Cross-linked self-assembling peptide scaffolds
Cross-linked self-assembling peptide scaffolds作者机构：IRCCS Casa Sollievo della Sofferenza Opera di San Pio da Pietralcina Viale Capuccini I San Giovanni Rotondo Foggia 71013 Italy Center for Nanomedicine and Tissue Engineering (CNTE) A. O. Ospedale Niguarda Cd Granda Piazza dell' Ospedale Maggiore 3 Milan 20162 Italy The Molecular Foundry Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley California 94720 USA
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2018年第11卷第1期
页 面：586-602页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 081402[工学-结构工程] 0814[工学-土木工程]
基 金：funded by Fondazione Cariplo La Colonna Onlus the "Ricerca Corrente 2015-2016" funding granted by the Italian Ministry of Health and by the "5 x 1000" voluntary contributions supported by a fellowship granted by Vertical Onlus. Raman, XRD, FTIR and WAXS Advanced Light Source and at the Molecular Foundry, at the Lawrence Berkeley National Laboratory supported by the Office of Science
主 题：self-assembling peptide co-assembling peptide cross-linking sulfo-SMCC supramolecularself-assembly
摘 要：Self-assembling peptides （SAPs） are synthetic bioinspired biomaterials that can be feasibly multi-functionalized for cell transplantation and/or drug delivery therapies. Despite their superior biocompatibility and ease of scaling-up for production, they are unfortunately hampered by weak mechanical properties due to transient non-covalent interactions among and within the self-assembled peptide chains, thus limiting their potential applications as fillers, hemostat solutions, and fragile scaffolds for soft tissues. Here, we have developed and characterized a cross-linking strategy that increases both the stiffness and the tailorability of SAP hydrogels, enabling the preparation of transparent flexible threads, discs, channels, and hemispherical constructs. Empirical and computational results, in close agreement with each other, confirmed that the cross-linking reaction does not affect the previously self-assembled secondary structures. In vitro tests also provided a first hint of satisfactory biocompatibility by favoring viability and differentiation of human neural stem cells. This work could bring self-assembling peptide technology to many applications that have been precluded so far, especially in regenerative medicine.
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