Identification and Validation of HYAL1 DNA Methylation in Differentiating Malignant from Benign Thyroid Nodules
作者单位：徐州医科大学附属淮安医院 淮安市第二人民医院
会议名称：《2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛》
会议日期：2021年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Thyroid nodules HYAL1 Identification DNA methylation Protein
摘 要：Background: Thyroid cancer(TC) is the most common endocrine cancer around the world, of which papillary thyroid cancer(PTC) accounts for about 85%. Fine-needle aspiration biopsy(FNAB) is the preferred method for the diagnosis of thyroid nodules in the clinical application. However, about 20-30% of cases are indeterminate nodules according to FNAB, of which approximately 69% are classified as benign by postoperative pathology. Besides, the sensitivities of FNAB are 65-99% across studies. It is thus of great importance to develop novel biomarkers to distinguish the malignant tumors from the benign ones. DNA methylation is commonly dysregulated in a variety of diseases including cancers. Our previous Infinium Methylation EPIC 850 K beadchip assay indicated a differentially methylated CpG site(cg02183083) within hyaluronidase 1(HYAL1) gene. Here, we conducted a validation study to explore the association between HYAL1 and thyroid *** and methods: We measured the HYAL1 methylation level in formalin-fixed paraffin-embedded(FFPE) tissues of 195 early-stage PTC(91.05% PTC at stage Ⅰ) and 195 age-and gender-matched benign tumors collected during 2019-2020. The median(interquartile range, IQR) ages of malignant and benign cases were 51.00(44.00-57.00) and 53.00(46.00-60.25) years old, respectively. Females accounted for 78.95%(150/190) in both PTC and benign cases. Protein expression of HYAL1 was evaluated in another 55 pairs of early-stage PTC(89.09% PTC at stage Ⅰ) and benign tumors collected during 2013-2016, of which 34 pairs of cases were females. The median age was 43.00(31.00-51.00)and 47.00(36.00-52.00) years in PTC and benign *** methylation was quantified by matrixassisted laser desorption ionization time-of-flight mass spectrometry and protein expression was detected by immunohistochemical(IHC) staining. Binary logistic regression and Mann-Whitney U test were used for the data analysis. Two-sided P values 0.05 were considered as statistically