Urotensin Ⅱ accelerates cardiac fibrosis and hypertrophy of rats induced by isoproterenol
Urotensin Ⅱ accelerates cardiac fibrosis and hypertrophy of rats induced by isoproterenol作者机构：Department of Cardiovascular Diseases First Affiliated Hospital Shantou University Medical College Shantou China The Affiliated Hospital Medical School of Hebei University of Engineering Hebei China Institute of Cardiovascular Research Peking University First Hospital Beijing China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2007年第28卷第1期
页 面：36-43页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Project supported by the China Postdoctoral Science Foundation(No 2003033439) supported by the National Natural Science Foundation of China(No 30470730) the Medical Science Foundation of Hebei Province Health Department(No 2 K010)
主 题：cardiac remodeling cardiac fibrosis GPR14 receptor urotensin II collagen
摘 要：Aim:To study whether urotensin II（UII）,a potent vasoconstrictive peptide,isinvolved in the development of cardiac hypertrophy and fibrogenesis of ratsinduced by isoproterenol（ISO）.Methods:Thirty male Wistar rats were randomlydivided into 3 *** 1 was the healthy control group,group 2 was the ISOgroup,and group 3 was the ISO+UII *** groups 2 and 3,ISO(5 mg-kg-1.d-1)wasgiven（sc）once daily for 7 *** 3 was also given UII in the first day [3 nmol/kg（5μg/kg）,iv],followed by sc（1.5 μg/kg）twice *** 1 received 0.9% *** receptor（UT）mRNA expression was determined by *** contents ofUII and angiotensin II（Ang II）were determined by *** vitro,the effects of UII on DNA/collagen synthesis of cardiac fibroblasts were deter-mined by [3H]thymidine/[3H]proline ***:The ratio of heartweight/body weight,plasma lactate dehydrogenase activity,myocardialmalondialdehyde and hydroxyproline concentration increased significantly in theISO group,as well as UT mRNA expression,plasma and cardiac UII and ventricu-lar Ang II,compared with the control group（P0.01）.ISO induced significantmyocardial ***,UII+ISO co-treatment significantly increasedthe changes of biochemical markers of injury and the degree of cardiac hypertro-phy and *** vitro,5×10～9-5×10-7mol/L UII stimulated[3H]thymidine/[3H]proline incorporation into cardiac fibroblasts in a dose-dependent manner（P0.01）.Conclusion:These results suggest that UII was involved in the development ofcardiac fibrosis and hypertrophy by synergistic effects with ISO.
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