检索结果-南通市图书馆

The monkey microbial biobank brings previously uncultivated bioresources for nonhuman primate and human gut microbiomes

引用

mLife 2022年第2期1卷 210-217页

作者： Danhua Li Chang Liu Rexiding Abuduaini Mengxuan Du Yujing Wang Haizhen Zhu Honghe Chen Nan Zhou Yuhua Xin Linhuan Wu Juncai Ma Yuguang Zhou Yong Lu Chengying Jiang Qiang Sun Shuang-Jiang Liu State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center at Institute of Microbiology Chinese Academy of SciencesBeijingChina College of Life Science University of Chinese Academy of SciencesBeijingChina State Key Laboratory of Microbial Biotechnology Shandong UniversityQingdaoChina China General Microorganism Culture Collection Institute of MicrobiologyChinese Academy of SciencesBeijingChina Microbial Resources and Big Data Center Institute of MicrobiologyChinese Academy of SciencesBeijingChina State Key Laboratory of Neuroscience CAS Key Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesInstitute of NeuroscienceShanghaiChina

Impact statement Nonhuman primates(NHPs)such as monkeys are the closest living relatives to humans and are the best available models for causative studies of human health and diseases.Gut microbiomes are intensively involved in host health.In this study,by large-scale cultivation of microbes from fecal samples of monkeys,we obtained previously uncultured bacterial species and constructed a Macaca fascicularis Gut Microbial Biobank(MfGMB).The MfGMB consisted of 250 strains that represent 97 species of 63 genera,25 families,and 4 phyla.The information of the 250 strains and the genomes of 97 cultured species are publicly accessible.The MfGMB represented nearly 50% of core gut microbial compositions at the genus level and covered over 80% of the KO-based known gut microbiome functions of M.fascicularis.Data mining showed that the bacterial species in the MfGMB were prevalent not only in NHPs gut microbiomes but also in human gut microbiomes.This study will help the understanding and future investigations on how gut microbiomes interact with their mammalian hosts.

关键词： statement monkey gut

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Co-editing PINK1 and DJ-1 Genes Via Adeno-Associated Virus-Delivered CRISPR/Cas9 System in Adult monkey Brain Elicits Classical Parkinsonian Phenotype

引用

Neuroscience Bulletin 2021年第9期37卷 1271-1288页

作者： Hao Li Shihao Wu Xia Ma Xiao Li Tianlin Cheng Zhifang Chen Jing Wu Longbao Lv Ling Li Liqi Xu Wenchao Wang Yingzhou Hu Haisong Jiang Yong Yin Zilong Qiu Xintian Hu Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of Sciences650223 KunmingChina Institute of Neuroscience CAS Key Laboratory of Primate NeurobiologyState Key Laboratory of NeuroscienceChinese Academy of SciencesShanghai 200031China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China National Resource Center for Non-human Primates Kunming Primate Research Centerand National Research Facility for Phenotypic and Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of Sciences650107 KunmingChina Diagnostic Radiology Department 920 Hospital of the Joint Logistics Support Force of the PLAKunming 650032China Ultrasound diagnosis Department 920 Hospital of the Joint Logistics Support Force of the PLAKunming 650032China Sichuan Provincial People’s Hospital University of Electronic Science and Technology of ChinaChengdu 610072China Department of Rehabilitation Medicine The Second People’s Hospital of Yunnan ProvinceKunming 650021China National Clinical Research Center for Aging and Medicine Huashan HospitalFudan UniversityShanghai 200433China

Whether direct manipulation of Parkinson’s disease(PD)risk genes in the adult monkey brain can elicit a Parkinsonian phenotype remains an unsolved issue.Here,we used an adeno-associated virus serotype 9(AAV9)-delivered CRISPR/Cas9 system to directly co-edit PINK1 and DJ-1 genes in the substantia nigras(SNs)of two monkey groups:an old group and a middle-aged group.After the operation,the old group exhibited all the classic PD symptoms,including bradykinesia,tremor,and postural instability,accompanied by key pathological hallmarks of PD,such as severe nigral dopaminergic neuron loss(>64%)and evidentα-synuclein pathology in the gene-edited SN.In contrast,the phenotype of their middle-aged counterparts,which also showed clear PD symptoms and pathological hallmarks,were less severe.In addition to the higher final total PD scores and more severe pathological changes,the old group were also more susceptible to gene editing by showing a faster process of PD progression.These results suggested that both genetic and aging factors played important roles in the development of PD in the monkeys.Taken together,this system can effectively develop a large number of genetically-edited PD monkeys in a short time(6–10 months),and thus provides a practical transgenic monkey model for future PD studies.

关键词： Parkinson’s disease monkey Adeno-associated virus-delivered CRISPR/Cas9 PINK1 DJ-1 Parkinsonian phenotype

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs

引用

Protein & Cell 2020年第2期11卷 97-107页

作者： Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic organs.However,ethical issues prevent the study of human chimeras in the late embryonic stage of development.Primate embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ generation.However,whether primate ESCs can be used in xenogenous grafts remains unclear.In this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to humans.We report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ layers.In addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC differentiation.Taken together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate tissues.We believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation.

关键词： embryonic stem cells,blastocyst complementation cynomolgus monkey pig interspecies chimera organ reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

monkey

引用

满族文学 2013年第3期 12-18页

作者：陈再见

后来我一直想,一个人的长相如果太独特的话,是不是就决定他会和别人不一样?或者,会有一个出乎意料的结局?他就像只猴子,他其实也挺高,又瘦,就像那种长臂的可以在树林间荡来荡去的猴子.我当初的第一印象是这样.那时我刚上初中一年级,对... 详细信息

后来我一直想,一个人的长相如果太独特的话,是不是就决定他会和别人不一样?或者,会有一个出乎意料的结局?他就像只猴子,他其实也挺高,又瘦,就像那种长臂的可以在树林间荡来荡去的猴子.我当初的第一印象是这样.那时我刚上初中一年级,对别的课本没啥兴趣,那本红色封面的英语却翻了又翻,老师新教的一个词,monkey,猴子的意思.我竟有恍然大悟之感,像是一个词终于找到了它的用处,他不就是猴子嘛,他正好就是monkey.于是,他再次出现在我家时,我就唤他monkey了,而他只能朝我笑,他根本不知道我在说他什么.我的乐趣正源于此.

关键词： monkey

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Establishment of porcine and monkey colonic organoids for drug toxicity study

引用

Cell Regeneration 2021年第1期10卷 342-351页

作者： Haonan Li Yalong Wang Mengxian Zhang Hong Wang Along Cui Jianguo Zhao Weizhi Ji Ye-Guang Chen The State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China State Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunming 650500YunnanChina School of Life Sciences University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine Guangzhou Regenerative Medicine and Health Guangdong LaboratoryGuangzhou 510700China

Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity,but high cost limits their uses.Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions.However,colonic organoids of pig and monkey are not yet established.Here,we report a culture medium to support the growth of porcine and monkey colonic organoids.Wnt signaling and PGE2 are important for long-term expansion of the organoids,and their withdrawal results in lineage differentiation to mature cells.Furthermore,we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response.Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies.

关键词： Pig monkey Colonic organoids Culture Drug toxicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey （Macaca mulatta）

引用

Asian Journal of Andrology 2015年第6期17卷 972-980,I0009页

作者： Mahmoud Huleihel Seyedmehdi Nourashrafeddin Tony M Plant The Shraga Segal Department of Microbiology Immunology and Genetics Faculty of Health Sciences Ben-Gurion University of the Negev Beer-Sheva Israel Department of Obsteirics Gynecology and Reproductive Sciences Univeisity of Pittsburgh School of Medicine Magee-Womens Research InstitutePittsburgh PA 15213 USA

In vitro culture of spermatogonial stem cells （SSCs） has generally been performed using two-dimensional （2D） culture systems; however, such cultures have not led to the development of complete spermatogenesis. It seems that 2D systems do not replicate optimal conditions of the seminiferous tubules （including those generated by the SSC niche） and necessary for spermatogenesis. Recently, one of our laboratories has been able to induce proliferation and differentiation of mouse testicular germ cells to meiotic and postmeiotic stages including generation of sperm in a 3D soft agar culture system （SACS） and a 3D methylcellulose culture system （MCS）. It was suggested that SACS and MCS form a special 3D microenvironment that mimics germ cell niche formation in the seminiferous tubules, and thus permits mouse spermatogenesis in vitro. In this review, we （1） provide a brief overview of the differences in spermatogenesis in rodents and primates, （2） summarize data related to attempts to generate sperm in vitro, （3） report for the first time formation of colonies/clusters of cells and differentiation of meiotic （expression of CREM-1） and postmeiotic （expression of acrosin） germ cells from undifferentiated spermatogonia isolated from the testis of prepubertal rhesus monkeys and cultured in SACS and MCS, and （4） indicate research needed to optimize 3D systems for in vitro primate spermatogenesis and for possible future application to man.

关键词： in vitrospermatogenesis methylcellulose culture system monkey primates soft agar culture system three-dimensional culture system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Generation of a monkey with MECP2 mutations by TALEN-based gene targeting

引用

Neuroscience Bulletin 2014年第3期30卷 381-386页

作者： Zhen Liu Xue Zhou Ying Zhu Zhi-Fang Chen Bin Yu Yan Wang Chen-Chen Zhang Yan-Hong Nie Xiao Sang Yi-Jun Cai Yue-Fang Zhang Chen Zhang Wen-Hao Zhou Qiang Sun Zilong Qiu Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Neonatology Children’s Hospital of Fudan University State Key Laboratory of Biomembrane and Membrane Biotechnology School of Life Sciences PKU-IDG/McGovern Institute for Brain Research Peking University

Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey （Macaca fascicularis） carrying MECP2 mutations using transcription activator-like effector nucleases （TALENs）-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frame- shifting mutation in various tissues. The monkey carrying the MECP2 mutation failed to survive after birth, due to either the toxicity of TALENs or the critical requirement of MECP2 for neural development. The level of MeCP2 protein was essentially depleted in the monkey＇s brain. This study demonstrates the feasibility of introducing genetic mutations in non-human primates by site-specific gene-editing methods.

关键词： monkey TALEN MeCP2 Rett syndrome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ultra-high-performance liquid chromatography for the determination of exenatide in monkey plasma by tandem quadrupole mass spectrometry

引用

Journal of Pharmaceutical Analysis 2013年第4期3卷 235-240页

作者： Jin-Feng Zhang Chun-Jie Sha Yu Sun Yun-Yun Gai Ji-Ye Sun Jiang-Bin Han Xin Shao Chun-Na Sha You-Xin Li Wan-Hui Liu School of Pharmacy Yantai University State Key Laboratory of Long-acting and Targeting Drug Delivery System Shandong Luye Pharmaceutical Co.Ltd. State Key Laboratory of Long-acting and Targeting Drug Delivery System Shandong Luye Pharmaceutical Co. Ltd. Rushan People's Hospital

A highly sensitive ultra-high-performance liquid chromatographic-tandem mass spectro- metry （UPLC/MS/MS） method was developed for the quantification of the synthetic peptide drug of exenatide in monkey plasma. Sample preparation was carried out by solid-phase extraction （SPE）, and bivalirudin was used as the internal standard （IS）. An excellent chromatographic separation was obtained on a reversed-phase C1s column with a gradient elution. Detection utilized a Qtrap 5500 system operated in the positive ion mode with multiple reaction monitoring （MRM）. The proposed method was validated by assessing the specificity, linearity, intra- and inter-day precision and accuracy, recovery, and stability. The method resulted in a linear calibration range of 0.10-30 ng/mL, extracting with only 50 μL monkey plasma aliquots. The intra- and inter-day precisions （as relative standard deviation） were less than 7.5% and 9.6%, respectively. The methodcould be successfully utilized for the pharrnacokinetic study of exenatide in monkeys following a single subcutaneous injection of Byetta.

关键词： Exenatide UPLC/MS/MS Pharmacokineties Plasma monkey

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

monkey Embryos Grown in Dishes Open a New Window on Human Embryonic Development

引用

Bulletin of the Chinese Academy of Sciences 2019年第3期33卷 150-152页

作者： YAN Fusheng 不详

"It is not birth,marriage,or death,but gastrulation,which is truly the most important time in your life,"said Lewis Wolpert in 1986,an eminent developmental biologist.Because during gastrulation,cell migrations lead to a massive reorganization of the embryo from a simple spherical ball of cells,the blastula,into a multi-layered organism.A dearth of primate embryo samples at the gastrulation stage has,however,limited our understanding of this critical event in primates.Now,a newly established platform to grow monkey embryos in dishes could be the ice-breaker.

关键词： monkey New Window

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于monkey和DroidBot的Android应用自动化测试设计

基于Monkey和DroidBot的Android应用自动化测试设计

引用

作者：胡越苏州大学

学位级别：硕士

近年来,随着移动互联网的快速发展,Android系统已经成为了移动设备上最流行的操作系统,同时各大Android应用市场上,新开发的应用数量也呈爆发式增长。随之而来的是Android应用的各种Bug的激增,如:应用对于不同Android版本的兼容性问题... 详细信息

近年来,随着移动互联网的快速发展,Android系统已经成为了移动设备上最流行的操作系统,同时各大Android应用市场上,新开发的应用数量也呈爆发式增长。随之而来的是Android应用的各种Bug的激增,如:应用对于不同Android版本的兼容性问题、恶意扣费、隐私泄露风险等等,不仅给Android用户的系统体验带来了不适,还可能引发用户对于Android设备厂商的各类投诉。如何在Android设备出货前,对Android预装系统进行应用兼容性和安全性评估,提前预知使用者可能遇到的各类问题,并以此改善用户体验,正成为各大Android设备厂商研究热门方向。目前Android设备厂商使用的主流应用自动化测试工具主要基于monkey框架,monkey框架是Android SDK自带的测试工具,在测试过程中会向系统发送伪随机用户事件流,由于测试事件和数据都是随机的,不能自定义,因此有很大的局限。本文以monkey和DroidBot框架为基础,通过自主的二次开发构建了新的自动化测试框架,并且通过实验论证在代码覆盖度和运行效率上均有显著的提升。综上所述,本文研究设计的自动化测试工具已证明可以在实际工作中部署和运行,并且有效地捕获各类Android应用Bug及其Debug log,从而一方面降低了测试人工成本,另一方面也保障了Android设备的软件品质。

关键词： Android monkey DroidBot 自动化测试

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：