C60 Fullerenes Suppress Reactive Oxygen Species Toxicity Damage in Boar Sperm

作     者：Xinhong Li Lirui Wang Huan Liu Jieli Fu Linqing Zhen Yuhua Li Yaozhong Zhang Yafei Zhang 

作者机构：Shanghai Key Laboratory of Veterinary BiotechnologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Institute of Nano Biomedicine and EngineeringShanghai Engineering Research Centre for Intelligent Diagnosis and Treatment InstrumentDepartment of Instrument Science and EngineeringSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Department of Electrical and Computer EngineeringMichigan State UniversityEast LansingUSA Key Laboratory of Thin Film and Microfabrication(Ministry of Education)Department of Micro/Nano ElectronicsSchool of ElectronicsInformation and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2019年第11卷第4期

页      面：817-833页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 0905[农学-畜牧学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0703[理学-化学] 090501[农学-动物遗传育种与繁殖] 070301[理学-无机化学] 

基　　金：supported by the National Natural Science Foundation of China(No.31772594) the Special Fund for Agro-scientific Research in the Public Interest of China(No.200903056) the Key Project of Shanghai Municipal Agricultural Commission of China(2014-2-5) 

主　　题：Carboxylated C60 Semen preservation Oxidative stress Motility Protein dephosphorylation 

摘      要：We report the carboxylated C60 improved the survival and quality of boar sperm during liquid storage at 4°C and thus propose the use of carboxylated C60 as a novel antioxidant semen extender *** results demonstrated that the sperm treated with 2μg mL?1 carboxylated C60 had higher motility than the control group(58.6%and 35.4%,respectively;P?0.05).Moreover,after incubation with carboxylated C60 for 10 days,acrosome integrity and mitochondrial activity of sperm increased by 18.1%and 34%,respectively,compared with that in the control ***,the antioxidation abilities and adenosine triphosphate levels in boar sperm treated with carboxylated C60 significantly increased(P?0.05)compared with those in the control *** presence of carboxylated C60 in semen extender increases sperm motility probably by suppressing reactive oxygen species(ROS)toxicity ***,carboxylated C60 could protect boar sperm from oxidative stress and energy deficiency by inhibiting the ROS-induced protein dephosphorylation via the cAMP-PKA signaling *** addition,the safety of carboxylated C60 as an alternative antioxidant was also comprehensively evaluated by assessing the mean litter size and number of live offspring in the carboxylated C60 treatment *** findings confirm carboxylated C60 as a novel antioxidant agent and suggest its use as a semen extender supplement for assisted reproductive technology in domestic animals.