Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by Small Interfering RNA Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice

作     者：Jianfang QIAN~1 Aiqiang DONG~1 Minjian KONG~1 Zhiyuan MA~2 Junqiang FAN~1 and Guanyu JIANG~3 ~1Department of Cardiothoracic Surgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China ~2Department of Cardiovascular Surgery,Shanghai Jiaotong University Affiliated First People's Hospital,Shanghai 200080,China ~3Department of Emergency,Second Affiliated Hospital School of Medicine,Zhejiang University,Hangzhou 310009,China 

作者机构：Department of Cardiothoracic SurgerySecond Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Cardiovascular SurgeryShanghai Jiaotong University Affiliated First People's HospitalShanghai 200080China De 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2007年第39卷第2期

页      面：137-147页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by a grant from the Natural Science Foundation of zhejiang Province(Y-204317) 

主　　题：lung cancer invasion metastasis type 1 insulin-like growth factor receptor small interfering RNA 

摘      要：Cancer invasion and metastasis, involving a variety of pathological processes andcytophysiological changes,contribute to the high mortality of lung *** type 1 insulin-like growthfactor receptor (IGF-1R),associated with cancer progression and invasion,is a potential anti-invasion andanti-metastasis target in lung *** inhibit the invasive properties of lung cancer cells,we successfullydown-regulated IGF-1R gene expression in A549 human lung cancer cells by small interfering RNA (siRNA)technology,and evaluated its effects on invasion-related gene expression,tumor cell in vitro invasion,andmetastasis in xenograft nude mice.A549 cells transfected with a plasmid expressing hairpin siRNA forIGF-1R showed a significantly decreased IGF-1R expression at the mRNA level as well as the *** biological assays,transfected A549 cells showed a significant reduction of cell-matrix adhesion,migration and *** with these results,we found that down-regulation of IGR-1Rconcomitantly accompanied by a large reduction in invasion-related gene expressions,including MMP-2,MMP-9,u-PA,and IGF-1R specific downstream *** tail vein injections of plasmid expressinghairpin siRNA for IGF- 1R significantly inhibited the formation of lung metastases in nude *** resultsshowed the therapeutic potential of siRNA as a method for gene therapy in inhibiting lung cancer invasionand metastasis.