Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs

作     者：Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou Rui Fu;Dawei Yu;Jilong Ren;Chongyang Li;Jing Wang;Guihai Feng;Xuepeng Wang;Haifeng Wan;Tianda Li;Libin Wang;Ying Zhang;Tang Hai;Wei Li;Qi Zhou

作者机构：State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and RegenerationChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2020年第11卷第2期

页      面：97-107页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16030400 to W.L.) the National Key Research and Development Program(2016YFA0100202 and 2017YFA0104401 to T.H.,2018YFA0109701 to R.F.) the National Natural Science Foundation of China(31621004 to Q.Z.and W.L.,31571533 to H.W.,and 31701286 to G.F.) the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences(QYZDY-SSWSMC002 to Q.Z.) 

主　　题：embryonic stem cells,blastocyst complementation cynomolgus monkey pig interspecies chimera organ reconstruction 

摘      要：Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic ***,ethical issues prevent the study of human chimeras in the late embryonic stage of *** embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ ***,whether primate ESCs can be used in xenogenous grafts remains *** this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to *** report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ *** addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC *** together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate *** believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation.