Effect of diets supplemented with linseed alone or combined with vitamin E and selenium or with plant extracts,on Longissimus thoracis transcriptome in growing-finishing Italian Large White pigs

作     者：Rubina Sirri Marika Vitali Paolo Zambonelli Giulia Giannini Martina Zappaterra Domenico Pietro Lo Fiego Dalal Sami Roberta Davoli 

作者机构：Interdepartmental Centre for Industrial Agrifood Research (CIRI- AGRO)University of Bologna Department of Agricultural and Food Sciences (DISTAL)University of Bologna Department of Life SciencesUniversity of Modena and Reggio Emilia Interdepartmental Research Centre for Agri-Food Biological Resources Improvement and Valorisation (BIOGEST-SITEIA)University of Modena and Reggio Emilia 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2019年第10卷第1期

页      面：159-171页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：funded by Regione Emilia-Romagna POR-FESR 2014-2020"Innovare la filiera suina mediante la valorizzazione di sottoprodotti vegetali e l’impiego di avanzate tecnologie "omiche" e di processo per la produzione sostenibile di carne e salumi ad impatto positivo sulla salute"-Green Charcuterie grant n.PG/2015/730542 

主　　题：Diet supplementation Functional analysis Gene expression Linseed Muscle transcriptome n-3 PUFA Pigs Plant extracts Polyphenols Vitamin E 

摘      要：Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat *** composition has been also demonstrated to influence the gene expression with effect on biological processes and ***,the knowledge on the effect of nutrients at the molecular level is *** particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so ***-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene *** present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard ***: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard *** diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative *** few genes involved in extracellular matrix(ECM)organization were upregulated by the ***,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub *** particular,IGF2 wa