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ArcA Gene Expression Level of Actinobacillus pleuropneumoniae in Anaerobic Culture

畜牧兽医学报 2009年第S1期40卷 34-38页

作者： DUAN Li-li WEN Xin-tian CAO San-jie HUANG Xiao-bo Laboratory of Animal Infectious Disease and Microarray Key Laboratory of Animal Disease and Human Health of Sichuan ProvinceCollege of Animal MedicineSichuan Agricultural University

In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic *** levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal *** expression of ArcA was undetectable until about 3 hours under standard culture conditions,but it was promptly and highly expressed in anaerobic *** results are consistent with ArcA being a potential virulence gene of Actinobacillus pleuropneumoniae,and likely playing an important role in pathogenesis caused by this organism.

关键词： semi-quantitative RT-PCR Actinobacillus pleuropneumoniae ArcA gene anaerobic stress

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Presence of <i>Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus parasuis and Mycoplasma hyopneumoniae</i>in upper respiratory tract of swine in farms from Aguascalientes, Mexico

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Open Journal of Animal Sciences 2013年第2期3卷 132-137页

作者： Abraham Loera-Muro Francisco J. Avelar-González Víctor M. Loera-Muro Mario Jacques Alma L. Guerrero-Barrera Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México Groupe de recherche sur les maladies infectieuses du porc Faculté de Médecine Vétérinaire Université de Montréal St-Hyacinthe Canada Laboratorio de Biología Celular y Tisular Departamento de Morfología Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México

Respiratory diseases are one of the most important health problems in pig herds. The porcine respiratory disease complex (PRDC) is the term used to describe pneumonic diseases caused by multiple infectious agents that provoke weight loss in animals or death. In the PRDC multiple pathogens (bacteria and/or viruses) work in combination to induce this respiratory disease. Within this complex, Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus parasuis and Mycoplasma hyopneumoniae are the main bacterial pathogens involved in great economic losses to the swine industry. The aim of this work was to estimate the presence of A. pleuropneumoniae, S. suis, P. multocida, B. bronchiseptica, H. parasuis and M. hyopneumoniae in the upper respiratory tract of pigs in representative swine farms inAguascalientes,Mexico, using PCR technique. The study was performed in 14 swine farms. We obtained a total of 212 nasal swabs. Near 20% of samples were positive for A. pleuropneumoniae (located in the 79% of farms);17% were positive for S. suis (in 86% of farms), of these, 3% were S. suis serovar 2;30% were positive for H. parasuis (93% of farms);23% of the samples to P. multocida (in 79% of farms);and 19% to M. hyopneumoniae (in 64% of farms). B. bronchiseptica was not detected in this study. The results obtained show that bacterial pathogens of PRDC were present in the upper respiratory tract of pigs in all farms studied;therefore, these pathogens are widely disseminated in pig farms of Aguascalientes, Mexico.

关键词： Porcine Respiratory Disease Complex Actinobacillus pleuropneumoniae Streptococcus suis Pasteurella multocida Bordetella bronchiseptica Haemophilus parasuis Mycoplasma hyopneumoniae

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Study on Formation and Inhibition Mechanism of Biofilm of Pig Actinobacillus pleuropneumoniae

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Agricultural Biotechnology 2019年第2期8卷 119-121,148页

作者： Yanfei LIU Jiande YANG College of Animal Science and Medicine Tianjin Agricultural University

Pig Actinobacillus pleuropneumoniae(App) could induce chronic respiratory tract infection in pigs, which causes major economic losses on pig industry. Bacterial biofilm(BBF) is bacterial community adsorbed on the surface of biomaterials or body cavity, to protect bacteria escape, and recurrent outbreaks of related infectious diseases and chronic infections resulting therefrom are called bacterial biofilm diseases. App BF belongs to polymers with spatial structure in vitro, and its formation is regulated by multiple genes. Among them, gene deletion of the key component TolC of multidrug efflux pumps and type I secretion systems causes that App BF adhesion weakens; gene deletion of catalytic core ClpP of Clp proteolytic complex induces the inhibition of BF formation; outer membrane lipoprotein VacJ of App promotes BF formation; gene deletion of active enzyme LuxS enhances the formation of App BF and decreases bacterial adhesion ability; gene deletion of Adh obviously declines bacterial accumulation, BF formation and adhesion to host cells. In this paper, BF formation or inhibition mechanism in App is elaborated from molecular level, which could provide reference basis for exploring the prevention of its biofilm diseases.

关键词： Pig A. pleuropneumoniae Biofilm (BF) Gene regulation Formation mechanism Inhibition

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Genetic Evolution Analysis of Actinobacillus pleuropneumoniae

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Agricultural Biotechnology 2019年第2期8卷 113-115,118页

作者： Yu LIANG Yanfei LIU Jiande YANG College of Animal Science and Veterinary Medicine Tianjin Agricultural University

In order to explore the genetic evolution of Actinobacillus pleuropneumoniae(App) in different countries and clarify the relationships among different App in each region, the 16 S rRNA gene of App in the NCBI nucleotide database was analyzed and compared by the bioinformatics method. The phylogenetic tree was constructed after tailoring alignment. The results showed that a stable genetic phenomenon was indicated in the evolutionary process of App. The isolates derived from China were clustered and showed a high degree of conservation. They had a certain genetic relationship with the British and American strains, but had far relationship with the strains from Japan which was a neighboring country of China. The isolates from different countries in the Eurasian continent shared high homology. The isolates of the two regions originated from common ancestors.

关键词： Actinobacillus pleuropneumoniae Genetic evolution Phylogenetic tree Homology comparison Origin Relationship

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