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Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by <i>Lentinus squarrosulus</i>

Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by <i>Lentinus squarrosulus</i&gt

作     者:Rahayu Ahmad Najeeb Kaid Nasser Al-Shorgani Aidil Abdul Hamid Wan Mohtar Wan Yusoff Fauzi Daud 

作者机构:School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia Department of Applied Microbiology Faculty of Applied Sciences Taiz University Taiz Yemen 

出 版 物:《Advances in Bioscience and Biotechnology》 (生命科学与技术进展(英文))

年 卷 期:2013年第4卷第12期

页      面:1079-1085页

学科分类:081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

主  题:Lentinus squarrosulus Response Surface Methodology (RSM) Exopolysaccharide Submerged Culture Central Composite Design (CCD) 

摘      要:The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81;sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

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