1, Min Chen2, Guoxiang Cai3 1department of pediatrics, Tongji hospital affiliated to Tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, Hubei province, china 2department of pediatrics, 3department of radiation Medicine, Zhongshan hospital affiliated to Xiamen university, Xiamen 361004, Fujian province, china department of pediatrics Tongji Hospital Huazhong University of Science and Technology Wuhan 430030 Hubei Province China department of pediatrics Zhongshan Hospital Xiamen University Xiamen 361004 Fujian Province China department of radiation Medicine Zhongshan Hospital Xiamen University Xiamen 361004 Fujian Province China

BACKGrOUnd: Delayed vitamin K deficiency is characterized by acute onset, severe illness and high fatality rate. 33%-50% survivors accompany with other various nervous system sequelas. Therefore, diagnosis and treatment of intracranial hemorrhage in time become a key factor for improving healing rate and reducing fatality rate and incidence of sequela. OBJECTIVE: To investigate the clinical situation, experimental characteristics, CT examination and terminative characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency. DESIGN: Case analysis. SETTING: department of pediatrics, Zhongshan hospital affiliated to Xiamen university. pArTICIpANTS: A total of 17 infants with intracranial hemorrhage induced by delayed vitamin K deficiency aged 1-3 months including 11 boys and 6 girls were selected from department of pediatrics, Zhongshan hospital affiliated to Xiamen university from January 1994 to December 2005. All infants had drowsiness, rejective milk, spiting milk, gaze of both eyes, tic, coma, full anterior fontanelle, high muscular tension and cerebral hernia, etc. Experimental examination demonstrated that infants had anemia at various degrees; prothrombin time and partial thromboplastin time were prolonged; platelet count was normal. CT examination indicated that screenages of subarachnoid hemorrhage, subdural hematoma, cerebral parenchyma hemorrhage and intraventricular hemorrage were changed. Hemorrhage was stopped by the application of vitamin K. All patients provided informed consent. METHODS: ① Clinical situation and physical sign of infants were observed after hospitalization and scanned with rapid spiral CT scanning system. The thickness and average space of layers were 8-10 mm and the scanning time was 5 s with window width of 30-80 Hu and window position of 28-35 Hu. ② After hospitalization, four items of blood coagulation was measured with Futura meter and biochemical indexes of blood, such as serum calcium, serum alka

关键词： clinical situation and experimental characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency CT examination

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A portability Multifunctional Neurobehavioral Testing Device:Using for rat Models

A Portability Multifunctional Neurobehavioral Testing Device...

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2011年浙江省神经外科学学术年会

作者： department of neurosurgery,the 2nd affiliated hospital,School of Medicine,zhejiang university Shi Jian Zhang Hong Weiming Fu

At present,laboratorial neurobehavioral research could be practiced by observing experimental animal’ changes of nature behavior subjectively,or by evaluating animal performance on some professional neurobehavioral equipments *** neurobehavioral equipments for laboratorial rat experiment are numerous,and they have different functions and were designed for different neurobehavioral *** their application exist some disadvantages,such as complex structure,costly price,difficulty of carrying, and only one kind of test can be performed on one set of special *** solve this problem,we reviewed some existent neurobehavioral test projects,then designed and made a set of portable multifunctional equipment. According installation and geometrical change of this equipment,five different tests were able to be practiced, which are beam walking test,beam balance test,slope test,open field activity assay and memory test, evaluate the systemized neural function for experimental rat.

关键词： neurobehavioral test rat

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Suppression of Type 1 Insulin-like Growth Factor receptor Expression by Small Interfering rNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice

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Acta Biochimica et Biophysica Sinica 2007年第2期39卷 137-147页

作者： Jianfang QIAN~1 Aiqiang DONG~1 Minjian KONG~1 Zhiyuan MA~2 Junqiang FAN~1 and Guanyu JIANG~3 ~1department of Cardiothoracic Surgery,Second affiliated hospital,School of Medicine,zhejiang university,Hangzhou 310009,china ~2department of Cardiovascular Surgery,Shanghai Jiaotong university affiliated First people's hospital,Shanghai 200080,china ~3department of Emergency,Second affiliated hospital School of Medicine,zhejiang university,Hangzhou 310009,china department of Cardiothoracic Surgery Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Cardiovascular SurgeryShanghai Jiaotong University Affiliated First People's HospitalShanghai 200080China De

Cancer invasion and metastasis, involving a variety of pathological processes andcytophysiological changes,contribute to the high mortality of lung *** type 1 insulin-like growthfactor receptor (IGF-1r),associated with cancer progression and invasion,is a potential anti-invasion andanti-metastasis target in lung *** inhibit the invasive properties of lung cancer cells,we successfullydown-regulated IGF-1r gene expression in A549 human lung cancer cells by small interfering rNA (sirNA)technology,and evaluated its effects on invasion-related gene expression,tumor cell in vitro invasion,andmetastasis in xenograft nude mice.A549 cells transfected with a plasmid expressing hairpin sirNA forIGF-1r showed a significantly decreased IGF-1r expression at the mrNA level as well as the *** biological assays,transfected A549 cells showed a significant reduction of cell-matrix adhesion,migration and *** with these results,we found that down-regulation of IGr-1rconcomitantly accompanied by a large reduction in invasion-related gene expressions,including MMp-2,MMp-9,u-pA,and IGF-1r specific downstream *** tail vein injections of plasmid expressinghairpin sirNA for IGF- 1r significantly inhibited the formation of lung metastases in nude *** resultsshowed the therapeutic potential of sirNA as a method for gene therapy in inhibiting lung cancer invasionand metastasis.

关键词： lung cancer invasion metastasis type 1 insulin-like growth factor receptor small interfering rNA

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