Eicosane, pentadecane and palmitic acid: The effects in in vitro wound healing studies

作     者：Xin Qi Chuah Patrick Nwabueze Okechukwu Farahnaz Amini Swee Sen Teo 

作者机构：Department of BiotechnologyFaculty of Applied SciencesUCSI University School of Healthy AgingMedical Aesthetics and Regenerative MedicineUCSI University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2018年第8卷第10期

页      面：490-499页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Centre of Excellence for Research Value Innovation and Entrepreneurship Research Grant Scheme UCSI University(UCSI-CERVIE-RGS Proj-in-FAS 039) 

主　　题：Eicosane Pentadecane Palmitic acid Wound healing TNF-α IL-12 

摘      要：Objective: To examine the wound healing properties of eicosane, pentadecane and palmitic acid by evaluating in term of anti-microbial, anti-inflammatory, proliferation, migration and collagen synthesis. Methods: Anti-microbial activities of Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa were evaluated by carrying out disk diffusion and agar well diffusion methods. Growth rate of tested bacteria was also evaluated for 8 h in conjunction with the sample drugs. Besides, U937 cell lines were used as model study for realtime mRNA genes expression studies of TNF-毩 and IL-12 under the treatment. Proliferation, migration and collagen content synthesis were carried out on human dermal fibroblast. Results: None of the sample drugs possessed significant inhibition of bacteria tested in this study both in disk diffusion and agar well diffusion methods. In contrary, significantly low expressed mRNA gene expression levels of TNF-毩 and IL-12 were found under the treatment of respective drugs. Meanwhile in proliferation, migration and hydroxyproline content analysis, all the sample drugs showed no significant positive stimulation. Conclusions: This study therefore explains that apart from their potential in downregulating pro-inflammatory cytokines, these three compounds which were examined individually may not be good candidates in promoting wound healing.