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New procedure for the differential display of mitochondria RNA

New procedure for the differential display of mitochondria RNA

作     者:LING Xingyuan, ZHOU Peijiang and ZHU Yingguo1. Institute of Genetics , College of Life Science , Wuhan University , Wuhan 430072, China 2. The Center of Biotechnology , Anhui Agriculture University , Hefei 230036, China Corresponding author 

作者机构:Institute of Genetics College of Life Science Wuhan University Wuhan China The Center of Biotechnology Anhui Agriculture University Hefei China 

出 版 物:《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期:1999年第44卷第16期

页      面:1502-1505页

核心收录:

学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

主  题:rice mitochondria RNA. mRNA differential display. amplification fragment length polymorphism (AFLP ) . 

摘      要:A novel approach for the differential display of mitochondria RNA has been described . The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the tem-

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