Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

作     者：Birendra Kumar Umesh Kumar Hemant Kumar Yadav 

作者机构：CSIR-Central Institute of Medicinal and Aromatic Plant Sciences P.O.CIMAP Near Kukrail Picnic Spot CSIR-National Botanical Research Institute Rana Pratap Marg Academy of Scientific and Innovative Research (AcSIR) 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2015年第3卷第4期

页      面：335-342页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

主　　题：EST–SSRs Genetic diversity Mentha piperita Polymorphic information content Transferability 

摘      要：EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.