Toxic effect of acrylamide on the development of hippocampal neurons of weaning rats

作     者：Sheng-min Lai Zi-ting Gu Meng-meng Zhao Xi-xia Li Yu-xin Ma Li Luo Jing Liu 

作者机构：Department of Human Anatomy and HistoembryologySchool of Basic CoursesGuangdong Pharmaceutical UniversityGuangzhouGuangdong ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2017年第12卷第10期

页      面：1648-1654页

核心收录：

学科分类：100405[医学-卫生毒理学] 0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the Guangdong Provincial Department of Science and Technology in China No.2016A020225007 

主　　题：nerve regeneration acrylamide hippocampus neurons developmental toxicity growth associated protein 43 synaptophysin weaning rats dentate gyrus protein developmental neurobiology neural regeneration 

摘      要：Although numerous studies have examined the neurotoxicity of acrylamide in adult animals,the effects on neuronal development in the embryonic and lactational periods are largely unknown.Thus,we examined the toxicity of acrylamide on neuronal development in the hippocampus of fetal rats during pregnancy.Sprague-Dawley rats were mated with male rats at a 1：1 ratio.Rats were administered 0,5,10 or 20 mg/kg acrylamide intragastrically from embryonic days 6–21.The gait scores were examined in pregnant rats in each group to analyze maternal toxicity.Eight weaning rats from each group were also euthanized on postnatal day 21 for follow-up studies.Nissl staining was used to observe histological change in the hippocampus.Immunohistochemistry was conducted to observe the condition of neurites,including dendrites and axons.Western blot assay was used to measure the expression levels of the specific nerve axon membrane protein,growth associated protein 43,and the presynaptic vesicle membrane specific protein,synaptophysin.The gait scores of gravid rats significantly increased,suggesting that acrylamide induced maternal motor dysfunction.The number of neurons,as well as expression of growth associated protein 43 and synaptophysin,was reduced with increasing acrylamide dose in postnatal day 21 weaning rats.These data suggest that acrylamide exerts dose-dependent toxic effects on the growth and development of hippocampal neurons of weaning rats.