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Cellulose nanofibril Matrix drives the dynamic formation of spheroids

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第10期24卷 922-934页

作者： Yi LU Guo LI Yeqiu LI Yuan YAO College of Chemical and Biological Engineering Zhejiang UniversityHangzhou 310027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311215China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

Multicellular spheroids,which mimic the natural organ counterparts,allow the prospect of drug screening and regenerative medicine.However,their application is hampered by low processing efficiency or limited scale.This study introduces an efficient method to drive rapid multicellular spheroid formation by a cellulose nanofibril Matrix.This Matrix enables the facilitated growth of spheroids(within 48 h)through multiple cell assembly into size-controllable aggregates with well-organized physiological microstructure.The efficiency,dimension,and conformation of the as-formed spheroids depend on the concentration of extracellular nanofibrils,the number of assembled cells,and the heterogeneity of cell types.The above strategy allows the robust formation mechanism of compacted tumoroids and hepatocyte spheroids.

关键词： Cellulose Nanofibril Matrix Self-assembly Spheroid

Aberrant NF-κB activation in odontoblasts orchestrates inflammatory Matrix degradation and mineral resorption

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International Journal of Oral Science 2022年第1期14卷 80-90页

作者： Fanyuan Yu Fengli Huo Feifei Li Yanqin Zuo Chenglin Wang Ling Ye State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengduChina Department of Endodontics West China Stomatology HospitalSichuan UniversityChengduChina

Inflammation-associated proteinase functions are key determinants of inflammatory stromal tissues deconstruction.As a specialized inflammatory pathological process,dental internal resorption(IR)includes both soft and hard tissues deconstruction within the dentin-pulp complex,which has been one of the main reasons for inflammatory tooth loss.Mechanisms of inflammatory Matrix degradation and tissue resorption in IR are largely unclear.In this study,we used a combination of Cre-loxP reporter,flow cytometry,cell transplantation,and enzyme activities assay to mechanistically investigate the role of regenerative cells,odontoblasts(ODs),in inflammatory mineral resorption and matrices degradation.We report that inflamed ODs have strong capabilities of Matrix degradation and tissue resorption.Traditionally,ODs are regarded as hard-tissue regenerative cells;however,our data unexpectedly present ODs as a crucial population that participates in IR-associated tissue deconstruction.Specifically,we uncovered that nuclear factor-kappa b(NF-κB)signaling orchestrated Tumor necrosis factorα(TNF-α)-induced Matrix metalloproteinases(Mmps)and Cathepsin K(Ctsk)functions in ODs to enhance Matrix degradation and tissue resorption.Furthermore,TNF-αincreases Rankl/Opg ratio in ODs via NF-κB signaling by impairing Opg expression but increasing Rankl level,which utterly makes ODs cell line 17IIA11(A11)become Trap^(+) and Ctsk^(+) multinucleated cells to perform resorptive actions.Blocking of NF-κB signaling significantly rescues Matrix degradation and resorptive functions of inflamed ODs via repressing vital inflammatory proteinases Mmps and Ctsk.Utterly,via utilizing NF-κB specific small molecule inhibitors we satisfactorily attenuated inflammatory ODs-associated human dental IR in vivo.Our data reveal the underlying mechanisms of inflammatory Matrix degradation and resorption via proteinase activities in IR-related pathological conditions.

关键词： inflammatory Matrix utilizing

Towards sparse Matrix operations:graph database approach for power grid computation

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Global Energy Interconnection 2023年第1期6卷 50-63页

作者： Daoxing Li Kai Xiao Xiaohui Wang Pengtian Guo Yong Chen China Electric Power Research Institute Co.Ltd. Beijing 100192P.R.China

The construction of new power systems presents higher requirements for the Power Internet of Things(PIoT)technology.The“source-grid-load-storage”architecture of a new power system requires PIoT to have a stronger multi-source heterogeneous data fusion ability.Native graph databases have great advantages in dealing with multi-source heterogeneous data,which make them suitable for an increasing number of analytical computing tasks.However,only few existing graph database products have native support for Matrix operation-related interfaces or functions,resulting in low efficiency when handling Matrix calculations that are commonly encountered in power grids.In this paper,the Matrix computation process is expressed by a strategy called graph description,which relies on the natural connection between the Matrix and structure of the graph.Based on that,we implement Matrix operations on graph database,including Matrix multiplication,Matrix decomposition,etc.Specifically,only the nodes relevant to the computation and their neighbors are concerned in the process,which prunes the influence of zero elements in the Matrix and avoids useless iterations compared to the conventional Matrix computation.Based on the graph description,a series of power grid computations can be implemented on graph database,which reduces redundant data import and export operations while leveraging the parallel computing capability of graph database.It promotes the efficiency of PIoT when handling multi-source heterogeneous data.An comprehensive experimental study over two different scale power system datasets compares the proposed method with Python and MATLAB baselines.The results reveal the superior performance of our proposed method in both power flow and N-1 contingency computations.

关键词： Graph database Graph description Matrix Parallel computing Power flow

Particle manipulation behind a turbid medium based on the intensity transmission Matrix

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Photonics Research 2022年第10期10卷 2293-2301页

作者： KAIGE LIU HENGKANG ZHANG SHANSHAN DU ZEQI LIU BIN ZHANG XING FU QIANG LIU Key Laboratory of Photonics Control Technology Ministry of EducationTsinghua UniversityBeijing 100084China Department of Precision Instrument State Key Laboratory of Precision Measurement Technology and InstrumentsTsinghua UniversityBeijing 100084China Beijing Institute of Control Engineering Beijing 100190China Beijing Institute of Electronic System Engineering Beijing 100854China

Although optical tweezers can manipulate tiny particles,the distortion caused by the scattering medium restricts their application.Wavefront-shaping techniques such as the transmission Matrix(TM)method are powerful tools to achieve light focusing behind the scattering medium.In this paper,we propose a method to focus light through a scattering medium in a large area based on the intensity transmission Matrix(ITM).Only relying on the intensity distribution,we can calculate the ITM with the number of measurements equal to that of the control segments.Free of the diffraction limit,our method guarantees high energy usage of the light field.Based on this method,we have implemented particle manipulation with a high degree of freedom on single and multiple particles.In addition,the manipulation range is enlarged more than 20 times(compared to the memory effect)to 200μm.

关键词： scattering Matrix intensity

Speckle spatial correlations aiding optical transmission Matrix retrieval:the smoothed Gerchberg–Saxton single-iteration algorithm

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Photonics Research 2022年第10期10卷 2349-2358页

作者： DANIELE ANCORA LORENZO DOMINICI ANTONIO GIANFRATE PAOLO CAZZATO MILENA DE GIORGI DARIO BALLARINI DANIELE SANVITTO LUCA LEUZZI Department of Physics Universitàdi Roma la SapienzaPiazzale Aldo Moro 5I-00185 RomeItaly Institute of Nanotechnology Consiglio Nazionale delle Ricerche(CNR-NANOTEC)Via MonteroniI-73100 LecceItaly Institute of Nanotechnology Soft and Living Matter LaboratoryConsiglio Nazionale delle Ricerche(CNR-NANOTEC)Piazzale Aldo Moro 5I-00185 RomeItaly

The estimation of the transmission Matrix of a disordered medium is a challenging problem in disordered photonics.Usually,its reconstruction relies on a complex inversion that aims at connecting a fully controlled input to the deterministic interference of the light field scrambled by the device.At the moment,iterative phase retrieval protocols provide the fastest reconstructing frameworks,converging in a few tens of iterations.Exploiting the knowledge of speckle correlations,we construct a new phase retrieval algorithm that reduces the computational cost to a single iteration.Besides being faster,our method is practical because it accepts fewer measurements than state-of-the-art protocols.Thanks to reducing computation time by one order of magnitude,our result can be a step forward toward real-time optical imaging that exploits disordered devices.

关键词： optical Matrix algorithm

Extracellular Matrix and its therapeutic potential for cancer treatment

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Signal Transduction and Targeted Therapy 2021年第5期6卷 1439-1462页

作者： Jiacheng Huang Lele Zhang Dalong Wan Lin Zhou Shusen Zheng Shengzhang Lin Yiting Qiao Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryFirst Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China School of Medicine Zhejiang UniversityHangzhou 310003China NHC Key Laboratory of Combined Multi-Organ Transplantation Hangzhou 310003China Key Laboratory of the Diagnosis and Treatment of Organ Transplantation Research Unit of Collaborative Diagnosis and Treatment For Hepatobiliary and Pancreatic CancerChinese Academy of Medical Sciences(2019RU019)Hangzhou 310003China Key Laboratory of Organ Transplantation Zhejiang ProvinceHangzhou 310003China Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College Hangzhou 310000China

The extracellular Matrix(ECM)Is one of the major components of tumors that plays multiple crucial roles,including mechanical support,modulation of the microenvironment,and a source of signaling molecules.The quantity and cross-linking status of ECM components are major factors determining tissue stiffness.During tumorigenesis,the interplay between cancer cells and the tumor microenvironment(TME)often results in the stiffness of the ECM,leading to aberrant mechanotransduction and further malignant transformation.Therefore,a comprehensive understanding of ECM dysregulation in the TME would contribute to the discovery of promising therapeutic targets for cancer treatment.Herein,we summarized the knowledge concerning the following:(1)major ECM constituents and their functions in both normal and malignant conditions;(2)the interplay between cancer cells and the ECM in the TME;(3)key receptors for mechanotransduction and their alteration during carcinogenesis;and(4)the current therapeutic strategies targeting aberrant ECM for cancer treatment.

关键词： therapeutic cancer Matrix

Overcoming power efficiency limitation of white fluorescence light-emitting diodes via multilevel-hydrogen-bond Matrix

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Aggregate 2023年第6期4卷 180-190页

作者： Ruiming Du Peng Ma Yi Man Yudong Pang Chunbo Duan Chunmiao Han Jing Zhang Chenhui Cao Ying Wei Xinfeng Shui Hui Xu Key Laboratory of Functional Inorganic Material Chemistry Ministry of Education and School of Chemistry and Material ScienceHeilongjiang UniversityHarbinP.R.China Anhui Sholon New Material Technology Co. Ltd.ChuzhouP.R.China

High power efficiency and low efficiency roll-off at practical luminance are two requirements for new-generation energy-saving lighting technologies,which are still bottlenecks of thermally activated delayed fluorescence(TADF)white organic light-emitting diodes(WOLED),despite the advantages of TADF materials and devices in low cost and high sustainability.Herein,we developed a spiro phosphine oxide host named SSOXSPO,which can form multiple and multidirectional intermolecular hydrogen bonds(IHB).The resulted multilevel IHB network integrates long-range ordered and short-range disordered alignments for suppressing triplet-polaron quenching(TPQ)and triplet-triplet annihilation(TTA).Electronic characteristics of SSOXSPO Matrix are further regulated,leading to the optimal exciton allocation through balancing energy and charge transfer.As consequence,using SSOXSPO as host,the single-emissive-layer TADF WOLEDs realized the record performance,including ultralow operation voltage as∼4.0 V,power efficiency beyond fluorescent tube(70.1 lm W−1)and negligible external quantum efficiency roll-off(3%)at 1000 nits for indoor lighting.This work demonstrates that multiple interplays supported by host Matrixes in TADF WOLEDs can facilitate the synergistic effects of TADF emitters on 100%exciton utilization.

关键词： hydrogen bond Matrix power efﬁciency thermally activated delayedﬂuorescence white organic light-emitting diode

Sequencing analysis of Matrix metalloproteinase 7-induced genetic changes in Schwann cells

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Neural Regeneration Research 2020年第11期15卷 2116-2122页

作者： Pan-Jian Lu Gang Wang Xiao-Dong Cai Ping Zhang Hong-Kui Wang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina

Previous research revealed the positive activity of Matrix metalloproteinase 7(MMP7) on migration and myelin regeneration of Schwa nn cells(SCs). However, understanding of the molecular changes and biological activities induced by increased amounts of MMP7 in SCs remains limited. To better understand the underlying molecular events, primary SCs were isolated from the sciatic nerve stump of newborn rats and cultured with 10 nM human MMP7 for 24 hours. The results of genetic testing were analyzed at a relatively relaxed threshold value(fold change ≥ 1.5 and P-value < 0.05). Upon MMP7 exposure, 149 genes were found to be upregulated in SCs, whereas 133 genes were downregulated. Gene Ontology analysis suggested that many differentially expressed molecules were related to cellular processes, single-organism processes, and metabolic processes. Kyoto Enrichment of Genes and Genomes pathway analysis further indicated the critical involvement of cell signaling and metabolism in MMP7-induced molecular regulation of SCs. Results of Ingenuity Pathway Analysis(IPA) also revealed that MMP7 regulates biological processes, molecular functions, cellular components, diseases and functions, biosynthesis, material metabolism, cell movement, and axon guidance. The outcomes of further analysis will deepen our comprehension of MMP7-induced biological changes in SCs. This study was approved by the Laboratory Animal Ethics Committee of Nantong University, China(approval No. 20190225-004) on February 27, 2019.

关键词： bioinformatic analysis ingenuity pathway analysis Matrix metalloproteinase 7 peripheral nerve regeneration RNA sequencing Schwann cells sciatic nerve injury

Extracellular Matrix and biomimetic engineering microenvironment for neuronal differentiation

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Neural Regeneration Research 2020年第4期15卷 573-585页

作者： Deepak Jain Sabrina Mattiassi Eyleen L.Goh Evelyn K.F.Yim Department of Chemical Engineering University of WaterlooWaterlooOntarioCanada Neuroscience Academic Clinical Programme Duke-NUS Medical SchoolSingapore

Extracellular Matrix(ECM)influences cell differentiation through its structural and biochemical properties.In nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fibrous,or tubular forms encompassing specific molecular compositions.In addition to contact guidance,ECM composition and structures also exert its effect on neuronal differentiation.This short report reviewed the native ECM structure and composition in central nervous system and peripheral nervous system,and their impact on neural regeneration and neuronal differentiation.Using topographies,stem cells have been differentiated to neurons.Further,focussing on engineered biomimicking topographies,we highlighted the role of anisotropic topographies in stem cell differentiation to neurons and its recent temporal application for efficient neuronal differentiation.

关键词： biomimetic platforms biophysical cues contact guidance extracellular Matrix neuronal development neural regeneration neural stem cell niche neuronal differentiation neuronal maturation stem cell topography