Study on Formation and Inhibition Mechanism of Biofilm of Pig Actinobacillus pleuropneumoniae

作     者：Yanfei LIU Jiande YANG 

作者机构：College of Animal Science and Medicine Tianjin Agricultural University 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2019年第8卷第2期

页      面：119-121,148页

学科分类：09[农学] 

基　　金：Supported by Tianjin Natural Science Foundation Project(07JCYBJC16000) Key Technologies Integration and Students' Comprehensive Ability Improvement Project of Animal Hospital Affiliated to Tianjin Agricultural University(ZH004901) 

主　　题：Pig A. pleuropneumoniae Biofilm (BF) Gene regulation Formation mechanism Inhibition 

摘      要：Pig Actinobacillus pleuropneumoniae(App) could induce chronic respiratory tract infection in pigs, which causes major economic losses on pig industry. Bacterial biofilm(BBF) is bacterial community adsorbed on the surface of biomaterials or body cavity, to protect bacteria escape, and recurrent outbreaks of related infectious diseases and chronic infections resulting therefrom are called bacterial biofilm diseases. App BF belongs to polymers with spatial structure in vitro, and its formation is regulated by multiple genes. Among them, gene deletion of the key component TolC of multidrug efflux pumps and type I secretion systems causes that App BF adhesion weakens; gene deletion of catalytic core ClpP of Clp proteolytic complex induces the inhibition of BF formation; outer membrane lipoprotein VacJ of App promotes BF formation; gene deletion of active enzyme LuxS enhances the formation of App BF and decreases bacterial adhesion ability; gene deletion of Adh obviously declines bacterial accumulation, BF formation and adhesion to host cells. In this paper, BF formation or inhibition mechanism in App is elaborated from molecular level, which could provide reference basis for exploring the prevention of its biofilm diseases.