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Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by Lentinus squarrosulus

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Advances in Bioscience and Biotechnology 2013年第12期4卷 1079-1085页

作者： Rahayu Ahmad Najeeb Kaid Nasser Al-Shorgani Aidil Abdul Hamid Wan Mohtar Wan Yusoff Fauzi Daud School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia Department of Applied Microbiology Faculty of Applied Sciences Taiz University Taiz Yemen

The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81;sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

关键词： Lentinus squarrosulus Response Surface Methodology (RSM) Exopolysaccharide Submerged Culture Central Composite Design (CCD)

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Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by Lentinus squarrosulus

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Advances in Bioscience and Biotechnology 2015年第8期6卷 526-536页

作者： Felicia N. Anike Omoanghe S. Isikhuemhen Dietrich Blum Hitoshi Neda Mushroom Biology and Fungal Biotechnology Laboratory North Carolina A&T State University Greensboro USA Forestry and Forest Products Research Institute (FFPRI) Tsukuba Japan

Lentinus squarrosulus Mont. is an emerging tropical white rot basidiomycete, with nutritional and medicinal benefits. Low levels of commercial cultivation of the mushrooms limit their availability for use as food and medicine. Mycelia from submerged fermentation are a suitable alternative to the mushroom from L. squarrosulus. Three strains, 340, 339 and 218, were studied to determine optimum growth conditions for mycelia mass and crude exo-polysaccharides (CEPS) production. The experiments were conducted in a completely randomized design (CRD) with a factorial structure. Nutrients involving 8 carbon and 8 nitrogen sources were screened, and concentrations of the best sources were optimized. Optimized nutrients, interaction between strains and other parameters such as agitation and medium volume were investigated to obtain optimum fermentation conditions for biomass and CEPS production. Biomass yield varied among strains depending on carbon or nitrogen nutrient sources. Starch and yeast extract at 30 and 25 g/L were identified as the most important nutrients in mycelia and CEPS production. Nutrient optimization resulted in a 3-fold increase in mycelia mass: 12.8, 10.0 and 15.3 g/L in strains 340, 339 and 218 respectively. There was a significant interaction between strain, agitation, and volume (p p = 0.02). Static conditions favored more polysaccharide production. Optimized fermentation conditions resulted in very high increase in biomass: 238.1, 266.9 and 185.0 g/L in strains 340, 339 and 218 respectively. Results obtained could be useful in modeling fermentation systems for large-scale production of mycelia mass, CEPS and other bio-products from L. squarrosulus.

关键词： Mycelia Mass Carbon and Nitrogen Requirements Exopolysaccharides Lentinus squarrosulus Submerged Fermentation

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Antioxidant Properties and Glucan Compositions of Various Crude Extract from Lentinus squarrosulusMycelial Culture

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Advances in Bioscience and Biotechnology 2014年第10期5卷 805-814页

作者： Rahayu Ahmad Sutha Muniandy Nur Iffah Abdullah Shukri Siti Mariatul Usyida Alias Aidil Abdul Hamid Wan Mohtar Wan Yusoff Sahidan Senafi Fauzi Daud School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Malaysia

Various crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan and carbohydrate compound analysis. Crude extract from ethanol, cold and hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, total flavonoid compound, total carbohydrate content and total glucan compositions. The antioxidant activity of the crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth was determined by 2,2’ diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene linoleic acid assay. Crude extracted from culture broth EPS EE recorded the highest radical scavenging effects at 20 mg/mL (85%) which is closer to the control antioxidants (ascorbic acid 97%;BHT 92.4%;BHA 93.6%). Both the ethanol extract (IPS EE and EPS EE) exhibited the highest β-carotene linoleic acid oxidation by recording IC50 values of 0.6 and 0.65 mg/mL respectively. IPS EE (0.59 mg/g) and EPS WE (0.58 mg/g) showed high flavonoid content whereas total phenol content was high in EPS WE (0.85 mg/g) and IPS EE (0.84 mg/g). Total carbohydrate concentrations were high in IPS HWE and IPS CWE with 19.1 and 16.3 mg/mL respectively and these data correlate with the β-glucan content in both extracts. The highest β-glucan content found in IPS CWE and IPS HWE were 12.2 and 11.4% w/w respectively.

关键词： Lentinus squarrosulus β-Glucan Antioxidant Polysaccharides Submerged Fermentation

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一株野生翘鳞香菇的培养特性研究

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湖北农业科学 2015年第8期54卷 1923-1927页

作者：张国广张泽宏赵晓丹洪雅珍邹金美闽南师范大学生物科学与技术学院福建漳州363000

以野外采集分离的一株野生翘鳞香菇（Lentinus squarrosulus Mont．）为研究对象，探讨了培养基碳源、氮源、碳氮比、pH和温度对翘鳞香菇菌丝生长的影响，同时进行了人工驯化栽培。结果表明，翘鳞香菇的菌丝在生长过程中培养特性明显．... 详细信息

以野外采集分离的一株野生翘鳞香菇（Lentinus squarrosulus Mont．）为研究对象，探讨了培养基碳源、氮源、碳氮比、pH和温度对翘鳞香菇菌丝生长的影响，同时进行了人工驯化栽培。结果表明，翘鳞香菇的菌丝在生长过程中培养特性明显．培养基最适碳源是葡萄糖或麦芽糖，最适氮源是酵母浸膏，最适碳氮比是10：1—20：1，最适pH为5．5～6．0，最适生长温度是30℃左右。在此培养条件下，试验成功获得了翘鳞香菇的大型子实体。

关键词：关键词:翘鳞香菇(Lentinus squarrosulus Mont.) 培养特性人工栽培

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