New procedure for the differential display of mitochondria RNA
New procedure for the differential display of mitochondria RNA作者机构:Institute of Genetics College of Life Science Wuhan University Wuhan China The Center of Biotechnology Anhui Agriculture University Hefei China
出 版 物:《Chinese Science Bulletin》 (科学通报(英文版))
年 卷 期:1999年第44卷第16期
页 面:1502-1505页
核心收录:
学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
主 题:rice mitochondria RNA. mRNA differential display. amplification fragment length polymorphism (AFLP ) .
摘 要:A novel approach for the differential display of mitochondria RNA has been described . The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the tem-