Dynamic changes of behaviors, dentate gyrus neurogenesis and hippocampal miR-124 expression in rats with depression induced by chronic unpredictable mild stress

作     者：Yun-Ling Huang Ning-Xi Zeng Jie Chen Jie Niu Wu-Long Luo Ping Liu Can Yan Li-Li Wu Yun-Ling Huang;Ning-Xi Zeng;Jie Chen;Jie Niu;Wu-Long Luo;Ping Liu;Can Yan;Li-Li Wu

作者机构：Research Center for Basic Integrative MedicineGuangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Department of PharmacologyPLA General HospitalBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第6期

页      面：1150-1159页

核心收录：

学科分类：10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81573858(to LLW) the Natural Science Foundation of Guangdong Province of China,No.2016A030313648(to CY) the Major Basic Research Project of Educational Commission of Guangdong Province of China,No.2017KZDXM020(to CY) 

主　　题：chronic unpredictable mild stress model continuous observation depression depression-like behavior dynamic changes hippocampus miR-124 neurogenesis dysfunction neuronal loss 

摘      要：The depression-like behavior phenotype,neurogenesis in the dentate gyrus and miR-124 expression in the hippocampus are the focus of current research on the pathogenesis of depression and antidepressant *** present study aimed to clarify the dynamic changes of depression-like behavior,dentate gyrus neurogenesis and hippocampal miR-124 expression during depression induced by chronic stress to reveal pathological features at different stages of depression and to further provide insight into depression *** unpredictable mild stress depression models were established by exposing Sprague-Dawley rats to various mild stressors,including white noise,thermal swimming,stroboscopic illumination,soiled cages,pairing with three other stressed animals,cold swimming,tail pinch,restraint and water and food *** unpredictable mild stress model rats underwent dynamic observation from 1 to 8 weeks and were compared with a control group(normal feeding without any stressors).To observe changes in the depression-like behavior phenotype during chronic unpredictable mild stress-induced depression,a sucrose preference test was used to evaluate the degree of *** open-field test was used to evaluate locomotor activity and anxiety *** with the control group,chronic unpredictable mild stress rats lost weight but did not have a depression-like behavioral phenotype at 1-4 *** unpredictable mild stress rats presented decreased sucrose preference and locomotor activity at 5-8 *** addition,chronic unpredictable mild stress rats did not have significant anxiety-like behavior during 1-8 weeks of *** observe neurogenesis dysfunctions and changes in neuronal number in the dentate gyrus during chronic unpredictable mild stress-induced depression,markers(DCX and DCX/BrdU)of neural proliferation and differentiation and the neuronal marker NeuN were assessed by *** with the control group,neurogenesis and