Molecular detection of virulence genes in Campylobacter species isolated from livestock production systems in South Africa

作     者：Bongekile NGOBESE Oliver Tendayi ZISHIRI Mohamed Ezzat EL ZOWALATY Bongekile NGOBESE;Oliver Tendayi ZISHIRI;Mohamed Ezzat EL ZOWALATY

作者机构：Discipline of GeneticsSchool of Life SciencesUniversity of KwaZulu-NatalDurban 4000South Africa Zoonosis Science CenterDepartment of Medical Biochemistry and MicrobiologyUppsala UniversityUppsala SE-75185Sweden Infectious Diseases and Anti-Infective Therapy Research GroupCollege of Pharmacy and Sharjah Medical Research InstituteUniversity of SharjahSharjah 27272UAE 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第6期

页      面：1656-1670页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 090601[农学-基础兽医学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：We would like to thank the South African National Research Foundation for supporting this research through the Thuthuka Funding Instrument(TTK170411226583).We would also like to thank the College of Agriculture Engineering and Science as well as the School of Life Sciences at University of KwaZulu-Natal(Westville Campus) South Africa for their support during the execution of this research.Authors would like to thank the anonymous reviewers for their valuable comments that significantly improved the manuscript 

主　　题：Campylobacter zoonotic cytotoxicity pathogenicity virulenee campylobacteriosis foodbome livestock PCR one-health 

摘      要：Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries *** jejuni is responsible for the majority of infectio *** study was con ducted to identify virule nceassociated genes in Campylobacter species isolated from livestock production systems in South Africa.A total of 250 fecal samples consisting of cattle(n=50),chickens(n=50),goats(n=50),sheep(n=50)and pigs(n=50)were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October *** samples were an a lyzed for the presence of virule nee genes in Campylobacter species using molecular PCR-based *** was found that 77 and 23%of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock *** were positive significant(P0.05)correlations amongst all the virulenee genes that were *** and Fisher s exact tests were implemented to test for the effect of livestock species on the presenee or absenee of virule nee gen *** study dem on strated that most of livestock species can pote ntially cause zoonotic infecti on s and food pois oning due to the high prevale nee of *** high prevale nee of virule nee genes highlights the sign ifica nee of Campylobacter\r\livestock production systems in South *** requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.