Wulong Xiaozheng Wan medicated serum inhibits epithelial-mesenchymal transition in human gastric carcinoma cell line BGC823 by modulation of transforming growth factor-β1/Smad signaling

作     者：Zhang Yali Wang Bingyu Guo Xueying Yang Lei Li Dandan Yuan Xingxing 

作者机构：Department of Gastroenterology Nangang branch of Heilongjiang Academy of Traditional Chinese Medicine Harbin 150006 China Pharmacological Laboratory of Heilongjiang Academy of Traditional Chinese Medicine Harbin 150036China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2019年第39卷第3期

页      面：380-392页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by State Administration of Traditional Chinese Medicine Science and Technology Research Projects(Chinese pharmaceutical 2016ZX05) Effect of Wulong Xiaozheng Wan on Intermediate Gastric Carcinoma and its Mechanism 

主　　题：Transforming growth factor beta1 Smad proteins Signal transduction Epithelial-mesenchymal transition Matrix metalloproteinases,secreted BGC823 cell Wulong Xiaozheng Wan 

摘      要：OBJECTIVE:To evaluate the effect of Wulong Xiaozheng Wan medicated serum on the epithelial-mesenchymal transition (EMT) of BGC823 cell induced by transforming growth factor-β,(TGF-β,) and to explore its ***:EMT model of BGC823 was stimulated by TGF-β*** Xiaozheng Wan medicated serum and LY-364947 were used as *** proliferation and adhesion of BGC823 were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry was used to detect the *** invasion and migration were detected by *** level of matrix metalloproteins was detected by enzyme-linked immunosorbent *** expressions of related proteins and mRNA of EMT marker and TGF-β1/Smad signal pathway were detected by Western blot and reverse transcription-polymerase chain ***:Compared with the TGF-β1 group,Wulong Xiaozheng Wan medicated serum could inhibit the ability of proliferation,heterogeneous adhesion,invasion,and *** also promotes apoptosis and homotypic adhesion in BGC823,with a dose-dependent ***,Wulong Xiaozheng Wan medicated serum could regulate the expression of related proteins and mRNA of TGF-β1/Smad signaling pathway,and inhibit the expressions of EMT transcription factors and EMT ***:Wulong Xiaozheng Wan medicated serum inhibited epithelial-mesenchymal transition by down-regulated the expression of TβRI and the activation of TGF-β1/Smad signaling pathway.