基于上消化道内镜活检的快速检测法诊断十二指肠乳糖酶缺乏

作     者：Kuokkanen M. Myllyniemi M. Vauhkonen M. P.Sipponen 宋平 

作者机构：Dept.of PathologyHelsinki University Central Hospital02740 EspooFrance Dr. 

出 版 物：《世界核心医学期刊文摘（胃肠病学分册）》 (Core Journals in Gastroenterology)

年 卷 期：2006年第2卷第12期

页      面：27-27页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：乳糖酶缺乏 上消化道 快速检测法 内镜活检 乳糜泻 成人型 阴性结果 试验结果 双糖酶 显色反应 

摘      要：Background and study aims:The usefulness of a new quick test for endoscopic diagnosis of adult-type hypolactasia was tested in duodenal *** this test,an endoscopic biopsy from the postbulbar duodenum is incubated with lactose on a test plate,and a color reaction develops within 20 min as a result of hydrolyzed lactose(a positive result) in patients with normolactasia,whereas no reaction(a negative result) develops in patients with severe *** and methods:Two postbulbar duodenal biopsies were taken from 80 prospectively enrolled adult outpatients with *** biopsies were used for the Quick Lactase Test(Biohit PLC,Helsinki,Finland) and in biochemical disaccharidase(lactase,sucrase,and maltase) *** addition,the C/T-13910 genotype was determined from DNA extracted from gastric antral biopsies using polymerase chain reaction sequencing in genomic analysis of adult-type ***:Twenty-one of 22 patients(95% ;95% CI,87-100%) with biochemical lactase activity 10 U/g protein,but none of the 58 patients with lactase activity of 10 U/g protein or more had a negative result in the Quick Lactase *** of the 80 patients(9% ;95% CI,3-15%) had a Quick Lactase Test result that indicated mild hypolactasia(a mild color reaction) .All patients with celiac disease(n = 6) had a negative Quick Lactase Test *** of 74 patients(six patients with celiac disease were excluded) had a CC-13910 genotype in genomic testing,indicating adult-type *** of them had negative test results with the Quick Lactase ***-six patients had a TT genotype,indicating normolactasia,and none of these patients had a negative test result in the Quick Lactase *** of 39 patients(15% ;95% CI,4-27%) with a CT genotype had a negative result in the Quick Lactase ***:The Quick Lactase Test effectively identifies patients with severe duodenal *** comparison with CC(adult-type hypolactasia) and TT individuals(n