检索结果-南通市图书馆

Identification of est–ssrs and molecular diversity analysis in Mentha piperita

The Crop Journal 2015年第4期3卷 335-342页

作者： Birendra Kumar Umesh Kumar Hemant Kumar Yadav CSIR-Central Institute of Medicinal and Aromatic Plant Sciences P.O.CIMAP Near Kukrail Picnic Spot CSIR-National Botanical Research Institute Rana Pratap Marg Academy of Scientific and Innovative Research (AcSIR)

est sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ests were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 ssrs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat ssrs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified ssrs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed ssrs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

关键词： est–ssrs Genetic diversity Mentha piperita Polymorphic information content Transferability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：