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Two-Staged Method for Ice Channel Identification Based on Image Segmentation and Corner Point Regression

China Ocean Engineering 2024年第2期38卷 313-325页

作者： DONG Wen-bo ZHOU Li DING Shi-feng WANG Ai-ming CAI Jin-yan School of Naval Architecture and Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Identification of the ice channel is the basic technology for developing intelligent ships in ice-covered waters,which is important to ensure the safety and economy of navigation.In the Arctic,merchant ships with low ice class often navigate in channels opened up by icebreakers.Navigation in the ice channel often depends on good maneuverability skills and abundant experience from the captain to a large extent.The ship may get stuck if steered into ice fields off the channel.Under this circumstance,it is very important to study how to identify the boundary lines of ice channels with a reliable method.In this paper,a two-staged ice channel Identification method is developed based on image segmentation and corner point regression.The first stage employs the image segmentation method to extract channel regions.In the second stage,an intelligent corner regression network is proposed to extract the channel boundary lines from the channel region.A non-intelligent angle-based filtering and clustering method is proposed and compared with corner point regression network.The training and evaluation of the segmentation method and corner regression network are carried out on the synthetic and real ice channel dataset.The evaluation results show that the accuracy of the method using the corner point regression network in the second stage is achieved as high as 73.33%on the synthetic ice channel dataset and 70.66%on the real ice channel dataset,and the processing speed can reach up to 14.58frames per second.

关键词： ice channel ship navigation Identification image segmentation corner point regression

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Hyperspectral remote sensing Identification of marine oil emulsions based on the fusion of spatial and spectral features

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Acta Oceanologica Sinica 2024年第3期43卷 139-154页

作者： Xinyue Huang Yi Ma Zongchen Jiang Junfang Yang School of Geography and Marine Science Nanjing UniversityNanjing 210023China First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Marine Telemetry Technology Innovation Center Ministry of Natural ResourcesQingdao 266061China Harbin Institute of Technology Harbin 150001China China University of Petroleum(East China) Qingdao 266061China

Marine oil spill emulsions are difficult to recover,and the damage to the environment is not easy to eliminate.The use of remote sensing to accurately identify oil spill emulsions is highly important for the protection of marine environments.However,the spectrum of oil emulsions changes due to different water content.Hyperspectral remote sensing and deep learning can use spectral and spatial information to identify different types of oil emulsions.Nonetheless,hyperspectral data can also cause information redundancy,reducing classification accuracy and efficiency,and even overfitting in machine learning models.To address these problems,an oil emulsion deep-learning Identification model with spatial-spectral feature fusion is established,and feature bands that can distinguish between crude oil,seawater,water-in-oil emulsion(WO),and oil-in-water emulsion(OW)are filtered based on a standard deviation threshold–mutual information method.Using oil spill airborne hyperspectral data,we conducted Identification experiments on oil emulsions in different background waters and under different spatial and temporal conditions,analyzed the transferability of the model,and explored the effects of feature band selection and spectral resolution on the Identification of oil emulsions.The results show the following.(1)The standard deviation–mutual information feature selection method is able to effectively extract feature bands that can distinguish between WO,OW,oil slick,and seawater.The number of bands was reduced from 224 to 134 after feature selection on the Airborne Visible Infrared Imaging Spectrometer(AVIRIS)data and from 126 to 100 on the S185 data.(2)With feature selection,the overall accuracy and Kappa of the Identification results for the training area are 91.80%and 0.86,respectively,improved by 2.62%and 0.04,and the overall accuracy and Kappa of the Identification results for the migration area are 86.53%and 0.80,respectively,improved by 3.45%and 0.05.(3)The oil emulsion Identification model has a certain degree of trans

关键词： oil emulsions Identification hyperspectral remote sensing feature selection convolutional neural network(CNN) spatial-temporal transferability

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Progress in the preparation,Identification and biological activity of walnut peptides

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未来食品学报(英文) 2024年第3期4卷 205-220页

作者： Chang Liu Zijie Zhang Yuting Shang Siting Li Junxi Xia Yiling Tian Yingmin Jia Aijin Ma School of Food and Health Beijing Technology and Business UniversityBeijing 100048China Hebei Yangyuan ZhiHui Beverage Co. Ltd.Hengshui 053000China Institution of Chinese Walnut Industry Hengshui 053000China Hebei Key Laboratory of Walnut Nutritional Function and Processing Technology Hengshui 053000China College of Food Science and Technology Hebei Agricultural UniversityBaoding 071000China

Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the preparation of functional walnut peptide ingredients or additives and other compositions has received increasing attention.However,the improvement of the yield of walnut peptides and the clarification of their functional activities are the bottlenecks that limit the development of these peptides.To this end,this article reviews the pretreatment,preparation,purification and Identification processes of walnut peptides,as well as their biological activities such as antioxidant activity,antitumour activity,improvement of memory,antihypertension and regulation of metabolic disorders are elaborated to provide a reference for the industrial development of walnut peptides.

关键词： Walnut Peptide Extraction Isolation Purification Identification Biological activity

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Application of Next Generation Sequencing for Rapid Identification of Lactic Acid Bacteria

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Asian Agricultural Research 2024年第4期16卷 27-32页

作者： Xiaxia HOU Yunxia WANG Shuhuan ZHAO Hongbing JIA Cuizhi LI Inner Mongolia Yili Industrial Group Co. Ltd.Hohhot 010110China

The rapid Identification of lactic acid bacteria,which are essential microorganisms in the food industry,is of great significance for industrial applications.The Identification of lactic acid bacteria traditionally relies on the isolation and Identification of pure colonies.While this method is well-established and widely used,it is not without limitations.The subjective judgment inherent in the isolation and purification process introduces potential for error,and the incomplete nature of the isolation process can result in the loss of valuable information.The advent of next generation sequencing has provided a novel approach to the rapid Identification of lactic acid bacteria.This technology offers several advantages,including rapidity,accuracy,high throughput,and low cost.Next generation sequencing represents a significant advancement in the field of DNA sequencing.Its ability to rapidly and accurately identify lactic acid bacteria strains in samples with insufficient information or in the presence of multiple lactic acid bacteria sets it apart as a valuable tool.The application of this technology not only circumvents the potential errors inherent in the traditional method but also provides a robust foundation for the expeditious Identification of lactic acid bacteria strains and the authentication of bacterial powder in industrial applications.This paper commences with an overview of traditional and molecular biology methods for the Identification of lactic acid bacteria.While each method has its own advantages,they are not without limitations in practical application.Subsequently,the paper provides an introduction of the principle,process,advantages,and disadvantages of next generation sequencing,and also details its application in strain Identification and rapid Identification of lactic acid bacteria.The objective of this study is to provide a comprehensive and reliable basis for the rapid Identification of industrial lactic acid bacteria strains and the authenticity Identification of bacterial powder.

关键词： Lactic acid bacteria Rapid Identification Next generation sequencing

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Pharmacognostic Identification of Hedyotis auricularia and Mitracarpus villosus

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Medicinal Plant 2024年第1期15卷 24-27,34页

作者： Piaoling HUANG Feipeng HUANG Xinxin LU Zhonghua DAI Hailin LU Guangxi University of Chinese Medicine Nanning 530001China

[Objectives]To conduct the pharmacognostic Identification of Hedyotis auricularia and Mitracarpus villosus in Guangxi and provide a scientific basis for their Identification.[Methods]The characteristics of original plants were studied by origin Identification method;the properties and characteristics were studied by character Identification method;and the microscopic features of the roots,stems,leaves and medicinal powder of H.auricularia and M.villosus in Guangxi were studied by paraffin method and powder slicing method.[Results](i)Origin Identification.H.auricularia:Leaves leathery,apex acuminate,base cuneate;petiole shorter;cyme axillary;corolla hairy at throat;fruit indehiscent at maturity;testa black after drying.M.villosus:Leaf apex short pointed,base attenuate,blade sessile;flowers small,clustered in axillary;fruits dehiscent by lid at or below middle at maturity,seeds dark brown.(ii)Character Identification.Fracture surface of H.auricularia uneven,white in outer layer and sepia in inner layer.Fracture surface of M.villosus hollow,uneven and white.(iii)Microscopic Identification.H.auricularia:Root phloem thick,cambium visible,duct cells quasi-polygonal,large;rays obvious.Stem transection quasi-circular square,often with non-glandular hairs on epidermis;calcium oxalate raphides present in leaf parenchymal cells.Power grayish brown,starch granules single-grained;calcium oxalate raphides frequent,calcium oxalate clustered crystals occasional;catheter spiral,rarely annular,stomata infinitive.M.villosus:Root parenchyma cells with scattered calcium oxalate raphides,calcium oxalate clustered crystals and brownish red substances visible.Stem transection quasi-square,edge angle with 4 short narrow wings.Powder brown,simple starch granules numerous,compound starch granules also present;calcium oxalate raphides numerous,calcium oxalate clustered crystals and calcium oxalate square cubic crystals also present;catheter spiral,stomata paracytic.[Conclusions]The above transaction microscopic characteristics of the roots,

关键词： Hedyotis auricularia Mitracarpus villosus Pharmacognostic Identification

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Curve Trajectory Model for Human Preferred Path Planning of Automated Vehicles

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Automotive Innovation 2024年第1期7卷 59-70页

作者： Gergo Ferenc Igneczi Erno Horvath Roland Toth Krisztian Nyilas Vehicle Research Center Szechenyi Istvan UniversityEgyetem ter 1Gyor 9026Hungary Institute for Computer Science and Control Kende str.13-17.Budapest 1111Hungary Robert Bosch Kft Gyomroi str.104-120Budapest 1103Hungary

Automated driving systems are often used for lane keeping tasks.By these systems,a local path is planned ahead of the vehicle.However,these paths are often found unnatural by human drivers.In response to this,this paper proposes a linear driver model,which can calculate node points reflective of human driver preferences and based on these node points a human driver preferred motion path can be designed for autonomous driving.The model input is the road curvature,effectively harnessed through a self-developed Euler-curve-based curve fitting algorithm.A comprehensive case study is undertaken to empirically validate the efficacy of the proposed model,demonstrating its capacity to emulate the average behavioral pat-terns observed in human curve path selection.Statistical analyses further underscore the model's robustness,affirming the authenticity of the established relationships.This paradigm shift in trajectory planning holds promising implications for the seamless integration of autonomous driving systems with human driving preferences.

关键词： Naturalistic driving Identification Driver models Path planning

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Identification and semisynthesis of(-)-anisomelic acid as oral agent against SARS-CoV-2 in mice

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National Science Review 2022年第11期9卷 29-36页

作者： Hai-Xin Yu Nan Zheng Chi-Tai Yeh Chien-Ming Lee Qi Zhang Wen-Lv Zheng Qing Chang Yuan-He Li Yu-Jun Li Gui-Zhen Wu Jun-Min Quan Lin-Qi Zhang Yew-Min Tzeng Zhen Yang Laboratory of Chemical Genomics School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School Department of Medicinal Research and Education Taipei Medical University-Shuang Ho Hospital Department of Applied Science Taitung University Center for Global Health and Infectious Diseases Comprehensive AIDS Research Center and Beijing Advanced Innovation Center for Structural Biology School of Medicine Tsinghua University Lanzhou Institute of Separation Science State Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education and Beijing National Laboratory for Molecular Science(BNLMS) College of Chemistry and Molecular EngineeringPeking University Shenzhen Bay Laboratory NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Applied Chemistry Chaoyang University of Technology

(-)-Anisomelic acid, isolated from Anisomeles indica(L.) Kuntze(Labiatae) leaves, is a macrocyclic cembranolide with a trans-fused α-methylene-γ-lactone motif. Anisomelic acid effectively inhibits SARS-Co V-2 replication and viral-induced cytopathic effects with an EC50 of 1.1 and 4.3 μM, respectively.Challenge studies of SARS-CoV-2-infected K18-hACE2 mice showed that oral administration of anisomelic acid and subcutaneous dosing of remdesivir can both reduce the viral titers in the lung tissue at the same level. To facilitate drug discovery, we used a semisynthetic approach to shorten the project timelines. The enantioselective semisynthesis of anisomelic acid from the naturally enriched and commercially available starting material(+)-costunolide was achieved in five steps with a 27% overall yield.The developed chemistry provides opportunities for developing anisomelic-acid-based novel ligands for selectively targeting proteins involved in viral infections.

关键词： Identification enantioselective semisynthesis (−)-anisomelic acid oral agent anti-SARS-CoV-2

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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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Food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er Tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

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Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development of Marsupenaeus japonicas

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Journal of Oceanology and Limnology 2024年第1期42卷 201-215页

作者： Jing WANG Longjun PU Xiaojuan ZHANG Cuicui LIANG Dandan DONG Jiantao GUAN Huarong GUO Ministry of Education Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

Regulation of microRNAs(miRNAs)on various biological processes has been a surprising and exciting field.Identification of miRNAs is the first step to comprehensively understand their functions.However,attempts on global Identification and functional verification of miRNAs are very limited in penaeid shrimp Marsupenaeus japonicus,an economically important aquatic species.By performing an integrated analysis of transcriptomic profile from gastrula embryos of M.japonicus,21conserved miRNAs in M.japonicas(mja-miRNAs),belonging to 19 miRNA families,were identified and characterized.Of the 21 mja-miRNAs,15 miRNAs were successfully verified to be predominantly expressed in gastrula stage,where they displayed dynamic expression patterns compared with those in naupliuin stage.Based on perfect or near-perfect match to target region,120 target genes were predicted at transcriptome-wide level.Noteworthy,gene ontology(GO)classification and metabolic pathway annotation revealed eight targets that were actively involved in developmental processes.Of the predicted miRNA-mRNA pairs,six targets were then randomly selected and experimentally validated by dual luciferase reporter assay,where three pairs were proved with potential targeting activity.Overall,to search for conserved miRNAs potentially involved in early development of M.japonicus,we combined in silico and experimental methods,which can be applied in other organisms as well.Our data implied important roles of miRNAs in the early embryonic development and also suggested the presence of complex miRNA-mRNA functional networks in M.japonicus.

关键词： microRNAs(miRNAs) Identification Marsupenaeus japonicus embryo transcriptome

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Identification and characterization of Fe_(3)O_(4)/peroxodisulfate advanced oxidation products from sulfameter

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Journal of Environmental Sciences 2022年第12期34卷 227-235页

作者： Jie Du Zhijun An Yufeng Hu Xianliang Yi Hao Zhou Jingjing Zhan Minghuo Wu School of Ocean Science and Technology Dalian University of TechnologyPanjin 124221China

Sulfonamides(SAs)are one of the most widely used antibiotics and their residuals in the environment could cause some negative environmental issues.Advanced oxidation such as Fenton-like reaction has been widely applied in the treatment of SAs polluted water.Degradation rates of 95%-99.7%were achieved in this work for the tested 8 SAs,including sulfisomidine,sulfameter(SME),phthalylsulfathiazole,sulfamethoxypyridazine,sulfamonomethoxine,sulfisoxazole,sulfachloropyridazine,and sulfadimethoxine,in the Fe_(3)O_(4)/peroxodisulfate(PDS)oxidation system after the optimization of PDS concentration and p H.Meanwhile,it was found that a lot of unknown oxidation products were formed,which brought up the uncertainty of health risks to the environment,and the Identification of these unknown products was critical.Therefore,SME was selected as the model compound,from which the oxidation products were never elucidated,to identify these intermediates/products.With liquid chromatography-high resolution tandem mass spectrometry(LC-HRMS/MS),10 new products were identified,in which 2-amino-5-methoxypyrimidine(AMP)was confirmed by its standard.The investigation of the oxidation process of SME indicated that most of the products were not stable and the degradation pathways were very complicated as multiple reactions,such as oxidation of the amino group,SO_(2)extrusion,and potential cross-reaction occurred simultaneously.Though most of the products were not verified due to the lack of standards,our results could be helpful in the evaluation of the treatment performance of SAs containing wastewater.

关键词： Advanced oxidation Sulfonamides LC-HRMS/MS Identification Degradation products

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